Evaluation of the Sterility of Press'n Seal Cling Film for Use in Rodent Surgery

Emmer, Kathryn M; Celeste, Natalie A; Bidot, Willie A; Perret-Gentil, Marcel I; Malbrue, Raphael A.

doi:10.30802/aalas-jaalas-18-000096

Cited by 12 publications

(2 citation statements)

References 17 publications

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“…Using a translucent surgical drape such as Press’N Seal Cling Film (Glad Press’n Seal, Glad Products, Oakland, CA) or Tegaderm (3M Corporation, St. Paul, MN), as opposed to fabric or medical surgical drapes, permits continuous skin coloration monitoring during surgical procedures when it is also critical to maintain a sterile surgical field ( Figure 6 ). 147 Although useful, monitoring skin coloration changes is not a sensitive lung gas exchange efficacy indicator; pulse oximetry is a more sensitive indicator.…”

Section: Anesthetic Monitoringmentioning

confidence: 99%

Mouse Anesthesia: The Art and Science

et al. 2021

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There is an art and science to performing mouse anesthesia, which is a significant component to animal research. Frequently, anesthesia is one vital step of many over the course of a research project spanning weeks, months, or beyond. It is critical to perform anesthesia according to the approved research protocol using appropriately handled and administered pharmaceutical-grade compounds whenever possible. Sufficient documentation of the anesthetic event and procedure should also be performed to meet the legal, ethical, and research reproducibility obligations. However, this regulatory and documentation process may lead to the use of a few possibly oversimplified anesthetic protocols used for mouse procedures and anesthesia. Although a frequently used anesthetic protocol may work perfectly for each mouse anesthetized, sometimes unexpected complications will arise, and quick adjustments to the anesthetic depth and support provided will be required. As an old saying goes, anesthesia is 99% boredom and 1% sheer terror. The purpose of this review article is to discuss the science of mouse anesthesia together with the art of applying these anesthetic techniques to provide readers with the knowledge needed for successful anesthetic procedures. The authors include experiences in mouse inhalant and injectable anesthesia, peri-anesthetic monitoring, specific procedures, and treating common complications. This article utilizes key points for easy access of important messages and authors’ recommendation based on the authors’ clinical experiences.

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Section: Anesthetic Monitoringmentioning

confidence: 99%

Mouse Anesthesia: The Art and Science

et al. 2021

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“…For all surgeries, mice were anesthetized with 1.5-2.5% isoflurane and 1L/min oxygen. The animal was then placed in a stereotaxic apparatus with the animal resting on a water heater pad and draped with Press'n Seal Cling Film 28 to maintain body temperature and provide a sterile field. Ophthalmic ointment was applied to the eyes.…”

Section: Aav Injection Lens Implant and Microscope Baseplate Surgeriesmentioning

confidence: 99%

MiniFAST: A sensitive and fast miniaturized microscope forin vivoneural recording

Juneau

Duret

Chu

et al. 2020

Preprint

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Observing the activity of large populations of neurons in vivo is critical for understanding brain function and dysfunction. The use of fluorescent genetically-encoded calcium indicators (GECIs) in conjunction with miniaturized microscopes is an exciting emerging toolset for recording neural activity in unrestrained animals. Despite their potential, current miniaturized microscope designs are limited by using image sensors with low frame rates, sensitivity, and resolution. Beyond GECIs, there are many neuroscience applications which would benefit from the use of other emerging neural indicators, such as fluorescent genetically-encoded voltage indicators (GEVIs) that have faster temporal resolution to match neuron spiking, yet, require imaging at high speeds to properly sample the activity-dependent signals. We integrated an advanced CMOS image sensor into a popular open-source miniaturized microscope platform. MiniFAST is a fast and sensitive miniaturized microscope capable of 1080p video, 1.5 μm resolution, frame rates up to 500 Hz and high gain ability (up to 70 dB) to image in extremely low light conditions. We report results of high speed 500 Hz in vitro imaging of a GEVI and ~300 Hz in vivo imaging of transgenic Thy1-GCaMP6f mice. Finally, we show the potential for a reduction in photobleaching by using high gain imaging with ultra-low excitation light power (0.05 mW) at 60 Hz frame rates while still resolving Ca2+ spiking activity. Our results extend miniaturized microscope capabilities in high-speed imaging, high sensitivity and increased resolution opening the door for the open-source community to use fast and dim neural indicators.

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Targeted AAV Transduction for Fluorescent Imaging of the Tripartite Synapse

Christie,

Reynolds

2024

Neuromethods

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Evaluation of the Sterility of Press'n Seal Cling Film for Use in Rodent Surgery

Cited by 12 publications

References 17 publications

Mouse Anesthesia: The Art and Science

Mouse Anesthesia: The Art and Science

MiniFAST: A sensitive and fast miniaturized microscope forin vivoneural recording

Targeted AAV Transduction for Fluorescent Imaging of the Tripartite Synapse

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