Evaluation of the suitability of vacutainers for storage of nutrient and dissolved organic carbon analytes in water samples

Jiang, Shan; Kavanagh, Mark; Rocha, Carlos

doi:10.3318/bioe.2017.01

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…In each reactor, four samplings were conducted at 1 h intervals. The collected samples were filtered with Millipore syringe filters (0.22 µm pore size) and stored in acid prewashed plastic bottles for dissolved inorganic nitrogen (DIN) species and precombusted glass vials for DOC and chromophoric dissolved organic matter (CDOM) determinations and kept at −20 • C prior to laboratory analyses to avoid potential microbial degradation of the samples (Jiang et al, 2017). Besides the collection of water samples, DO concentrations at both the input solution and the outflow stream were directly measured with a portable probe (WTW Multi 3630).…”

Section: Laboratory Ftr Simulationmentioning

confidence: 99%

Response of Nitrate Processing to Bio-labile Dissolved Organic Matter Supply Under Variable Oxygen Conditions in a Sandy Beach Seepage Face

Jiang

Jin

et al. 2021

Front. Mar. Sci.

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Supply of bio-labile dissolved organic matter (DOM) has been assumed to be a key factor for the intensity of nitrate (NO3–) removal in permeable coastal sediments. In the present study, a series of flow through reactor experiments were conducted using glucose as a N-free bio-labile DOM source to permeable sediments from a sandy beach seepage face to identify its effect on benthic NO3– removal. The results revealed a shift from the dominance of NO3– production to removal processes when NO3– input concentration increased from 10 to 80 μM under oxic conditions. Sediment microbiota information suggests that nitrification (e.g., Nitrosomonas and Nitrososphaera) and denitrification (e.g., Marinobacter and Bacillus) were dominant pathways for benthic NO3– production and removal in the studied sediment. Compared with the active response of sediment microbiota to NO3– additions, the supply of glucose (approximately 300 μM final concentration added) did not significantly change the NO3– removal efficiency under aerobic conditions (dissolved oxygen saturation approximately 100%). Similarly, an insignificant increase of NO3– removal rate after glucose amendment of the circulating water was obtained when dissolved oxygen (DO) saturation decreased to approximately 70% in the input solution. When DO at the input solution was decreased to 30% saturation (sub-oxic conditions), the removal rate of NO3– in the group amended with glucose increased, suggesting that glucose stimulated denitrifiers. These results revealed that NO3– removal relied mainly on the anaerobic environment at particle surfaces, with a dependence on the sedimentary organic matter as an electron supplier under bulk aerobic conditions, while the bio-labile DOM was consumed mainly by aerobic respiration instead of stimulating NO3– reduction. However, the respiration triggered by the over-supply of bio-labile DOM reduced the DO in the porewater, likely depressing the activity of aerobic reactions in the permeable sediment. At this point, the benthic microbiota, especially potential denitrifiers, shifted to anaerobic reactions as the key to support nitrogen metabolism. The glucose amendment benefited NO3– reduction at this point, under sub-oxic conditions.

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Section: Laboratory Ftr Simulationmentioning

confidence: 99%

Response of Nitrate Processing to Bio-labile Dissolved Organic Matter Supply Under Variable Oxygen Conditions in a Sandy Beach Seepage Face

Jiang

Jin

et al. 2021

Front. Mar. Sci.

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“…The filtrate was stored in acidprewashed high-density polyethylene (HDPE) bottles at −20 • C environment for major ions, nutrients, and N stable isotope fractions. Water stable isotope samples were stored in air-tight vacutainers at 4 • C environment (Jiang et al, 2017). Occasionally, the volume of collected rainwater was limited.…”

Section: Sample Collectionmentioning

confidence: 99%

Nitrogen in Atmospheric Wet Depositions Over the East Indian Ocean and West Pacific Ocean: Spatial Variability, Source Identification, and Potential Influences

et al. 2021

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Atmospheric deposition is the dominant pathway for the loading of exogenous nitrogen (N) to open ocean. Here, rainwater samples were collected from 31 stations in the equatorial East Indian Ocean (EIO) and West Pacific Ocean (WPO) to explore the spatial variability of N species, potential sources, and related ecological influences. Among two oceans, nitrate (NO3–) and ammonium (NH4+) were the main components in the rainwater N inventory. NO3– concentrations varied from 0.19 to 100.5 μM, whereas NH4+ concentrations ranged from 0.54 to 110.6 μM. Among all stations, low concentrations of NO3– and NH4+ appeared in the remote ocean, whereas high concentrations were observed at the stations near the Malacca Strait and New Guinea, coupled with an enhancement of non-sea salt major ions, e.g., calcium ions (Ca2+) and sulfate (SO42–), revealing the influence from coastal human activities, such as coal and gasoline combustion. In the remote ocean, δ15N–NH4+ ranged from −5.7 to −9.3‰, whereas it dropped to -15.5‰ near coasts. A logarithmic decay between δ15N–NH4+ and NH4+ concentrations in rainwater samples was obtained, suggesting a shift from natural source (seawater emission) in oceanic precipitation events to anthropogenic source (chemical fertilizer volatilization and vehicle exhaust) in coastal rainwaters. δ15N–NO3– in the remote ocean varied between −1.7 and 0.4‰ with low levels found in the WPO, likely related to the ascending air flow driven by the Walker Circulation. In coastal oceans, δ15N–NO3– ranged from 1.5 to 3.5‰. The linkage between δ15N–NO3– and NO3– concentrations varied in two oceans, resulting from difference in biological and fossil fuel combustion contributions. Compared with ocean surface water, N in the rainwater was markedly enriched, suggesting that N from atmospheric wet depositions could rapidly enhance the dissolved N availability in ocean surface water. However, the N redundancy according to the Redfield–Brzezinski ratio (N:Si:P = 16:16:1) in the rainwater might benefit from the growth of N-preference phytoplankton species and microbes. As the first study on N concentrations, sources, and stoichiometry balance in rainwater over the equatorial WPO and EIO, the results could be a support to the global N budget estimation and oceanic primary production modeling.

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Denitrification-nitrification process in permeable coastal sediments: An investigation on the effect of salinity and nitrate availability using flow-through reactors

et al. 2021

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Evaluation of the suitability of vacutainers for storage of nutrient and dissolved organic carbon analytes in water samples

Cited by 5 publications

References 41 publications

Response of Nitrate Processing to Bio-labile Dissolved Organic Matter Supply Under Variable Oxygen Conditions in a Sandy Beach Seepage Face

Response of Nitrate Processing to Bio-labile Dissolved Organic Matter Supply Under Variable Oxygen Conditions in a Sandy Beach Seepage Face

Nitrogen in Atmospheric Wet Depositions Over the East Indian Ocean and West Pacific Ocean: Spatial Variability, Source Identification, and Potential Influences

Denitrification-nitrification process in permeable coastal sediments: An investigation on the effect of salinity and nitrate availability using flow-through reactors

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