EVALUATION OF THE SYMBIOTIC EFFECTIVENESS OF PEA (Pisum sativum L.) GENOTYPES IN POT EXPERIMENT

Zhukov, Vladimir A.; Асановна, Ахтемова Гульнар; Zhernakov, A. I.; Sulima, Anton S.; Shtark, O. Y.; Тихонович, И. А.

doi:10.15389/agrobiology.2017.3.607eng

Cited by 10 publications

(9 citation statements)

References 12 publications

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“…Under the conditions of this experiment, no positive effect of mycorrhiza on the growth parameters of pea during the vegetative and reproductive stages (I-V) was revealed. The data obtained here are consistent with numerous observations made by other authors who noted a low growth response of P. sativum to AMF mono-inoculation (Rivera-Becerril et al, 2002; Xavier & Germida, 2003; Borisov et al, 2004b; Desalegn et al, 2016; Zhukov et al, 2017). It was also previously shown that the effect of mycorrhiza was often not manifested under artificial lighting conditions (Konvalinková & Jansa, 2016), so it is likely that the illumination in the growth chambers used in this study was not sufficient for the growth response to occur.…”

Section: Discussionsupporting

confidence: 93%

“…However, P. sativum had a relatively low growth response to mono-inoculation with AMF compared to Medicago varia , Secale cereale or Hordeum vulgare , as was demonstrated in the geographical network experiments of the All-Russia Research Institute for Agricultural Microbiology (St. Petersburg) (Yurkov et al, 2017). Furthermore, many authors have described the complete absence of an increase of pea growth parameters under conditions of mono-inoculation with AMF (Rivera-Becerril et al, 2002; Xavier & Germida, 2003; Borisov et al, 2004b; Desalegn et al, 2016; Zhukov et al, 2017). Even pea genotypes having the highest growth response to double inoculation may not show a positive response to mono-inoculation with AMF (Borisov et al, 2004b).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Shtark

Puzanskiy

Avdeeva

et al. 2019

PeerJ

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Arbuscular mycorrhiza (AM) is known to be a mutually beneficial plant-fungal symbiosis; however, the effect of mycorrhization is heavily dependent on multiple biotic and abiotic factors. Therefore, for the proper employment of such plant-fungal symbiotic systems in agriculture, a detailed understanding of the molecular basis of the plant developmental response to mycorrhization is needed. The aim of this work was to uncover the physiological and metabolic alterations in pea (Pisum sativum L.) leaves associated with mycorrhization at key plant developmental stages. Plants of pea cv. Finale were grown in constant environmental conditions under phosphate deficiency. The plants were analyzed at six distinct time points, which corresponded to certain developmental stages of the pea: I: 7 days post inoculation (DPI) when the second leaf is fully unfolded with one pair of leaflets and a simple tendril; II: 21 DPI at first leaf with two pairs of leaflets and a complex tendril; III: 32 DPI when the floral bud is enclosed; IV: 42 DPI at the first open flower; V: 56 DPI when the pod is filled with green seeds; and VI: 90–110 DPI at the dry harvest stage. Inoculation with Rhizophagus irregularis had no effect on the fresh or dry shoot weight, the leaf photochemical activity, accumulation of chlorophyll a, b or carotenoids. However, at stage III (corresponding to the most active phase of mycorrhiza development), the number of internodes between cotyledons and the youngest completely developed leaf was lower in the inoculated plants than in those without inoculation. Moreover, inoculation extended the vegetation period of the host plants, and resulted in increase of the average dry weight per seed at stage VI. The leaf metabolome, as analyzed with GC-MS, included about three hundred distinct metabolites and showed a strong correlation with plant age, and, to a lesser extent, was influenced by mycorrhization. Metabolic shifts influenced the levels of sugars, amino acids and other intermediates of nitrogen and phosphorus metabolism. The use of unsupervised dimension reduction methods showed that (i) at stage II, the metabolite spectra of inoculated plants were similar to those of the control, and (ii) at stages IV and V, the leaf metabolic profiles of inoculated plants shifted towards the profiles of the control plants at earlier developmental stages. At stage IV the inoculated plants exhibited a higher level of metabolism of nitrogen, organic acids, and lipophilic compounds in comparison to control plants. Thus, mycorrhization led to the retardation of plant development, which was also associated with higher seed biomass accumulation in plants with an extended vegetation period. The symbiotic crosstalk between host plant and AM fungi leads to alterations in several biochemical pathways the details of which need to be elucidated in further studies.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Shtark

Puzanskiy

Avdeeva

et al. 2019

PeerJ

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“…Nonetheless, this accumulation does not always result in an increase of fresh weight. The low growth response of P. sativum to AMF mono-inoculation was shown in our previous research and some other publications [ 5 , 28 , 29 , 30 ]. Precise analysis of pea plant development during AM symbiosis revealed a very important event, namely that the pea plants inoculated with AMF prolonged the active phase of the vegetation period [ 5 ].…”

Section: Discussionsupporting

confidence: 64%

“…Some pea genotypes exhibit relatively high mycorrhiza-dependence [ 26 ]. However, most of the studies conducted under model conditions [ 5 , 10 , 27 , 28 , 29 , 30 ], and in the field [ 31 ], indicate that the pea has a low responsiveness to mono-inoculation with AMF. Often, a noticeable positive effect of mycorrhization on the growth parameters of pea plants is observed only when they are grown under stressful conditions [ 27 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Alterations in Pisum sativum Roots during Plant Growth and Arbuscular Mycorrhiza Development

Shtark

Puzanskiy

Avdeeva

et al. 2021

Plants

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Intensive exchange of nutrients is a crucial part of the complex interaction between a host plant and fungi within arbuscular mycorrhizal (AM) symbiosis. For the first time, the present study demonstrates how inoculation with AMF Rhizophagus irregularis affects the pea (Pisum sativum L.) root metabolism at key stages of plant development. These correspond to days 21 (vegetation), 42 (flowering initiation), and 56 (fruiting-green pod). Metabolome profiling was carried out by means of a state-of-the-art GC-MS technique. The content shifts revealed include lipophilic compounds, sugars, carboxylates, and amino acids. The metabolic alterations were principally dependent on the stage of plant development but were also affected by the development of AM fungi, a fact which highlights interaction between symbiotic partners. The comparison of the present data with the results of leaf metabolome profiling earlier obtained did not reveal common signatures of metabolic response to mycorrhization in leaves and roots. We supposed that the feedback for the development and symbiotic interaction on the part of the supraorganismic system (root + AM fungi) was the cause of the difference between the metabolic profile shift in leaf and root cells that our examination revealed. New investigations are required to expand our knowledge of metabolome plasticity of the whole organism and/or system of organisms, and such results might be put to use for the intensification of sustainable agriculture.

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“…Hence, involvement of high-EIBSM lines in breeding programs might increase the overall agricultural efficiency. One needs to keep in mind; however, that environmental stress, like drought, common in most pea culturing countries, might eliminate the favorable effects of symbiosis with BSM [57]. Therefore, additional experiments in adequate drought models [58] are necessary to address inoculation of high-EIBSM pea plants with BSM under conditions of environmental stress.…”

Section: Discussionmentioning

confidence: 99%

Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

et al. 2019

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Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.

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EVALUATION OF THE SYMBIOTIC EFFECTIVENESS OF PEA (Pisum sativum L.) GENOTYPES IN POT EXPERIMENT

Cited by 10 publications

References 12 publications

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Metabolic Alterations in Pisum sativum Roots during Plant Growth and Arbuscular Mycorrhiza Development

Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

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