Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation

Abstract

DDT (1,1,1-trichloro-2,2-(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, , and biosurfactant-producing bacteria, and , on DDT biodegradation. Bacteria were added into the culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 10 bacteria cells/ml culture). DDT was degraded to approximately 19% by during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of into culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2-(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by and. The results of this study indicate that has a synergistic relationship with and can be used to optimize the degradation of DDT by .

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

“…The DDT degradation by co-cultures of fungi and bacteria has been reported previously. The synergistic co-cultures P. aeruginosa -P. ostreatus [17], P. brevispora -B. subtilis [19], G. lingzhi -B. subtilis [21] were previously reported as having degradation efficiency of 75~86%. In the cases of brown-rot fungus, Sariwati et al [43] and Sariwati and Purnomo [44] reported that the addition of B. subtilis and P. aeruginosa increased DDT degradation by Fomitopsis pinicola, respectively.…”

“…It has the ability to weaken environmental contaminants such as polychlorinated dioxins [7], polychlorinated biphenyls [8], cyclodiene insecticide [9], industrial dye effluents, munitions waste, pesticides, herbicides, and petroleum hydrocarbons [10][11][12][13]. Some of the WRF that possess the capability to degrade DDT include Phanerochaete chrysosporium, Trametes versicolor [14], Pleurotus ostreatus [15][16][17], Pleurotus florida, Pleurotus sajorcaju, Pleurotus eryngii [18], Phlebia lindtneri and Phlebia brevispora [19,20], and Ganoderma lingzhi [21] as well as some brown-rot fungi [22][23][24]. Besides, Arisoy [18] also reported that P. eryngii could degrade DDT approximately 66% over a 20-day incubation period in mineral medium.…”

Microbial glycoconjugates in organic pollutant bioremediation: recent advances and applications

“…Bioaugmentation with the glycoconjugate-producing bacteria also proved to be an efficient technique for the remediation of pesticides. The α and β isomers of the endosulfan degraded by up to 82 % in the presence of glycocnjugates having biosurfactant properties [151,152].…”

Culture of Bacterium Bacillus subtilis as Degradation Agent in Attempt of Sea Water Remediation Contaminated By Petroleum

“…Besides, B. subtilis can be mixed with some brown-rot fungi (BRF), such as Gloephyllum trabeum, Daedalea dickinsii, and Fomitopsis pinicola as well as white-rot fungi (WRF) such as Pleurotus ostreatus, Pleurotus eryngii, and Ganoderma lingzhi. Mixed culture B. subtilis and BRF had been reported to enhance DDT degradation as well as dyes decolorization [29][30][31][32].…”