Evaluation of the therapeutic efficacy of albendazole-loaded silver nanoparticles against Echinococcus granulosus infection in experimental mice

Nassef, Nashaat E.; Saad, AbdelGawad; Harba, Nancy M.; Beshay, Engy; Gouda, Marwa A.; Shendi, S S; Mohamed, Asmaa S.

doi:10.1007/s12639-019-01145-z

Cited by 16 publications

(8 citation statements)

References 39 publications

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“…In addition, in the cysts in contact with this nanoparticle, there were marked ultrastructural changes. While measuring size, granuloma size, and weight of cysts, the greatest decrease was in ABZ loaded with silver nanoparticles [ 38 ] . In a study by Norouzi et al, the protoscolicidal effects of silver, silica, copper, iron, and zinc nanoparticles were evaluated.…”

Section: Discussionmentioning

confidence: 99%

High Potency of Organic and Inorganic Nanoparticles to Treat Cystic Echinococcosis: An Evidence-Based Review

et al. 2020

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Since there is no potential, effective vaccine available, treatment is the only controlling option against hydatid cyst or cystic echinococcosis (CE). This study was designed to systematically review the in vitro, in vivo, and ex vivo effects of nanoparticles against hydatid cyst. The study was carried out based on the 06- PRISMA guideline and registered in the CAMARADES-NC3Rs Preclinical Systematic Review and Meta-analysis Facility (SyRF) database. The search was performed in five English databases, including Scopus, PubMed, Web of Science, EMBASE, and Google Scholar without time limitation for publications around the world about the protoscolicdal effects of all the organic and inorganic nanoparticles without date limitation in order to identify all the published articles (in vitro, in vivo, and ex vivo). The searched words and terms were: “nanoparticles”, “hydatid cyst”, “protoscoleces”, “cystic echinococcosis”, “metal nanoparticles”, “organic nanoparticles”, “inorganic nanoparticles, “in vitro”, ex vivo”, “in vivo”. Out of 925 papers, 29 papers including 15 in vitro (51.7%), 6 in vivo (20.7%), ex vivo 2 (6.9%), and 6 in vitro/in vivo (20.7%) up to 2020 met the inclusion criteria for discussion in this systematic review. The results demonstrated the most widely used nanoparticles in the studies were metal nanoparticles such as selenium, silver, gold, zinc, copper, iron nanoparticles (n = 8, 28.6%), and metal oxide nanoparticles such as zinc oxide, titanium dioxide, cerium oxide, zirconium dioxide, and silicon dioxide (n = 8, 28.6%), followed by polymeric nanoparticles such as chitosan and chitosan-based nanoparticles (n = 7, 25.0%). The results of this review showed the high efficacy of a wide range of organic and inorganic NPs against CE, indicating that nanoparticles could be considered as an alternative and complementary resource for CE treatment. The results demonstrated that the most widely used nanoparticles for hydatid cyst treatment were metal nanoparticles and metal oxide nanoparticles, followed by polymeric nanoparticles. We found that the most compatible drugs with nanoparticles were albendazole, followed by praziquantel and flubendazole, indicating a deeper understanding about the synergistic effects of nanoparticles and the present anti-parasitic drugs for treating hydatid cysts. The important point about using these nanoparticles is their toxicity; therefore, cytotoxicity as well as acute and chronic toxicities of these nanoparticles should be considered in particular. As a limitation, in the present study, although most of the studies have been performed in vitro, more studies are needed to confirm the effect of these nanoparticles as well as their exact mechanisms in the hydatid cyst treatment, especially in animal models and clinical settings.

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Section: Discussionmentioning

confidence: 99%

High Potency of Organic and Inorganic Nanoparticles to Treat Cystic Echinococcosis: An Evidence-Based Review

et al. 2020

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“…Metal NPs and metal oxide NPs are the most widely utilized NPs for the treatment hydatid cyst followed by polymeric NPs 57 . Iron, silica, silver, copper, and zinc NPs 41 , gold NPs 42 , 52 , copper NPs 17 , silver NPs 38 , 58 , 59 , zinc oxide NPs 60 showed less protoscolicidal activities than PAMAM 61 . Malekifard et al 62 tested the effect of gold NPs on E. granulosus scolices and found that all concentrations of gold NPs had significant scolicidal effect and killed all protoscolices at 60 min at the concentration of 1 mg/ml.…”

Section: Discussionmentioning

confidence: 99%

In vitro and ex vivo protoscolicidal effect of poly(amidoamine) nanoemulsion against Echinococcus granulosus

Aboelsoued,

Toaleb,

Ibrahim

et al. 2024

Sci Rep

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Hydatidosis causes a serious health hazard to humans and animals leading to significant economic and veterinary and public health concern worldwide. The present study aimed to evaluate the in vitro and ex vivo protoscolicidal effects of synthesized poly(amidoamine), PAMAM, nanoemulsion. In this study, PAMAM was characterized through dynamic light scattering technique to investigate the particle size and zeta potential of nanoemulsified polymer. For the in vitro and ex vivo assays, we used eosin dye exclusion test and scanning electron microscope (SEM) to evaluate the effects of the prepared and characterized PAMAM nanoemulsion against protoscoleces from Echinococcus granulosus sensu lato G6 (GenBank: OQ443068.1) isolated from livers of naturally infected camels. Various concentrations (0.5, 1, 1.5 and 2 mg/mL) of PAMAM nanoemulsion at different exposure times (5, 10, 20 and 30 min) were tested against protoscolices. Our findings showed that PAMAM nanoemulsion had considerable concentration- and time-dependent protoscolicidal effect at both in vitro and ex vivo experiments. Regarding in vitro assay, PAMAM nanoemulsion had a potent protoscolicidal effect when compared with the control group with a highest protoscolicidal activity observed at the concentration of 2 mg/mL at all exposure times, such that 100% of protoscolices were killed after 20 min of exposure. Also, the mortality of protoscolices was 100% after 30 min of exposure to 1 and 1.5 mg/mL of PAMAM nanoemulsion, in vitro. Concerning ex vivo assay PAMAM nanoemulsion recorded the highest mortality rates at the concentration of 2 mg/mL (55, 99.4 and 100% at 10, 20, 30 min, respectively). Ultrastructure examination of examined protoscolices after 20 min of exposure to PAMAM nanoemulsion showed a complete loss of rostellar hooks, disruption of suckers with disorganization of hooks with partial or complete loss of them, and damage of protoscolices tegument with loss of their integrity in the form of holes and contraction of the soma region were observed in 1.5 and 2 mg/mL of PAMAM, in vitro and ex vivo, showing more damage in the in vitro conditions. It can be concluded that PAMAM nanoemulsion is a promising protoscolicidal agent offering a high protoscolicidal effect at a short exposure time. Further in vivo studies and preclinical animal trials are required to evaluate its efficacy and clinical applications against hydatid cysts.

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“…For example, they decrease the severity of necrosis, steatosis, and reduce the level of transaminase and IFN-γ. Combination therapy is associated with a greater degree of structural changes in echinococcal cysts (reduction of cyst size and cyst mass) [155,156].…”

Section: Mechanisms Of Antiparasitic Activitymentioning

confidence: 99%

Characterization and potential applications of silver nanoparticles: an insight on different mechanisms

Kodintcev

2022

Chim.Tech.Acta

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In the 21st century, a great interest is devoted to biomedical application of various nanoparticles, particularly, as a means of improving the effectiveness of therapy for different diseases. Silver nanoparticles (AgNPs) are the most studied and investigated type of nanoparticles. Due to the wide spectrum of their action, silver nanoparticles may be used both to influence pathogenic microorganisms and to improve the treatment of cancer. The basic physico-chemical characteristics and stabilizing agents play an important modifying role in the pharmacokinetics and pharmacodynamics of nanoparticles, determining the severity of the caused effect and their potential toxicity. This review summarizes the main physico-chemical properties of AgNPs and their impact on the biological effects. Additionally, biochemical and pathophysiological mechanisms of silver nanoparticles activity against various microorganisms and tumor cells are considered. Finally, we address the problems, associated with determining the optimal characteristics of nanoparticles, in order to increase their efficiency and reduce their toxicity for the macroorganism.

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Evaluation of the therapeutic efficacy of albendazole-loaded silver nanoparticles against Echinococcus granulosus infection in experimental mice

Cited by 16 publications

References 39 publications

High Potency of Organic and Inorganic Nanoparticles to Treat Cystic Echinococcosis: An Evidence-Based Review

High Potency of Organic and Inorganic Nanoparticles to Treat Cystic Echinococcosis: An Evidence-Based Review

In vitro and ex vivo protoscolicidal effect of poly(amidoamine) nanoemulsion against Echinococcus granulosus

Characterization and potential applications of silver nanoparticles: an insight on different mechanisms

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