Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells

Kukumberg, Marek; Yao, Yuan; Goh, S. H.; Neo, Dawn Jing Hui; Yao, Jia; Yim, Evelyn K.F.

doi:10.1002/adbi.201700217

Cited by 25 publications

(18 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyurethane nanotopographies and flat surfaces have also been shown to decrease the proliferation of HUVECs and human aortic cells (HAEC) [20]. The influence of various topographies on HUVECs was also recently confirmed using a MultiARChitecture (MARC) chamber showing that topography influences the adhesion and proliferation behavior significantly [35]. However, network formation was not investigated, which is crucial for tissue engineering approaches.…”

Section: Discussionmentioning

confidence: 99%

Topography-driven alterations in endothelial cell phenotype and contact guidance

Suarez

Ham

Brinker

et al. 2020

Heliyon

View full text Add to dashboard Cite

Understanding how endothelial cell phenotype is affected by topography could improve the design of new tools for tissue engineering as many tissue engineering approaches make use of topography-mediated cell stimulation. Therefore, we cultured human pulmonary microvascular endothelial cells (ECs) on a directional topographical gradient to screen the EC vascular-like network formation and alignment response to nano to microsized topographies. The cell response was evaluated by microscopy. We found that ECs formed unstable vascular-like networks that aggregated in the smaller topographies and flat parts whereas ECs themselves aligned on the larger topographies. Subsequently, we designed a mixed topography where we could explore the network formation and proliferative properties of these ECs by live imaging for three days. Vascular-like network formation continued to be unstable on the topography and were only produced on the flat areas and a fibronectin coating did not improve the network stability. However, an instructive adipose tissue-derived stromal cell (ASC) coating provided the correct environment to sustain the vascular-like networks, which were still affected by the topography underneath. It was concluded that large microsized topographies inhibit vascular endothelial network formation but not proliferation and flat and nano/microsized topographies allow formation of early networks that can be stabilized by using an ASC instructive layer.

show abstract

Section: Discussionmentioning

confidence: 99%

Topography-driven alterations in endothelial cell phenotype and contact guidance

Suarez

Ham

Brinker

et al. 2020

Heliyon

View full text Add to dashboard Cite

show abstract

“…Cell adhesion is critical in life systems, ranging from organisms to individual cells. It plays an important role in cell communication, cell regulation, organ formation, and tissue maintenance [1][2][3][4][5]. As a complex dynamic process, cell adhesion consists of adsorption of proteins to the surface and the expression of specific peptide sequences.…”

Section: Introductionmentioning

confidence: 99%

Recent advance in surface modification for regulating cell adhesion and behaviors

Cai

Yang

et al. 2020

Nanotechnology Reviews

417

247

View full text Add to dashboard Cite

Cell adhesion is a basic requirement for anchorage-dependent cells to survive on the matrix. It is the first step in a series of cell activities, such as cell diffusion, migration, proliferation, and differentiation. In vivo, cells are surrounded by extracellular matrix (ECM), whose physical and biochemical properties and micromorphology may affect and regulate the function and behavior of cells, causing cell reactions. Cell adhesion is also the basis of communication between cells and the external environment and plays an important role in tissue development. Therefore, the significance of studying cell adhesion in vitro has become increasingly prominent. For instance, in the field of tissue engineering and regenerative medicine, researchers have used artificial surfaces of different materials to simulate the properties of natural ECM, aiming to regulate the behavior of cell adhesion. Understanding the factors that affect cell behavior and how to control cell behavior, including cell adhesion, orientation, migration, and differentiation on artificial surfaces, is essential for materials and life sciences, such as advanced biomedical engineering and tissue engineering. This article reviews various factors affecting cell adhesion as well as the methods and materials often used in investigating cell adhesion.

show abstract

“…In 2018, Kukumberg et al 573 further developed this technology to create the MARC chamber and the MARC plate. The MARC chamber contained 42 distinct patterns (2 × 2 mm 2 ) mimicking the topographies on the MARC chip, with the added feature that it could robustly fit into conventional cell culture plates or dishes.…”

Section: Microarray Strategies Applied In Biomaterials Screening and Used To Model The Cellular Microenvironmentmentioning

confidence: 99%

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

View full text Add to dashboard Cite

The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

show abstract

Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells

Cited by 25 publications

References 75 publications

Topography-driven alterations in endothelial cell phenotype and contact guidance

Topography-driven alterations in endothelial cell phenotype and contact guidance

Recent advance in surface modification for regulating cell adhesion and behaviors

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

Contact Info

Product

Resources

About