Evaluation of the toxic influence of vitamin E (dl-alpha-tocopheryl acetate) and treatment with aqueous extracts of cinnamon or anise on lipid profile and liver functions of female wistar rats

Abstract

The toxic effects of vitamin E and its treatment with aqueous extracts of Cinnamon or anise on lipid profile and liver functions of female wistar rats were examined for six weeks during September 2016 at labs in al-Neelain University. 18 rats were divided into six groups: 1. negative control group (sunflower oil), 2. Positive control group and rest groups given (1500 mg/Kg/BW/day of Vitamin E). After two hours, the four treated groups received a low dose (2.13g/Kg) and a high dose (3.20g/Kg) 20g/Kg from Cinnamon aqueous extract (CAE) and Anise aqueous extract (AAE). At the end rats were sacrificed then serum and liver tissues were analyzed. Vitamin E toxic dose had caused a significant increase in serum Triglycerides (TG), Alanine amino transferase (ALT) levels, while it decreased the levels of Highdensity lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC) and Aspartate amino transferase (AST). All treatments decreased TG and ALT levels. CAE low dose significantly increased TC, LDL and HDL levels. CAE high dose caused a significant decrease in AST, TC, and LDL. Both doses of AAE, caused significant increases on AST levels, and only anise low dose caused a significant decrease on TC and LDL levels. Vitamin E toxic dose caused severe fatty change in liver histology, which was near normal in both doses of CAE with a small necrosis in a low dose. Only AAE low dose normalized the liver. To conclude Vitamin E oral administration with a dose of (1500 mg/Kg) induced liver injury with an elevation in ALT and TG levels, which was significantly ameliorated by both treatments. Cinnamon was better than anise in ameliorating the toxicity. Cinnamon high dose was better than Cinnamon low dose; in contrast anise low dose was better than Anise high dose.

Effect of Biscuits Supplemented with Cinnamon, Fennel, and Anise Powders on the Quality of Vital Functions in Aged Rats

“…(Nair et al, 2018) cleared that Pimpinella anisum L. have the ability to contribute to protection against renal failure by improving the structure of glomeruli and kidney tubules in the anisetreated group. ( Ibrahim, 2017( found that anise seeds have an improving effect on the functions of kidney enzymes, especially urea. This in agreement with our current study, the group treated with anise powder biscuits had a significant reduction in the level of urea compared to the control group at P< 0.05.…”

“…(Wu et al, 2018) showed that combined treatment with cinnamaldehyde and PTH enhances osteoblasts in a rat model of osteoporosis. (Ibrahim, 2017), reported that cinnamon extract helps increase sex hormones, including testosterone and improves testosterone level. (Azam et al, 2017) found that administering different concentrations of fennel seeds significantly increased the levels of estrogen and progesterone hormones in the blood of adult female rats.…”