Evaluation of the toxicity of Colocasia esculenta (Aracaceae): Preliminary study of leaves infected by Phytophthora colocasiae on wistar albinos rats

Nzebang, Doriane Camille Nyonseu; Njila, Marie Ide Ngaha; Bend, Emma F.; Oundoum, Pierre C. Oundoum; Landry, Koloko Brice; Zangueu, Calvin Bogning; Ekedi, Philippe Belle; Sameza, Modeste Lambert; Lembè, Dieudonné Massoma

doi:10.1016/j.biopha.2017.12.061

Cited by 9 publications

(4 citation statements)

References 7 publications

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“…The extract normalized the hematological values (WBC, RBC, HCT and MCV) depressed by ISO while significantly elevating the platelet count. The increased production of platelets suggests that the extract did not present a toxic effect, such as that of Colocasia esculenta (L.) Schott [38], but presented an increase in platelets similar to Carica papaya Linn. [39].…”

Section: Discussionmentioning

confidence: 99%

Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry

et al. 2019

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Guinep is traditionally used in the management of cardiovascular ailments. This study aims to evaluate its medicinal constituents and effects in the management of myocardial injury in an experimental isoproterenol (ISO) rat model. Sprague-Dawley rats were randomly assigned to four groups: Group 1 was the control group; Group 2 received M. bijugatus extract (100 mg/Kg; MB) for six weeks; Group 3 was given ISO (85 mg/Kg) i.p. twice during a 24-hour period; and Group 4 was given ISO (85 mg/Kg) i.p. and MB extract (100 mg/Kg) for six weeks. The MB was administered orally by gavage, daily. The blood pressure of conscious animals was measured, while ECG was performed under anesthesia. Blood and serum were collected for biochemical and hematological analysis. The ISO group treated with MB showed a significant decrease (p < 0.001) in (SBP), diastolic (DBP), mean arterial (MAP) and heart rate (HR) compared to the ISO only group. Conversely, MB treated rats that were not induced with ISO displayed a significant decreases (p < 0.001) in SBP, DBP, MAP, and HR. ISO significantly elevated the ST segment (p < 0.001) and shortened the QTc interval (p < 0.05), which were recovered after treatment with 100 mg/Kg of MB. In addition, the results showed a significant decrease (p < 0.001) in the heart to body weight ratio of the ISO group treated with MB compared to the ISO only group. Furthermore, the extract normalized the hematological values depressed by the ISO while significantly elevating the platelet count. UHPLC high-resolution orbitrap mass spectrometry analysis results revealed the presence of several antioxidants like vitamin C and related compounds, phenolic acids, flavonoid, fatty acids (oxylipins), and terpene derivatives. The results of this study indicated that Melicoccus bijugatus did display some cardio-protective effects in relation to myocardial injury.

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Section: Discussionmentioning

confidence: 99%

Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry

et al. 2019

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“…Biochemical markers in the blood provide valuable information on the effect of drug toxicity to the physiological status within the body [44]. In the subchronic toxicity test for CHB-II-F, only few biochemical markers were altered in the treatment groups compared to control.…”

Section: Discussionmentioning

confidence: 99%

Safety Evaluation of a New Traditional Chinese Medical Formula, Ciji-Hua’ai-Baosheng II Formula, in Adult Rodent Models

Zhai

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Background Ciji-Hua'ai-Baosheng II Formula (CHB-II-F) is a new traditional Chinese medical formula that has been shown to reduce toxicity and side effects of chemotherapy and increase the probability of cancer patient survival. Whether CHB-II-F is safe as an adjunctive therapy for cancer patients receiving chemotherapy has yet to be determined. Purpose To evaluate the acute and subchronic toxic effects of CHB-II-F in rodent models. Methods In acute toxicity test, 24 Kunming mice were divided into 2 groups: untreated control and CHB-II-F 1.05 g/mL (31.44 g/kg) treated group. Treatment was administered to the treated group 3 times a day for 14 days. The overall health, adverse reactions, and mortality rate were documented. In subchronic toxicity test, 96 Sprague-Dawley rats were divided into 4 groups: untreated control, high dose CHB-II-F (H) (26.20 g/kg), medium dose CHB-II-F (M) (13. 10 g/kg), and low dose CHB-II-F (L) (6.55 g/kg) [equal to 24.375 g (dried medicinal herb)/kg] treated groups. Treated groups were given the treatments once a day for 4 weeks. The overall health and mortality rate were recorded every day. Body weight and food consumption were measured once a week. Hematologic and biochemical parameters, organ weights, and histopathologic markers were analyzed after 4 weeks. An additional 2 weeks were given as the treatment recovery period before end-point euthanization, and biochemical analyses were performed. Results The maximum tolerated dose (MTD) of CHB-II-F on mice was found to be 94.31 g/kg [equal to 351 g (dried medicinal herb)/kg], which is 108 times the human adult dose. In the acute toxicity test, administration of CHB-II-F 31.44 g/kg showed no adverse effect and did not cause mortality. In the subchronic toxicity test, after 4 weeks of treatment, compared to the controls, total cholesterol (TCHO) level, cardiac and splenic indexes, body weights of female rats, and mean corpuscular hemoglobin concentration (MCHC) in the CHB-II-F (H) group were significantly increased; triglyceride (TG) in the CHB-II-F (M) group and liver and splenic indexes in the CHB-II-F (L) group were increased. After the two-week recovery period, biofluid analyses, food consumption, and histopathologic examinations showed no abnormalities. Conclusion Administration of CHB-II-F had no obvious adverse effect on the overall health of rodent models. A daily maximum dose of less than 94.31 g/kg or 6.55 g/kg CHB-II-F for 4 continuous weeks was considered safe.

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“…All the groups were treated once daily under the same housing conditions for a period of 28 days and daily animal weight measurement was ensured using a sensitive weighing scale (In tek, USA). The LD50 of AECEL has been estimated to be about 4000mg/kg by Nyonseu-Nzebang et al (2018) [12].…”

Section: Treatment Protocolmentioning

confidence: 99%

Ameliorative Effects of Aqueous Extract of Colocasia esculenta Leaf Against Lipopolysaccharide-Induced Prefrontal cortex damage in Mice

Siyanbade,

Iteire,

Adelakun

et al. 2024

Preprint

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Background The prefrontal-cortex (PFC) occupies about one-third of the total area of the cerebral cortex and it plays crucial role in Cognition and memory. This study evaluated the ameliorative effects of aqueous extract of Colocasia esculenta leaf (AECEL) on lipopolysaccharide-induced Prefrontal-cortex damage. Methods 42 Swiss male albino mice with weights ranging between 18 − 22g were randomized into six-groups. Group-A consisted of healthy-mice and LPS-induction in groups (B-F) was done by intraperitoneal injections of 0.5mg/kg of lipopolysaccharide (LPS) for seven days. Group-B received distilled-water; Group C- E was treated with AECEL at 400 mg/kg, 600 mg/kg and 800 mg/kg respectively, while Group F received 2.5 mg/kg of donepezil (DPZ) via oral-gavage for 28days. Results Significant increase in the brain oxidative-stress parameters was seen in the LPS-exposed groups compared to the control group (p < 0.05). However, compared with those in the LPS-only group, the levels of oxidative-stress parameters as well as Tumour-necrosis factor-α and interleukin-6 in the brain of AECEL-and DPZ-treated groups were significantly lower (p < 0.05). In the Y-maze test: the percentage-alternation (%ALTERN) significantly decreased (p < 0.05) in the LPS-only group compared to that in the control-group, but there was a significant increase in the %ALTERN in the AECEL-treated groups compared to that in the LPS-only and LPS + DPZ treatment groups (p < 0.05). Bielschowsky staining revealed that the LPS-only group exhibited senile-plaques and neurofibrillary-tangles. Conclusion LPS caused damage to the PFC; AECEL significantly improved cognitive function, memory, and anxiety-like behaviours.

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Evaluation of the toxicity of Colocasia esculenta (Aracaceae): Preliminary study of leaves infected by Phytophthora colocasiae on wistar albinos rats

Cited by 9 publications

References 7 publications

Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry

Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry

Safety Evaluation of a New Traditional Chinese Medical Formula, Ciji-Hua’ai-Baosheng II Formula, in Adult Rodent Models

Ameliorative Effects of Aqueous Extract of Colocasia esculenta Leaf Against Lipopolysaccharide-Induced Prefrontal cortex damage in Mice

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