Evaluation of the treatment with resveratrol-loaded nanoparticles in intestinal injury model caused by ischemia and reperfusion

Abstract

The gastrointestinal tract is extremely sensitive to ischemia and reperfusion (I/R). Studies have reported that resveratrol (RSV) is able to combat damage caused by intestinal I/R. Because of its effectiveness in increasing the permanence and bioavailability of resveratrol in the intestinal epithelium, we investigated whether the effect of resveratrol-loaded in poly(anhydride) nanoparticles reduce oxidative stress and promote myenteric neuroprotection in the ileum of rats subjected to I/R. Physicochemical evaluations were performed on nanoparticles. The animals were divided into nine groups (n = 6/group) and treated every 48 h. Treatments with resveratrol (7 mg/kg of body weight) were applied 5 days before surgery and continued for 7 days after surgery (reperfusion period). The superior mesenteric artery was occluded to cause I/R injury. Oxidative stress, myeloperoxidase, nitrite, aspartate aminotransferase, alanine aminotransferase, immunolabeling of myenteric neurons and glial cells, and gastrointestinal transit was evaluated. Both nanoparticle formulations presented negative charge with homogeneous distribution, and the payload, showed an encapsulation efficiency of 60%. Resveratrol administered in free form prevented alterations that were caused by I/R. The results of the groups treated with RSV-loaded nanoparticles presented similar results to the group treated with free resveratrol. Treatment with empty nanoparticles showed that poly(anhydride) is not an ideal nanocarrier for application in in vivo models of intestinal I/R injury, because of hepatotoxicity that may be caused by epithelial barrier dysfunction that triggers the translocation of nanoparticles.

Resveratrol modulates the gut microbiota to prevent murine colitis development through induction of Tregs and suppression of Th17 cells

“…68 In fact, this observation has led to a wealth of research focusing on how to increase the bioavailability of this potent anti-inflammatory natural product so that it can be absorbed and circulated to various affected organs, such as by way of encapsulation in nanoparticles or some other vehicle. 69,70 However, our findings suggest that resveratrol alters the microbiome directly and this leads to the anti-inflammatory effects during colitis, even before it becomes bioavailable to various organs after oral consumption.…”

“…It is particularly interesting to note that the poor bioavailability of resveratrol during oral consumption, which is attributed to the weak aqueous solubility of the compound, has always been an issue in terms to suggesting this natural product as a treatment of various disease . In fact, this observation has led to a wealth of research focusing on how to increase the bioavailability of this potent anti‐inflammatory natural product so that it can be absorbed and circulated to various affected organs, such as by way of encapsulation in nanoparticles or some other vehicle . However, our findings suggest that resveratrol alters the microbiome directly and this leads to the anti‐inflammatory effects during colitis, even before it becomes bioavailable to various organs after oral consumption.…”

Resveratrol Delivery by Albumin Nanoparticles Improved Neurological Function and Neuronal Damage in Transient Middle Cerebral Artery Occlusion Rats

“…Previous studies have highlighted that RES plays an important role in attenuating several kinds of I/R injury including cerebral I/R injury (Liu et al, 2015). However, its short in vivo half-life and low tissue affinity hampered the practical use of free RES (Nabavi et al, 2014; Borges et al, 2018). Therefore, a repeated administration of RES before and post the procedure of I/R was applied in most studies.…”

Remote organs respond differently to curcumin treatment after intestinal ischemia/reperfusion injury

“…The samples underwent the experimental protocols similarly to Borges et al (Borges et al, 2018). The samples were crushed and homogenized in 200 mM potassium phosphate Research, Society and Development, v. 9, n. 11, e1519119660, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i11.9660 buffer, pH 6.5.…”