Evaluation of the trisodium salt of 3-phosphoglycerate as a fuel for red cell storage

Guppy, Michael; Whisson, M E; Sabaratnam, R.; Niu, Xianwa; Britto, Joanne M.

doi:10.1007/bf02984525

Cited by 1 publication

(1 citation statement)

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“…Such a system would require an increase in the glucose concentration of the red cell additive, but this adjustment should pose no problems. Red cells show no deterioration for several days in the absence of glucose (Guppy et al, 1992) so that ample time would be available for the preparation of reconstituted red cells, even with delayed platelet preparation such as is used in the buffy coat method (Eriksson & Hogman, 199 1).…”

Section: Discussionmentioning

confidence: 99%

Quantitative study of starving platelets in a minimal medium: maintenance by acetate or plasma but not by glucose

Whisson¹,

Nakhoul²,

Howman³

et al. 1993

Transfusion Medicine

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The requirement of donor platelets for fuels, plasma and calcium were studied using platelets washed, filtered to remove leucocytes and resuspended in a new glucose-free minimal platelet storage medium with low citrate (3 mmol/l), low buffer capacity and no calcium. This is the first study of platelets stored without plasma, glucose or calcium and it was shown that platelets continued to aggregate with collagen plus adrenaline for 48 h and showed only a 50% fall in 'swirl index', an objective morphology score, after 3 days, showing that by these criteria human platelets do not require glucose. Sodium acetate extended the storage time by between 2 and 4 days, depending on the index parameter. This is the first evidence showing that failure of platelets in these conditions is at least partly due to exhaustion of fuel, and the first evidence that acetate prolongs in vitro survival. As little as 10% low-glucose plasma extended the storage time, but it was no better than acetate. New observations using this system included a very rapid fall in pH during resuspension of the washed platelet pellet, a rising pH in the absence of added fuel and an increased pH with added acetate.

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Section: Discussionmentioning

confidence: 99%