Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae

Lobová, Dana; Prášek, Josef; Čížek, Alois; Celer, V.

doi:10.1111/j.1472-765x.2011.03134.x

Cited by 2 publications

(2 citation statements)

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“…Even more problematic is the recent report that the gene encoding Bhlp29.7 could not be amplified from 33 (45%) recent German isolates of B. hyodysenteriae [14]. Although recently Lobova et al reported that Immunoblotting using Bhlp29.7 in conjunction with culturing method was a valuable tool for detecting swine herds latently infected with B. hyodysenteria [15], the specificity of the Bhlp29.7 is still not clear. Hence further work is required to identify alternative antigenic surface exposed proteins specific to B. hyodysenteriae that can be used in serological assays.…”

Section: Discussionmentioning

confidence: 99%

The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

2012

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BackgroundSwine dysentery (SD), a mucohaemorrhagic diarrhoeal disease of pigs, results from infection of the large intestine with the spirochaete Brachyspira hyodysenteriae. ELISA systems using whole spirochaete cells (WC) and the B. hyodysenteriae outer membrane lipoprotein Bhlp29.7 previously have been established as potential diagnostic tools for SD. However, their true value in identifying infected herds remains unclear. The present study aimed to compare the performance of whole-cell and Bhlp29.7 based ELISAs in detecting specific immunoglobulin class IgG and IgM to B. hyodysenteriae in growing pigs, and additionally evaluated whether meat juice could serve as a source of specific antibodies.ResultsLevels of circulating IgG and IgM reacting with WC spirochaete preparations and recombinant Bhlp29.7 peaked 4-6 weeks post-infection in the experimentally challenged pigs, and remained elevated in the present study. In a cohort of pigs on an infected farm levels of antibody directed against both antigens showed a progressive increase with time. However, other than for the level of IgG against WC antigen, a significant increase in antibody levels also was observed in a cohort of pigs on a non-infected farm. In addition, assays using meat juice had 100% specificity and equivalent sensitivity to those based on serum, and likewise the best performance was achieved using the WC IgG ELISA.ConclusionsIgG ELISAs using either WC or Bhlp29.7 as plate-coating antigens were shown to be useful for monitoring the dynamics of B. hyodysenteriae infection in grower pigs. Of the two antigens, the WC preparation tended to give better discrimination between pigs from infected and non-infected farms. Testing of meat juice was shown to have potential for identifying infected herds.

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Section: Discussionmentioning

confidence: 99%

The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

2012

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“…En la República Checa, se describió una prevalencia de la infección del 27,3 % en un estudio que incluyó 22 granjas con y sin antecedentes de disentería, y 880 muestras de heces de animales con o sin historial o sospecha de DP, entre 1997 y 2006 (Lobova et al 2011).…”

Section:  República Checaunclassified

Aportaciones al diagnóstico y control de la disentería porcina = Contributions to the dianosis and control of swine dysentery

Morán¹,

Miguel²

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Las espiroquetas son un grupo de bacterias que forman el filo Spirochaetes, uno de los 25 filos en que se divide actualmente el superreino Bacteria. Forman un amplio grupo bacteriano filogenéticamente relacionado, con evidencias fenotípicas comunes como es la presencia de flagelos periplasmáticos y otras características como la resistencia de la mayoría de sus miembros a la rifampicina (Kunkle & Kinyon 1988; Wyss et al. 1996; Stamm et al. 2001). El filo Spirochaetes contiene una única clase, Spirochaetia, y seis ordenes, entre ellos el orden Brachyspirales, formado por una única familia, Brachyspiraceae, con un único género denominado Brachyspira, en el que están incluidas las espiroquetas intestinales con mayor relevancia clínica para especies como el cerdo, como veremos más adelante. Clásicamente, los actuales ordenes se clasificaban como familias dentro del orden Spirochaetales, distinguiéndose entre ellas en base a sus características bioquímicas y a las secuencias del ácido ribonucleico ribosómico (ARNr) 16S o del gen que lo codifica (Paster et al. 1991; Paster & Dewhirst 2000). Estudios recientes, basados en evidencias filogenéticas y moleculares, propusieron que dichas familias podían ser, en realidad, divisiones taxonómicas mayores como órdenes o incluso clases dentro del propio filo Spirochaetes, como actualmente están clasificadas (Gupta et al. 2013). Dentro del filo Spirochaetes se incluyen cuatro géneros con gran importancia clínica que engloban especies causantes de importantes enfermedades. Así, Treponema pallidum es el agente etiológico de la sífilis, Borrellia burgdorferi causa la enfermedad de Lyme y especies de los géneros Leptospira y Brachyspira causan leptopirosis y espiroquetosis intestinales, respectivamente, en diversas especies animales incluido el hombre (Gupta et al. 2013).

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Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae

Abstract: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease.

Cited by 2 publications

References 24 publications

The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

Aportaciones al diagnóstico y control de la disentería porcina = Contributions to the dianosis and control of swine dysentery

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