Evaluation of therapeutic effects of teriparatide in a rat model of zoledronic acid-induced bisphosphonate-related osteonecrosis

Ikeda, Hisazumi; Yoshiga, Daigo; Kokabu, Shoichiro; Ariyoshi, Wataru; Tsurushima, Hiroki; Sakaguchi, Osamu; Tanaka, Jumpei; Kaneko, Junya; Habu, Manabu; Sasaguri, Masaaki; Jimi, Eijiro; Nishihara, Tatsuji; Yoshioka, Izumi; Tominaga, Kazuhiro

doi:10.1016/j.ajoms.2019.03.001

Cited by 2 publications

(1 citation statement)

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“…However, long-term administration and high ZA doses often lead to osteonecrosis of the jaw [ 8 , 9 ]. Di Vito et al, in 2020 [ 10 ], evaluated the biological effects of different ZA concentrations on the in vitro model of periodontal ligament stem cells.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Low Amounts of Zoledronic Acid by HPLC-ESI-MS Analysis: Method Development and Validation

Petrovici¹,

Silion²,

Simiónescu³

et al. 2022

IJMS

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Zoledronic acid (ZA) is used in the treatment of various bone pathologies, but it forms complexes with calcium ions present in body fluids, decreasing ZA bioavailability. Thereby, the study first describes the identification of ZA-calcium complexes that form in calcium-rich environments, in order to establish the bioavailable ZA concentration. Then, a new method for quantification of low ZA amounts in milieus that mimics in vivo conditions by using simulated body fluid and calcium sulfate hemihydrate was described. Almost all analytical methods of ZA quantification described in the literature require compound derivatization. At very low concentrations, derivatization is prone to analyte loss, therefore compromising the analytical results. In our study, we avoided ZA derivatization by using a high-performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS) system, conducting the investigation based on the fragmentation mass extracted ion chromatograms specific to the ZA protonated form. The method was validated by selectivity, precision, accuracy, linearity, signal to noise ratio, and limit of detection and limit of quantification calculation. Experimentally, this method can detect ranges of 0.1–0.5 ng/mL and precisely quantify ZA concentrations as low as 0.1 ng/mL. This method could provide the basis for quantifying low amounts of ZA in the blood during long-term administration.

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Section: Introductionmentioning

confidence: 99%