Evaluation of Thiol Homeostasis in Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorders

Arslan, Burak; Arslan, Gökçe Ayhan; Tuncer, Aslı; Karabudak, Rana; Dinçel, Aylin Sepici

doi:10.3389/fneur.2021.716195

Cited by 14 publications

(16 citation statements)

References 61 publications

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“…A combination of agents that modify the course of the disease and have various effects on immunity is used for treatment at different phases of the disease. 4,5 Vitamin plus interferon therapy and the use of medical cannabis have also been studied as methods of treatment in MS. 6,7 This disease leads to a widespread demyelination caused by selective involvement of oligodendrocytes and axonal loss. Additional psychobehavioral symptoms such as anxiety, dementia and depression are also observed.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of different intensity of aquatic exercise in enhancing remyelination and neuronal plasticity using cuprizone model in male Wistar rats

Begum¹,

Subramanian²,

Raghunath³

et al. 2022

Adv Clin Exp Med

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Background. Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system (CNS). Most exercise studies concentrate on the impact of exercise on cardiovascular system; this study aims to present the effects of exercise of varying intensity on the nervous system. Most recently in MS, positive outcomes were obtained with resistance and high-intensity exercises. This study also analyzes the effects of a prior conditioning program before the induction of demyelination and subsequent neuroprotective effects of such program. Objectives.To study and determine the neuroprotective and remyelinating effects of different intensity of aquatic exercise and a preconditioning exercise program on demyelination induced by oral administration of cuprizone (Cup). Materials and methods.Six groups of animals, each containing 6 rats, were used in the study. The groups were as follows: group I -control group; group II -Cup group; group III -treated with methylprednisolone (MP); group IV -treated with low-intensity exercise (LIE), free swimming for 40 min and high-intensity exercise (HIE); group V -treated with a resistance of 9% body weight and free swimming for 40 min; group VI -treated with preconditioning exercise (free swimming for 40 min for 3 weeks) before Cup administration followed by the same exercise protocol as for group V. All data were analyzed using one-way analysis of variance (ANOVA) with Tukey's test, by means of SigmaPlot v. 14.5 software. Results.Similarly to the MP group, group VI showed a positive outcome. A value of p < 0.001 was considered statistically significant. Also, group VI showed improved areas of remyelination in histopathology, an increased expression of myelin basic protein (MBP), reduced expression of glial fibrillary acidic protein (GFAP) in corpus callosum, and improved gene expression of brain-derived neurotrophic factor (BDNF) in the hippocampus region.Conclusions. General fitness achieved through a preconditioning program combined with HIE showed neuroprotective effects, as evidenced by increased areas of remyelination and improved neuronal plasticity, observed mostly in group VI (conditioning+HIE).

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Section: Introductionmentioning

confidence: 99%

Efficacy of different intensity of aquatic exercise in enhancing remyelination and neuronal plasticity using cuprizone model in male Wistar rats

Begum¹,

Subramanian²,

Raghunath³

et al. 2022

Adv Clin Exp Med

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“…However, the methods applied and the results are quite divergent and data concerning the ratio of thiols and disulfides are scarce. Nanomolar [ 21 ] and micromolar concentrations [ 22 , 23 ] of GSH in CSF have been reported. In some cases, the reduced form predominates in accordance with the high fraction of HMA.…”

Section: Discussionmentioning

confidence: 99%

“…In some cases, the reduced form predominates in accordance with the high fraction of HMA. Another study reports higher disulfides compared to thiols [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study

Paar

Seifried

Cvirn

et al. 2022

IJMS

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Like in many other pathologies, oxidative stress is involved in the development of neurodegenerative disorders. Human serum albumin (HSA) is the main protein in different body fluids including cerebrospinal fluid (CSF). By its redox state in terms of cysteine-34, albumin serves as marker for oxidative burden. We aimed to evaluate the redox state of HSA in patients with multiple sclerosis in serum and CSF in comparison to controls to identify possible correlations with disease activity and severity. Samples were stored at −70 °C until analysis by HPLC for the determination of albumin redox state in terms of the fractions of human mercaptalbumin (HMA), human nonmercaptalbumin1 (HNA1), and human nonmercaptalbumin2 (HNA2). Albumin in CSF showed significantly higher fractions of the reduced form HMA and decreased HNA1 and HNA2. There was no difference between albumin redox states in serum of patients and controls. In CSF of patients HNA2 showed a trend to higher fractions compared to controls. Albumin redox state in serum was associated with physical disability in remission while albumin redox state in CSF was related to disease activity. Thus, albumin redox state in serum and CSF of patients in relation to disease condition merits further investigation.

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“…Even though the presentation of the disease is mostly relapsing-remitting (85%), ~15% of it is progressive from the onset. Both oxidative stress and neuroinflammation play a pivotal role in the pathophysiology of MS. As a result of these pathological events, axonal damage is unavoidable in the end [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cerebrospinal fluid neurofilament light chain levels in multiple sclerosis and non-demyelinating diseases of the central nervous system: clinical and biochemical perspective

Arslan

Tuncer

et al. 2022

Bosn J of Basic Med Sci

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The neurofilament light chain (NfL) is a promising biomarker in the diagnosis, prognosis, and treatment response evaluation of neurological diseases. The aims of this study were to compare the cerebrospinal fluid (CSF) NfL levels in multiple sclerosis (MS) and certain non-demyelinating diseases of the central nervous system (NDCNS); to determine the relationship between clinical and radiological features and CSF NfL levels in patients with MS; and to compare the enzyme-linked immunosorbent assay (ELISA) and single molecule array (SIMOA) methods for NfL measurement using paired CSF and serum samples. We retrospectively analyzed the clinical data and performed NfL measurements in CSF and serum samples of newly diagnosed and treatment-naive patients with CNS diseases evaluated between 1 January 2019 and 1 January 2020. Eligible patients were divided into three groups: MS (n=23), differential diagnosis of MS (n=19), and NDCNS (n=42). First, we compared the CSF NfL levels among the three groups using the previously validated CSF ELISA assay. Next, we evaluated the relationship between CSF NfL levels and the clinical and radiological findings in MS group. Finally, we compared CSF and serum samples from patients of the MS groups (paired serum and CSF samples, n=19) using two different methods (ELISA and SIMOA). The CSF NfL level was the highest in the NDCNS group (1169.64 [535.92-5120.11] pg/mL, p=0.025). There was a strong positive correlation between the number of T2 lesions and CSF NfL level (r=0.786, p<0.001) in the MS group. There was excellent consistency between ELISA and SIMOA for CSF samples, but not for serum samples. Our results indicated that CSF NfL levels may also be used in the management of NDCNS and that SIMOA is the most reliable method for serum NfL determination.

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Evaluation of Thiol Homeostasis in Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorders

Cited by 14 publications

References 61 publications

Efficacy of different intensity of aquatic exercise in enhancing remyelination and neuronal plasticity using cuprizone model in male Wistar rats

Efficacy of different intensity of aquatic exercise in enhancing remyelination and neuronal plasticity using cuprizone model in male Wistar rats

Redox State of Human Serum Albumin in Multiple Sclerosis: A Pilot Study

Evaluation of cerebrospinal fluid neurofilament light chain levels in multiple sclerosis and non-demyelinating diseases of the central nervous system: clinical and biochemical perspective

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