Evaluation of three immunological tests for the diagnosis of pulmonary tuberculosis in a rural endemic area of Bangladesh

Islam, Mohammad Tariqul; Rabbi, Fazle; Ferdous, Shameema; Parvin, Ummey Shahnaz; Hossain, Akram; Hossain, Mohammad Sorowar

doi:10.1016/j.ijmyco.2014.03.003

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…In this study, admitted patients were tested with Mantoux test and 95% had MT positive (Figure-I) and found highly sensitive though it has false positive and false negative with some issues and cannot confirm diagnosis, it helped making initial screening and a route to diagnosis. In Islam MT Mantoux test was found more sensitive tool and 96% was found positive for TB 21 . In another study by Singh S, it was found similar result of MT and was 94.3% positive for TB and was highly sensitive 22 .…”

Section: Discussionmentioning

confidence: 89%

Leishmaniasis and Tuberculosis Concurrent Infection in Humans and Animals in Kala-azar Endemic Areas of Bangladesh

Rahman

Alam

Sarker³

et al. 2020

J. Armed Forces Med. Coll.

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Introduction: Co-infection of parasitic disease and pulmonary tuberculosis are increasing public health problem especially in developing countries like Bangladesh. More than 81% of cases and deaths from TB are in developing countries and is aggravated by concurrency with parasitic diseases, where in Bangladesh suffer a substantial no of Kala-azar cases in each year. Cattle are found to have bovine tuberculosis but at the same areas of Kala-azar endemicity, whether this animal found to be positive. Objectives: To findout the concurrent infection in humans and animals in Kala-azar endemic areas of Bangladesh. Materials and Methods: This study was conducted at Surya Kanto Hospital, Mymensingh and four Kala-azar endemic Upazilla of Mymensingh, Tangail and Pabna District. A total of 300 highly suspected tuberculosis patients reported from Kala-azar endemic areas and 170 cattle’s blood sample, 110 cattle spleen samples were included in this study. Cattle blood and spleen samples were investigated for both TB and Kalaazar and all the TB cases were investigated for Kala-azar. Buffy coat from venous blood was taken for ELISA and PCR. Spleen samples were sonicated and then were examined by PCR. All patients’ blood was tested with ICT (rK39) for Visceral Leishmaniasis. ICT positive patient’s splenic aspiration was examined by smear microscopy with 10x 100 magnifications. After that, all these samples were tested by ELISA and PCR. Results: Out of total 300 patients 162(54%) were found positive for TB. Blood sample of 180 patients was tested with ICT rK39 and 12(7%) patients were found positive for leishmaniasis who suffered from Tuberculosis. These 12(7%) patient’s splenic smear were examined of which 11(92%) of them were found positive for leishmania. But using ELISA all 12(100%) patient smears were found positive. Out of 170 cattle blood 12(7.1%) were ICT positive indicating prevalence of bovine tuberculosis and of these 170 cattle blood 20(11.8%) were found positive for antileishmania antibody. With PCR of these 20, no one was found positive for Leishmania. Conclusion: Visceral leishmaniasis and tuberculosis coinfection have drawn attention clinically. This study found the presence of leishmaniasis and tuberculosis concurrently in humans in Kala-azar endemic areas, wherein bTB antibody was detected in cattle. Though concurrently leishmania antibody was found in cattle but was not proved by PCR which requires further studies. Journal of Armed Forces Medical College Bangladesh Vol.14 (2) 2018: 91-96

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Section: Discussionmentioning

confidence: 89%

Leishmaniasis and Tuberculosis Concurrent Infection in Humans and Animals in Kala-azar Endemic Areas of Bangladesh

Rahman

Alam

Sarker³

et al. 2020

J. Armed Forces Med. Coll.

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“…Additionally, a test able to diagnose LTBI or incipient active TB (iATB) would greatly improve early diagnosis, assist with patient management programs, reduce disease dissemination and the current socioeconomic disease burden (16)(17)(18). There is therefore clearly an urgent need for the development of rapid, inexpensive and accurate tests for diagnosis of TB particularly in the point of care (POC) and remote settings (19)(20)(21)(22)(23). A biomarker protein-based, non-sputum diagnostic test such as an LFD would fulfil these criteria.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Host Protein Biomarkers by ELISA From Whole Lysed Peripheral Blood for Development of Diagnostic Tests for Active Tuberculosis

Garlant¹,

Kalaiarasan

Hewitt³

et al. 2022

Front. Immunol.

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Tuberculosis (TB) remains a significant global health crisis and the number one cause of death for an infectious disease. The health consequences in high-burden countries are significant. Barriers to TB control and eradication are in part caused by difficulties in diagnosis. Improvements in diagnosis are required for organisations like the World Health Organisation (WHO) to meet their ambitious target of reducing the incidence of TB by 50% by the year 2025, which has become hard to reach due to the COVID-19 pandemic. Development of new tests for TB are key priorities of the WHO, as defined in their 2014 report for target product profiles (TPPs). Rapid triage and biomarker-based confirmatory tests would greatly enhance the diagnostic capability for identifying and diagnosing TB-infected individuals. Protein-based test methods e.g. lateral flow devices (LFDs) have a significant advantage over other technologies with regard to assay turnaround time (minutes as opposed to hours) field-ability, ease of use by relatively untrained staff and without the need for supporting laboratory infrastructure. Here we evaluate the diagnostic performance of nine biomarkers from our previously published biomarker qPCR validation study; CALCOCO2, CD274, CD52, GBP1, IFIT3, IFITM3, SAMD9L, SNX10 and TMEM49, as protein targets assayed by ELISA. This preliminary evaluation study was conducted to quantify the level of biomarker protein expression across latent, extra-pulmonary or pulmonary TB groups and negative controls, collected across the UK and India, in whole lysed blood samples (WLB). We also investigated associative correlations between the biomarkers and assessed their suitability for ongoing diagnostic test development, using receiver operating characteristic/area under the curve (ROC) analyses, singly and in panel combinations. The top performing single biomarkers for pulmonary TB versus controls were CALCOCO2, SAMD9L, GBP1, IFITM3, IFIT3 and SNX10. TMEM49 was also significantly differentially expressed but downregulated in TB groups. CD52 expression was not highly differentially expressed across most of the groups but may provide additional patient stratification information and some limited use for incipient latent TB infection. These show therefore great potential for diagnostic test development either in minimal configuration panels for rapid triage or more complex formulations to capture the diversity of disease presentations.

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Evaluation of three immunological tests for the diagnosis of pulmonary tuberculosis in a rural endemic area of Bangladesh

Abstract: Considering the resource-constraints of rural and remote areas, the Mantoux test could be an alternative tool for the diagnosis of active PTB.

Cited by 2 publications

References 16 publications

Leishmaniasis and Tuberculosis Concurrent Infection in Humans and Animals in Kala-azar Endemic Areas of Bangladesh

Leishmaniasis and Tuberculosis Concurrent Infection in Humans and Animals in Kala-azar Endemic Areas of Bangladesh

Evaluation of Host Protein Biomarkers by ELISA From Whole Lysed Peripheral Blood for Development of Diagnostic Tests for Active Tuberculosis

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