Evaluation of Tissue Culture and Growth Assays to Identify Irradiated from Non-Irradiated Vegetables

Al-Safadi, B.; Sharabi, N.; Nabulsi, I.

doi:10.1300/j068v08n01_12

Cited by 5 publications

(1 citation statement)

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“…Therefore, tissue culture techniques may be very useful for breeding programs. Moreover, radiation treatments may increase the somaclonal variation and it was possible to select useful altered plants in vitro (Suciu et al, 1991;Croci et al,1990;Al-Safadi et al,2002). The present investigation aimed to induce maximum variation in garlic plants through callus irradiation to increase genetic variability in garlic plants derived from tissue culture technique to select the suitable one (s).…”

Section: Introductionmentioning

confidence: 96%

Effect of Gamma Rays on Callus Growth and Plantlet Regeneration in Garlic

Metwally¹,

El-Denary²,

Kasem³

2015

Journal of Plant Production

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This study was carried out in the Tissue Culture Laboratory, Horticulture Department, Faculty of Agriculture, Kafr El-Sheikh University in 2014 for increasing genetic variability plants. Fresh callus (21 days old) derived from root tips segments from Balady cv. was exposed to nine doses of gamma rays (0, 50, 100, 150, 200, 250, 500, 1000 and 2000 rad). After 21 days from radiation treatments, low doses of gamma rays stimulated callus growth, while higher doses decreased them. Low doses of gamma rays (50 rad) produced the highest callus fresh weight, while, 2000 rad produced the lowest value. After 60 days from radiation treatments, the effect of radiation was continuously noticed. Callus fresh weight was increased with increasing the radiation dose from 50 to 100 rad, and then decreased with increasing gamma irradiation doses. Therefore the lowest value of callus fresh weight was recorded at 2000 rad. Gamma rays at 100 rad produced the lowest differentiated plantlets per callus (normal, abnormal and total). Callus produced 100% normal plantlets at the low doses of radiation, i.e., from 50 to 250 rad, however some of abnormal plantlets were produced at the higher doses of gamma rays. Percentage of abnormal plantlets increased by increasing gamma ray doses until 500 rad which produced the highest record (18.1%).

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Section: Introductionmentioning

confidence: 96%