Evaluation of tissue metal and trace element content in a rat model of non-alcoholic fatty liver disease using ICP-DRC-MS

Gatiatulina, Eugenia R.; Popova, Elizaveta V.; Polyakova, Valentina S.; Skalnaya, Anastasia A.; Agletdinov, Eduard F.; Nikonorov, Alexandr А.; Skalny, Anatoly V.; Tinkov, Alexey A.

doi:10.1016/j.jtemb.2016.08.007

Cited by 21 publications

(14 citation statements)

References 62 publications

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“…The fat content in the diet did not affect the serum and tissular Fe levels. These results are similar to other research findings, where diminished hepatic [45,46] and renal [47] Zn concentrations were observed. Similarly, the renal Cu content decreased in the rats fed a high-fat diet [48], those fed a high-fat/ high fructose diet [47] and in the rats with non-alcoholic fatty liver disease (NAFLD) [46].…”

Section: Discussionsupporting

confidence: 93%

“…Other authors also observed reduced serum Cu concentration [ 45 ]. The high-fat diet did not affect the Fe content in laboratory animals’ liver [ 45 – 47 ], kidney [ 47 , 48 ] and serum [ 45 , 46 , 48 ]. The changes in the mineral status may have been caused by the fact that the rats fed high-fat diets had lower absorption and/or lower intake of these minerals.…”

Section: Discussionmentioning

confidence: 99%

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The Influence of Taurine Supplementation on Serum and Tissular Fe, Zn and Cu Levels in Normal and Diet-Induced Insulin-Resistant Rats

Król

Okulicz

Kupsz

2020

Biol Trace Elem Res

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Taurine (Tau) is a β-sulphonated amino acid postulated to improve glucose homeostasis in insulin resistance and diabetes. Changes in carbohydrate metabolism are accompanied by oxidative stress, which may disturb the mineral balance. Therefore, the aim of this study was to assess the effect of Tau supplementation on the levels of trace elements in rats fed either a standard (AIN-93M, 4% fat) diet or a modified high-fat diet (30% fat). For 8 weeks, male Wistar rats were fed these diets supplemented with 3% Tau. Taurine supplementation normalized increased serum insulin concentration and insulin resistance index; however, it did not improve serum CRP concentration in high-fat diet fed rats. The high-fat diet supplemented with Tau decreased the renal and splenic Zn levels, but the tissular Fe content did not change. The effect of Tau supplementation on the mineral balance to some extent depended on the fat content in the rats' diet. The high-fat diet supplemented with Tau decreased the rats' splenic Zn levels but increased their femur levels. In the group fed the standard diet, Tau reduced the rats' femur Zn level, whereas their splenic Zn level was comparable. Tau supplementation decreased the renal Cu level and serum ceruloplasmin concentration in the rats fed the standard diet, but this effect was not observed in the rats fed the high-fat diet. In conclusion, supplementary taurine failed to ameliorate disturbances in mineral homeostasis caused by high-fat diet feeding and led to tissular redistribution of Zn and Cu in the rat.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The Influence of Taurine Supplementation on Serum and Tissular Fe, Zn and Cu Levels in Normal and Diet-Induced Insulin-Resistant Rats

Król

Okulicz

Kupsz

2020

Biol Trace Elem Res

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“…Relative reductions in liver contents of fallout plutonium in humans with liver diseases have also been reported [24,25]. In experimental studies, reductions in liver tissue metals and trace elements have been reported in a model of non-alcoholic fatty liver disease and these reductions were often concomitant with decreases in total liver protein [26]. Various liver diseases and at various stages of disease may also alter biliary excretion of the plutonium and thus fecal excretion rates relative to urinary excretion.…”

Section: Discussionmentioning

confidence: 88%

The effects of chronic diseases on plutonium urinary excretion in former workers of the Mayak Production Association

et al. 2020

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The radiochemical analysis of plutonium activity in urine is the main method for indirect estimation of doses of internal exposure from plutonium incorporation in professional workers. It was previously shown that late-in-life acute diseases, particularly those that affect the liver, can promote accelerated rates of release of plutonium from the liver with enhanced excretion rates. This initial study examines the relationships of some chronic diseases on plutonium excretion as well as the terminal relative distribution of plutonium between the liver and skeleton. Fourteen cases from former workers at the Mayak Production Association (Mayak PA) who provided from 4–9 urine plutonium bioassays for plutonium, had an autopsy conducted after death, and had sufficient clinical records to document their health status were used in this study. Enhanced plutonium excretion was associated with more serious chronic diseases, including cardiovascular diseases and other diseases that involved the liver. These chronic diseases were also associated with relatively less plutonium found in the liver relative to the skeleton determined by analyses conducted after autopsy. These data further document health conditions that affect plutonium biokinetics and organ deposition and retention patterns and suggest that health status should be considered when conducting plutonium bioassays as these may alter subsequent dosimetry and risk models.

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“…Consequently, automated image segmentation methods are useful when identifying different regions, and for follow-up tasks based on image classification and lesion area identification analysis for improved diagnosis accuracy [28] [29] [30]. However, mice liver tissues are different from other tissues.…”

Section: Introductionmentioning

confidence: 99%

Morphological Segmentation Analysis and Texture-based Support Vector Machines Classification on Mice Liver Fibrosis Microscopic Images

Wang

Shi

Cao

et al. 2019

CBIO

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Background: To reduce the intensity of the work of doctors, pre-classification work needs to be issued. In this paper, a novel and related liver microscopic image classification analysis method is proposed. Objective: For quantitative analysis, segmentation is carried out to extract the quantitative information of special organisms in the image for further diagnosis, lesion localization, learning and treating anatomical abnormalities and computer-guided surgery. </P><P> Methods: In the current work, entropy-based features of microscopic fibrosis mice’ liver images were analyzed using fuzzy c-cluster, k-means and watershed algorithms based on distance transformations and gradient. A morphological segmentation based on a local threshold was deployed to determine the fibrosis areas of images. Results: The segmented target region using the proposed method achieved high effective microscopy fibrosis images segmenting of mice liver in terms of the running time, dice ratio and precision. The image classification experiments were conducted using Gray Level Co-occurrence Matrix (GLCM). The best classification model derived from the established characteristics was GLCM which performed the highest accuracy of classification using a developed Support Vector Machine (SVM). The training model using 11 features was found to be accurate when only trained by 8 GLCMs. Conclusion: The research illustrated that the proposed method is a new feasible research approach for microscopy mice liver image segmentation and classification using intelligent image analysis techniques. It is also reported that the average computational time of the proposed approach was only 2.335 seconds, which outperformed other segmentation algorithms with 0.8125 dice ratio and 0.5253 precision.</P>

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Evaluation of tissue metal and trace element content in a rat model of non-alcoholic fatty liver disease using ICP-DRC-MS

Cited by 21 publications

References 62 publications

The Influence of Taurine Supplementation on Serum and Tissular Fe, Zn and Cu Levels in Normal and Diet-Induced Insulin-Resistant Rats

The Influence of Taurine Supplementation on Serum and Tissular Fe, Zn and Cu Levels in Normal and Diet-Induced Insulin-Resistant Rats

The effects of chronic diseases on plutonium urinary excretion in former workers of the Mayak Production Association

Morphological Segmentation Analysis and Texture-based Support Vector Machines Classification on Mice Liver Fibrosis Microscopic Images

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