Evaluation of Tocopherol Recovery Through Simulation of Molecular Distillation Process

a-Tocopherol, c-tocopherol and d-tocopherol were successfully separated and purified from soybean vitamin E concentrate (53% purity, from soybean oil deodorizer distillate) using a low-pressure glass column (500 mm 9 25 mm, I.D., packed with silica gel). The effects of eluent, flow-rate and sample loading on separation efficiency were investigated using product purity and recovery as evaluation indices. On the basis of the single factor experiment results, an orthogonal test was designed to optimize the chromatographic separation conditions. The optimum conditions obtained by using analyses of extreme difference and variance are as follows: cyclohexane-ethanol 99.7:0.3 (v/v), flow-rate at 25 ml/min and loading amount being 2 ml (concentration 1 g/ml). Under these optimum conditions, purity of the a-tocopherol, c-tocopherol and d-tocopherol were 92.35, 91.23, 89.95%, respectively; the recovery of those products were 35.21, 36.25, 61.25%, respectively. The advantage of this process is that high purity individual tocopherols can be obtained directly from soybean vitamin E concentrate at 53% purity, without additional purification steps.

Section: Introductionmentioning

confidence: 99%

Separation of Individual Tocopherols from Soybean Distillate by Low Pressure Column Chromatography

Wan

Zhang

Jiang

et al. 2008

“…Molecular distillation is suitable for separation and purification of reaction mixtures with a high boiling point, high viscosity and thermally unstable materials, and it has been widely used in food, spice, pharmaceutical, chemical and petroleum industries [17]. The reaction mixtures, which were produced under the above optimal conditions, were purified by a rotary evaporator and two-step molecular distillation.…”

Section: Purification Of Camellia Oil-based Isopropyl Estermentioning

confidence: 99%

Enzymatic Synthesis of an Isopropyl Ester by Alcoholysis of Camellia Oil

Liu

Qin

Zheng-xiang

et al. 2012

A camellia oil-based isopropyl ester (CO-IPE) was successfully synthesized by enzymatic alcoholysis with camellia oil (CO), and its physiochemical properties were analyzed. Three commercial immobilized lipases (Lipozyme RM IM, Lipozyme TL IM and Novozym 435) were screened, and Novozym 435 was the best one. The optimal reaction conditions were achieved at 240 U/g of Novozym 435 loading, a substrate molar ratio of 5:1 (isopropanol/CO), and 24 h of reaction time at 55°C. Under the above conditions, the content of CO-IPE was obtained as 89.83%. Purity of CO-IPE further increased to be 96.95% after separation by rotary evaporation and molecular distillation. The viscosity of the synthesized CO-IPE showed itself to be about six times lower than that of CO, and the refractive index of the CO-IPE (1.449) was nearer to 1 in contrast to that of CO. It suggested that CO-IPE could be more intensively applied in the cosmetic industry.

“…Molecular distillation works at low pressures and high temperatures, and has advantages over other techniques that use solvents as separating agents [10]. It has been regarded as the most suitable distillation method for separating and purifying heat-sensitive products such as: fatty accompaniments [11,12]; monoacylglycerols and diacylglycerols [13,14]; eicosapentaenoic acid and docosahexaenoic acid ethyl esters [15,16]; as well as for removing undesirable molecules [17]. Furthermore, molecular distillation also traditionally plays a significant role in oil refining (especially in oils with high FFA contents such as RBO) in the deacidification and deodorization processes [10,18,19].…”

Section: Introductionmentioning

confidence: 99%

Production of Rice Bran Oil with Light Color and High Oryzanol Content by Multi‐stage Molecular Distillation

Jin

Xie

Chen³

et al. 2015

SRO-II and SRO-III had a clear yellow appearance and a non-triacylglycerol content <1.5 %. These semi-refined oils were mixed directly after MMD and further refined by mild deacidification and winterization to obtain fully refined oil, which met the requirements of commonly used standards. Notably, the final oil exhibited light color and retained nearly 80 % of the oryzanol of raw oil. The yield of final oil reached 80-85 % through the entire refining process.