Evaluation of total flavonoid content and analysis of related EST-SSR in Chinese peanut germplasm

Hou, Mingyu; Zhang, Yongjiang; Cui, Shunli; Yang, Xinlei; Liu, Lifeng

doi:10.1590/1984-70332017v17n3a34

Cited by 14 publications

(11 citation statements)

References 25 publications

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“…Recently, Yu et al (2019) reported the highest content of rutin and quercetin in dark gray tartary buckwheat seed followed by dark brown, graybrown and brown. Although it has previously been established that the flavonoid content in seeds is closely related to seed color and seed shape (Hou et al 2017), in the present study, we failed to detect any significant differences in the mean rutin and quercetin content of germplasm possessing ovate, triangular, or winged-form seeds. The germplasm with winged-form seeds showed the highest mean rutin content (22.77 mg/100 g DW), followed by germplasm with triangular seeds (19.77 mg/100 g DW).…”

Section: Agro-morphological Characterization For Rutin and Quercetin contrasting

confidence: 99%

“…Additionally, several studies conducted on other major crop plants have also revealed an association between the seed color and phenolic components (Zhang et al 2010;Shao et al 2011;Wang et al 2015). Although higher flavonoid content was reported in the seed coats than in the embryos of soybean and rice (Bordiga et al 2014;Min et al 2015), it has been emphasized that the type and quantity of flavonoids in seed coats and embryos may be related to plant genotype (Hou et al 2017). Furthermore, Shen et al (2009) andShao et al (2014) found that black-and red-colored rice grains contain higher flavonoid content than white-colored grains.…”

Section: Agro-morphological Characterization For Rutin and Quercetin mentioning

confidence: 99%

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Evaluation of Fagopyrum esculentum Moench germplasm based on agro-morphological traits and the rutin and quercetin content of seeds under spring cultivation

Rauf

Yoon

Lee

et al. 2020

Genet Resour Crop Evol

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Buckwheat is an important grain crop known for its nutritional value and content of bioactive compounds, particularly rutin. In this study, we characterized diverse Fagopyrum esculentum Moench germplasm for the diversity of agro-morphological characteristics and the rutin and quercetin content in seeds under the spring cultivation. Of the 251 germplasm accessions assessed, 193 had red stems, 182 had a pale brown seed coat color, and 238 had ovate seeds. The times taken to reach 50% flowering and 80% maturity ranged from 38-45 to 73-95 days, respectively. The 1000-seed weight (TSW) varied from 21 to 42 g. Overall, the content of rutin and quercetin ranged from 7.22-47.86 to 0-1.22 mg/100 g DW, respectively. The number of days to flowering and maturity showed negative correlations with TSW and rutin and quercetin content. However, we found that at 73-80 days after sowing, early-maturing germplasm had significantly higher mean rutin content than either intermediate-or late-maturing germplasm.The TSW weight showed a positive correlation with the content of rutin and quercetin. We identified promising accessions based early maturity (\ 80 days), higher seed weight (C 35 g) and higher content of rutin (C 35 mg/100 g DW) and quercetin (C 1 mg/100 g DW). These accessions will help to enhance grain yield and the rutin and quercetin content in existing buckwheat cultivars for spring cultivation.

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Section: Agro-morphological Characterization For Rutin and Quercetin contrasting

confidence: 99%

Section: Agro-morphological Characterization For Rutin and Quercetin mentioning

confidence: 99%

Evaluation of Fagopyrum esculentum Moench germplasm based on agro-morphological traits and the rutin and quercetin content of seeds under spring cultivation

Rauf

Yoon

Lee

et al. 2020

Genet Resour Crop Evol

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“…Hence, the development of an effective marker system to assess genetic diversity in avocado collections facilitates the maintenance of germplasm and cultivar improvement. Transcriptome sequencing and de novo assembly has proven to be an important tool for gene discovery in many organisms and an effective method for molecular marker development [23][24][25]35]. De novo assembly of avocado cv.…”

Section: Discussionmentioning

confidence: 99%

“…Of the many DNA markers that have been developed, SSRs, which consist of repeated nucleotide motifs of between one and six bases, are widely preferred in plant genetics and breeding because they are widely distributed and abundant in plant genomes, and they are genetically codominant, highly reproducible, multi-allelic, and well suited for high-throughput genotyping [22][23][24][25]. Transcriptome sequencing, which is based on next-generation sequencing technologies, is a high-throughput technique that facilitates the acquisition of a large number of unigene sequences for expressed sequence tag (EST)-derived marker development [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Sequencing of Different Avocado Ecotypes: de novo Transcriptome Assembly, Annotation, Identification and Validation of EST-SSR Markers

Tan

et al. 2019

Forests

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Avocado (Persea americana Mill.) could be considered as an important tropical and subtropical woody oil crop with high economic and nutritional value. Despite the importance of this species, genomic information is currently unavailable for avocado and closely related congeners. In this study, we generated more than 216 million clean reads from different avocado ecotypes using Illumina HiSeq high-throughput sequencing technology. The high-quality reads were assembled into 154,310 unigenes with an average length of 922 bp. A total of 55,558 simple sequence repeat (SSR) loci detected among the 43,270 SSR-containing unigene sequences were used to develop 74,580 expressed sequence tag (EST)-SSR markers. From these markers, a subset of 100 EST-SSR markers was randomly chosen to identify polymorphic EST-SSR markers in 28 avocado accessions. Sixteen EST-SSR markers with moderate to high polymorphism levels were detected, with polymorphism information contents ranging from 0.33 to 0.84 and averaging 0.63. These 16 polymorphic EST-SSRs could clearly and effectively distinguish the 28 avocado accessions. In summary, our study is the first presentation of transcriptome data of different avocado ecotypes and comprehensive study on the development and analysis of a set of EST-SSR markers in avocado. The application of next-generation sequencing techniques for SSR development is a potentially powerful tool for genetic studies.

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“…Of the many available DNA markers, simple sequence repeats (SSRs) are commonly used for investigating plant genetics and breeding because they are widely distributed and abundant in plant genomes. They are also genetically codominant, highly reproducible, multi-allelic, and perfectly suitable for high-throughput genotyping [21][22][23][24][25]. Expressed sequence tag (EST)-derived markers in the genomic coding regions have an advantage over genomic DNA-derived markers, and can be efficiently amplified to reveal conserved sequences among related species [26].…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Long-Read Sequencing of Avocado Generates Microsatellite Markers for Analyzing the Genetic Diversity in Avocado Germplasm

Zang

Tan

et al. 2019

Agronomy

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Avocado (Persea americana Mill.) is an important fruit crop commercially grown in tropical and subtropical regions. Despite the importance of avocado, there is relatively little available genomic information regarding this fruit species. In this study, we functionally annotated the full-length avocado transcriptome sequence based on single-molecule real-time sequencing technology, and predicted the coding sequences (CDSs), transcription factors (TFs), and long non-coding RNA (lncRNA) sequences. Moreover, 76,777 simple sequence repeat (SSR) loci detected among the 42,096 SSR-containing transcript sequences were used to develop 149,733 expressed sequence tag (EST)-SSR markers. A subset of 100 EST-SSR markers was randomly chosen for an analysis that detected 15 polymorphicEST-SSR markers, with an average polymorphism information content of 0.45. These 15markers were able to clearly and effectively characterize46 avocado accessions based on geographical origin. In summary, our study is the first to generate a full-length transcriptome sequence and develop and analyze a set of EST-SSR markers in avocado. The application of third-generation sequencing techniques for developing SSR markers is a potentially powerful tool for genetic studies.

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Evaluation of total flavonoid content and analysis of related EST-SSR in Chinese peanut germplasm

Cited by 14 publications

References 25 publications

Evaluation of Fagopyrum esculentum Moench germplasm based on agro-morphological traits and the rutin and quercetin content of seeds under spring cultivation

Evaluation of Fagopyrum esculentum Moench germplasm based on agro-morphological traits and the rutin and quercetin content of seeds under spring cultivation

Transcriptome Sequencing of Different Avocado Ecotypes: de novo Transcriptome Assembly, Annotation, Identification and Validation of EST-SSR Markers

Single-Molecule Long-Read Sequencing of Avocado Generates Microsatellite Markers for Analyzing the Genetic Diversity in Avocado Germplasm

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