Evaluation of total phenol and flavonoid content and antimicrobial and antibiofilm activities of Trollius chinensis Bunge extracts on Streptococcus mutans

Abstract

Dental caries is a chronic disease with multiple bacterial infections, Streptococcus mutans is the main cariogenic bacteria. Trollius chinensis Bunge is a common folk medicine in the Xinjiang area of China. In this study, we investigated the total flavonoid content and total phenol content in four types of T. chinensis Bunge extracts and the inhibitory effects of these extracts on S. mutans. Agar diffusion method was used to measure the inhibition zone diameters, and the minimum inhibitory concentration and minimum bactericidal concentration were determined by the twofold dilution method. Water extracts from T. chinensis Bunge and ethanol (30, 60, and 90%) extracts at different concentrations could significantly inhibit the growth of S. mutans. Among them, 30% ethanol extract exhibited the best antibacterial and antibiofilms effect. Biofilm research (crystal violet staining and CLSM) showed that 30% ethanol extract of T. chinensis Bunge plays an important role in inhibiting S. mutans growth and the number of biofilms. The results indicate that T. chinensis Bunge extract has good antibacterial and anti-biofilm activity on S. mutans. It has the potential to be developed for the treatment of caries in clinical application.

Enhanced Spontaneous Antibacterial Activity of δ-MnO2 by Alkali Metals Doping

“…Furthermore, bacterial biofilm formation is known to increase resistance against the antibiotic and plays a critical role in the pathogenesis of infections ( Banerjee et al, 2020 ). Then, we also examined the effect of Mg-doped MnO 2 nanoflowers on S. mutans biofilm formation by crystal violet staining ( Asgharpour et al, 2019 ; Chan et al, 2020 ; Jiang et al, 2020 ; Song et al, 2020 ), and the results showed the same trend as the MTT assay ( Figure 3B ). In the presence of Mg-doped MnO 2 , the inhibition percentage of biofilm formation was 4.1 ± 1.45% at 100 μg/ml, 21.02 ± 0.49% at 200 μg/ml, 39.86 ± 1.59% at 400 μg/ml, and 51.99 ± 1.18% at 800 μg/ml concentration, respectively, showing a dose-dependent enhanced antibacterial activity and reduced biofilm formation against S. mutans induced by Mg-doped MnO 2 nanoflowers.…”

Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography

“…Flavonoids are metabolite compounds that have antibacterial activity, especially against S. mutans and S. dysenteriae. Extracts from several herbs containing flavonoids are known to actively inhibit the growth of S. mutans (Jiang et al, 2020) since they can penetrate the film membrane of S. mutans (Veloz et al, 2019). Flavonoids could form complex compounds with the constituent compounds of the bacterial cell wall (extracellular protein).…”

Optimization of Flavonoid Extraction from Abelmoschus manihot Flowers Using Ultrasonic Techniques: Predictive Modeling through Response Surface Methodology and Deep Neural Network and Biological Activity Assessment

“…The MIC and MBC of AMFE against S. mutans were determined by the microdilution method [ 50 ]. First, a 50 mg/mL solution of AMFE was prepared, which was aseptically filtered and then diluted to a concentration of 50–1 mg/mL using sterile ultrapure water.…”