Evaluation of toxicological, anti-allergic and neuropharmacological activities of <em>Bridelia stipularis</em>: <em>In-vivo</em> and in-silico studies

Abstract

Bridelia stipularis locally known as 'Harinhara' native in hill tract area. The plant is traditionally used in cough, asthma and catarrh by the Chakma ethnic people of Bangladesh. In this study, we investigated the toxicological, anti-allergic, neuropharmacological potential of leaves extract and profiling of its bioactive polyphenols by HPLC for in-silico molecular docking. Phytochemical test confirmed the existence of phytochemicals including tannin, flavonoid, saponin, alkaloid, glycoside etc. In toxicity study, the extract was safe up to 3 g/kg oral dose in mice. The studied showed no organ specific toxicity at 500 mg/kg oral dose. Anti-allergic activity performed by using TDI-induced allergic mice model. The extract significantly reduced the TDI-induced allergy like symptoms such as scratching, sneezing, swelling (P < 0.05). Additionally, the extract also ameliorated the leukocytes count in blood and bronchoalveolar lavage (BAL) fluid. The neuropharmacological activity test was conducted by following the open field method in mice model, at both doses of extract at 300 and 500 mg/kg showed mild CNS depressant effect. HPLC profiling identified the presence of seven phenolic compounds, among these rutin hydrate demonstrated the best docking score of -7.4 kcal/mol same as anti-allergic drug against (H1R). On the other hand exhibited rosmarinic acid and myricetin -7.9 and -7.8 kcal/mol against GABAA whereas standard CNS drug showed -7.7 kcal/mol. In conclusion, B. stipularis is safe and rich in different polyphenolic compounds which were found effective against diseases.

Antiallergic activity of Amoora cucullata Roxb bark extract and profiling of its polyphenolic compounds: In-vivo and in-silico studies

“…People of rural areas are using these herbal drugs traditionally over the years due to the availability of the medicinal plants as well as high expenses and side effects of allopathic drugs. Several medicinal plants are popular as healing agents for allergic disorders without any scientific evaluation 15 . Since traditional medicine could be a reliable source of drug discovery and development, researchers are prone to explore these valuable natural resources in order to find out more improved therapy for allergic conditions.…”

“…Nasal allergy-like symptoms (sneezing, scratching, and nasal score) were measured following the method described previously 15 . In brief, just after TDI provocation, the number of sneezes and scratches were counted carefully in each mouse for 10 minutes.…”