Evaluation of transgenic peanut plants encoding coat protein and nucleocapsid protein genes for resistance to tobacco streak virus and peanut bud necrosis virus

Patil, Abhinandan; Thankappan, Ratheeshkumar; Mehta, Reetu; Yadav, Reena; Kumar, Abhay; Mishra, Gyan P.; Dobaria, Jentilal R.; Thirumalaisamy, P. P.; Jain, Ritu

doi:10.22438/jeb/38/2/ms-191

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…RT-qPCR is one of the most widely used methods for gene expression studies in mRNA levels, because of it's sensitivity, specificity, accuracy and cost-effectiveness (Gachon et al, 2004;Nolan et al, 2006;Patil et al, 2017). However, gene expression data obtained using RT-qPCR can be affected by different variables, such as quality and amount of starting material, RNA quality (purity, integrity, DNA contamination), reverse transcription efficiency and amplification efficiency etc.…”

Section: Introductionmentioning

confidence: 99%

Selection of reference genes for RT-qPCR analysis in the bark of Populus yunnanensis cuttings

Tian¹,

Li²,

Zu³

et al. 2019

JEB

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The aim of this paper was to select and evaluate the stability of seven candidate reference genes (PD-E1, TUA2, UBQ, ACT2, TUB, HIS and 18S RNA) under three different conditions for RT-qPCR analysis. Cuttings of Populus yunnanensis were cultured in water by two ways, upright and inverted vertically. Different internode barks were collected as test materials from 0 day to 49 day, once in seven day. Upright samples (U) and inverted samples (I) are two different conditions, total samples (U + I) means the third condition. Seven candidate reference genes (PD-E1, TUA2, UBQ, ACT2, TUB, HIS and 18S RNA) were amplified by RT-qPCR. geNorm, NormFinder, BestKeeper and RankAggreg programs were used to select and evaluate the stability of seven candidate reference genes under three different conditions.

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Section: Introductionmentioning

confidence: 99%