Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt

Dubey, S. C.; Suresh, M.; Singh, B. D.

doi:10.1016/j.biocontrol.2006.06.006

Cited by 277 publications

(185 citation statements)

References 11 publications

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“…Conforme verificado in vitro, é provável que as diferenças no percentual de incidência do patógeno e de plântulas normais oriundas das sementes infectadas tenha sido causada pela ação desses metabólitos, para todos os isolados de T. harzianum. De forma análoga, Dubey et al (2007), após selecionarem isolados de Trichoderma baseando-se em testes in vitro de cultura pareada e metabólitos voláteis e não voláteis, também verificaram o controle in vivo de F. oxysporum f.sp. ciceris ao realizar a microbiolização com 10 6 conídios de Trichoderma spp.…”

Section: Metabólitos Voláteis (%)unclassified

“…10 g -1 de sementes de ervilha, semeadas em vaso com solo infestado pelo patógeno. Embora tenham empregado menor concentração do antagonista, Dubey et al (2007) verificaram entre 21 e 30,6% de incidência de murcha nas plantas obtidas de sementes tratadas com T. harzianum contra 62 a 74% de incidência nas plantas obtidas de sementes sem tratamento. Tais valores foram muito próximos aos obtidos no presente trabalho, ao se avaliar a incidência nas sementes e plântulas infectadas por F. oxysporum f.sp.…”

Section: Metabólitos Voláteis (%)unclassified

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Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum

Carvalho¹,

Mello²,

Júnior³

et al. 2011

Trop. plant pathol.

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RESUMOEste trabalho objetivou avaliar seis isolados de Trichoderma harzianum no controle de Fusarium oxysporum f.sp. phaseoli em sementes, e seu efeito no crescimento inicial do feijoeiro comum. Os isolados foram inicialmente confrontados in vitro com o patógeno em testes de cultura pareada e antibiose a 25ºC. Sementes sadias e artificialmente infectadas pelo patógeno foram microbiolizadas com 2 mL de suspensões dos antagonistas (2,5 x 10 8 conídios mL -1 ) para cada 100 g de sementes, em rolo de papel germtest a 20 e 25°C. Os percentuais de incidência do patógeno e de plântulas normais foram avaliados aos sete e nove dias, respectivamente. Em casa de vegetação, os isolados foram aplicados a 5 x 10 9 conídios 500 g -1 de substrato autoclavado, com avaliação do comprimento das raízes e parte aérea das plantas 11 dias após o semeio (12 plantas tratamento -1 ). Todos os isolados apresentaram antagonismo in vitro contra o patógeno. Os isolados CEN202, CEN234, CEN238, CEN240 foram superiores à testemunha no controle de F. oxysporum em sementes, reduzindo entre 35 e 51% da incidência do patógeno e proporcionando entre 73 e 81% de plântulas normais. O comprimento total das plantas com tratamento CEN239 (37,43 cm) foi superior aos demais, cujo comprimento variou entre 27,84 e 33,95 cm. Palavras-chave: Phaseolus vulgaris, controle biológico, patologia de sementes, supressão de crescimento. ABSTRACT Control of Fusarium oxysporum f.sp phaseoli in vitro and on seeds and growth promotion of common bean in early stages by Trichoderma harzianumThe objective of this study was to evaluate the antagonistic capacity of six isolates of Trichoderma harzianum against Fusarium oxysporum f.sp. phaseoli on infected seeds and its effect on the early stages of growth in bean plants. The isolates were also evaluated in dual culture assays and under in vitro antibiosis at 25ºC. Healthy and infected seeds were treated with 2 mL 100 g -1 seeds of the antagonist suspensions (2.5 x 10 8 conidia mL -1 ) for healthy evaluation using blotter method at 20 and 25°C. The percentages of pathogen incidence and normal seedlings were evaluated at seven and nine days, respectively. The isolates were applied at 5 x 10 9 conidia 500 g -1 of sterilized substrate in greenhouse. After 11 days, roots and shoots of the bean plants were measured (12 plants treatment -1 ). Every isolate of T. harzianum presented in vitro antagonism against the pathogen. The strains CEN202, CEN234, CEN238 and CEN240 offered better control of F. oxysporum on seeds than the positive check, causing a reduction between 35 and 51% in pathogen incidence and 73-81% of normal seedling emergence. The total length of the plants treated with CEN239 (37.43 cm) was longer than the other treatments, with length varying between 27.84 and 33.95 cm.

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Section: Metabólitos Voláteis (%)unclassified

Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum

Carvalho¹,

Mello²,

Júnior³

et al. 2011

Trop. plant pathol.

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“…are widely used in agriculture as biocontrol agents because of their ability to reduce the incidence of disease caused by plant pathogenic fungi, particularly many common soil borne pathogens [21][22][23][24][25][26][27][28][29]. In present work, in vitro antagonism of 7 species of Trichoderma with M. phaseolina, causing charcoal rot in castor, were studied.…”

Section: Discussionmentioning

confidence: 99%

Antagonism of Trichoderma spp. against Macrophomina phaseolina: Evaluation of Coiling and Cell Wall Degrading Enzymatic Activities

HP¹

2012

J Plant Pathol Microb

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Materials and Methods Sources of Trichoderma sppSlants of six species of Trichoderma (T. harzianum NBAII Th 1; T. viride NBAII Tv 23; T. virens NBAII Tvs 12; T. koningi MTCC 796; T. pseudokoningi MTCC 2048; T. hamatum NBAII Tha-1.) were obtained either from IARI, New delhi or from MTCC, Chandigarh. One local isolate of T. harzianum was also collected from culture collection of Department of Plant Pathology, Junagadh Agricultural University, Junagadh. Isolation of phytopathogenic fungiMacrophomina phaseolina (Tassi) Goid was used as pathogen. The castor plants showing typical symptoms of root rot were collected AbstractIn vitro potentialities of seven species of Trichoderma were evaluated against phytopathogen Macrophomina phaseolina by dual culture techniques. The maximum growth inhibition of test pathogen was observed by antagonist T. koningi MTCC 796 (T4) (74.3%) followed by T. harzianum NABII Th 1 (T1) (61.4%) at 7 days after inoculation (DAI). Further, mycoparasitism of antagonists were observed upto 14 DAI. Pattern of growth inhibition of test fungus was continued with maximum 14.7% increases in T4 (85.2%) followed by 6.8% elevation in T1 (65.6%) antagonists during 7 to 14 DAI. Microscopic study showed that these two antagonists were capable of overgrowing and degrading M. phaseolina mycelia, coiling around the hyphae with apressoria and hook-like structures. At 14 DAI, T. koningi MTCC 796 completely destroyed the host and sporulated. The specific activities of cell wall degrading enzymes-chitinase, β-1, 3 glucanase, protease and cellulase were tested during different incubation period (24, 48, 72 and 96 h) when Trichoderma spp. grew in presence of pathogen cell wall in synthetic media. The antagonist T. koningi MTCC 796 induced higher chitinase and protease activity at 24 h incubation while β-1, 3 glucanase activities was elevated 1.18 fold during 72 to 96 h. Total phenol was produced significantly higher in culture supernatant of T. koningi MTCC 796 antagonist followed by T. hamatum NBAII Tha 1 and T. harzianum NBAII Th 1 at 48 h incubation. The growth inhibitions of pathogen during antagonism was positively correlated with coiling pattern of antagonists at 14 DAI, and induction of chitinase, β-1, 3 glucanase and total phenol content.

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“…Many researchers reported that soil amendment with formulated Trichoderma was effective in controlling seedling disease of many crops especially the fungal pathogens such as R. solani, Fusarium oxysporum and S. rolfsii (Lo et al, 1996;Tran, 1998;Bari et al, 2000;Shamsuzzaman et al, 2003;Benítez et al, 2004;Clear and Valic, 2005;Ngo et al, 2006;Shalini et al, 2006;Dubey et al, 2007;Rojo et al, 2007). The effect of Trichoderma spp.…”

Section: Efficacy Of Substrates Based T Harzianum Bio-fungicides Andmentioning

confidence: 99%

Effect of substrates to formulate Trichoderma harzianum based bio-fungicide in controlling seedling disease (Rhizoctonia solani) of brinjal

Faruk

Rahman

2017

Bangladesh J. Agric. Res

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Efficacy of three different substrates viz., rice bran, wheat bran, grass pea bran and their combinations with mustard oilcake (MOC) were tested to formulate a suitable Trichoderma harzianum based bio-fungicide for controlling seedling disease of brinjal caused by Rhizoctonia solani in tray soil as well as in seedbed soil under net house condition of Bangladesh Agricultural Research Institute (BARI), Gazipur during 2010 to 2014. The results of three years experiments revealed that T. harzianum bio-fungicides formulated in five different combinations of substrates viz., (1) rice bran + wheat bran, (2) rice bran + mustard oilcake (MOC) (3) rice bran + grasspea bran, (4) rice bran + wheat bran + MOC and (5) rice bran + grasspea bran +MOC were equally effective to control the soil borne seedling disease of brinjal caused by Rhizoctonia solani in tray soil and seedbed condition. In addition, vegetative growth of brinjal seedlings viz., shoot length, shoot weight, root length and root weight were enhanced significantly by the T. harzianum bio-fungicides in R. solani inoculated seedbed condition.

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Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt

Cited by 277 publications

References 11 publications

Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum

Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum

Antagonism of Trichoderma spp. against Macrophomina phaseolina: Evaluation of Coiling and Cell Wall Degrading Enzymatic Activities

Effect of substrates to formulate Trichoderma harzianum based bio-fungicide in controlling seedling disease (Rhizoctonia solani) of brinjal

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