Evaluation of two chemiluminescent assays compared with radioimmunoassay for serum progesterone measurement in bitches

Tal, Smadar; Mazaki‐Tovi, Michal; Druker, Shaked; Novak, Shiri; Raz, Tal; Aroch, Itamar

doi:10.1016/j.theriogenology.2019.11.026

Cited by 13 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, the clinical utility of LC-MS to measure canine serum P4 is limited (39,40,42,43). Historically, RIA has been regarded as the industry standard for canine serum P4 measurements, and correlates well to the more widely used CLIA (30)(31)(32)(33), the current industry standard used by most veterinary reference laboratories…”

Section: Discussionmentioning

confidence: 99%

“…Due to its inherent radiation hazard, equipment cost, decreased availability of reagents, and result reporting of 24 h or longer, very few reference laboratories currently offer serum P4 measurements via RIA in dogs (30,31). Consequently, chemiluminescent immunoassay (CLIA), which uses fluorescence and anti-P4 antibodies, has become the contemporary industry standard offered by reference laboratories due to its lower cost and faster turnaround time compared to RIA (30)(31)(32)(33). A comparative study of RIA and CLIA measurements of canine serum P4 concentration determined the two tests to be highly correlated (r = 0.9709; P < 0.0001) (9).…”

Section: Introductionmentioning

confidence: 99%

“…Radioimmunoassay (RIA) is the historical industry-standard for canine serum P4 measurement but has limited availability. Due to its inherent radiation hazard, equipment cost, decreased availability of reagents, and result reporting of 24 h or longer, very few reference laboratories currently offer serum P4 measurements via RIA in dogs ( 30 , 31 ). Consequently, chemiluminescent immunoassay (CLIA), which uses fluorescence and anti-P4 antibodies, has become the contemporary industry standard offered by reference laboratories due to its lower cost and faster turnaround time compared to RIA ( 30 – 33 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of a Point-of-Care Analyzer With a Chemiluminescent Immunoassay for Serum Progesterone Measurement in Breeding Management of the Bitch

et al. 2021

View full text Add to dashboard Cite

Accurate serum progesterone measurements for timing bitches during breeding management is critical for reproductive practice, especially as artificial insemination has become routine to facilitate breeding of animals that are geographically or temporally separated. To measure serum progesterone, chemiluminescent immunoassay (CLIA) has replaced radioimmunoassay as the current standard in the bitch due to its high correlation and increased practicality. In January 2019, a colorimetric point-of-care (POC) immunoassay for quantitative in-clinic canine serum progesterone measurements in <30 min was released. This study provides an independent comparison of the POC (Catalyst One, IDEXX) to the current industry standard, CLIA (Immulite-2000, Siemens). To assess inter-assay imprecision of POC and agreement of the POC and CLIA results, 100 canine serum samples were analyzed on three analyzers (POC-1, POC-2, and CLIA), of which, 74 (POC-1) and 75 (POC-2) results were within POCs' reportable range of 0.2–20 ng/mL and included in the study. To assess intra-assay imprecision, pooled canine serum samples at low (L1), intermediate (L2), and high (L3) progesterone concentrations were analyzed ten times each on POC-1 and CLIA. Relative to CLIA, POC values showed good correlation (POC-1, r2 = 0.9366; POC-2, r2 = 0.9438, P < 0.0001) and significant positive proportional bias at values >2 ng/mL. The POC inter-assay coefficients of variation (CVs) were 13.2% (0.2–2.9 ng/mL, 0.6–9.2 nmol/L, L1), 10.0% (3.0–9.9 ng/mL, 9.5–31.5 nmol/L, L2), 7.1% (10.0–20.0 ng/mL, 31.8–63.6 nmol/L, L3), and 11.2% (all samples). The intra-assay CVs for POC (L1, 15.3%; L2, 7.0%; L3, 4.7%) were higher than those for CLIA (L1, 5.89%; L2, 4.89%; L3, 3.44%). Based on the more rapid increase in serial serum progesterone concentrations in ovulating bitches and the greater imprecision of the POC, the clinical interpretations of serum progesterone measurements as they relate to canine breeding management should be made with caution.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of a Point-of-Care Analyzer With a Chemiluminescent Immunoassay for Serum Progesterone Measurement in Breeding Management of the Bitch

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The purpose of the present study was to assess the reliability of this assay by comparing P4 concentrations measured with the SPFS assay with those measured with CLIA, the perceived gold standard. Other authors preferred using different reference standards, such as RIA 13,14,22 or liquid chromatography-tandem mass spectrometry. 23 However, RIA requires specialized laboratory equipment and radioactive molecules, such that performing the assay may be hazardous to laboratory personnel and the environment.…”

Section: Discussionmentioning

confidence: 99%

“…However, results measured with different CLIA instruments b,c may be possible and, therefore, affect comparisons with RIA. 22 Liquid chromatography-tandem mass spectrometry, which is also considered as a reference standard for measurement of P4 concentration, was not chosen as a reference standard in the present study because of its unavailability and its limited use in veterinary medicine. 23 Results of the present study with a large number of blood samples indicated that SPFS was a reliable assay for determining P4 concentrations over the range of concentrations for the enrolled bitches.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of surface plasmon field-enhanced fluorescence spectroscopy for rapid measurement of progesterone concentration in bitches

Fontbonne

Maenhoudt

Thoumire

et al. 2021

ajvr

View full text Add to dashboard Cite

OBJECTIVE To compare progesterone (P4) concentrations measured with surface plasmon field-enhanced fluorescence spectroscopy (SPFS) and chemiluminescence immunoassay (CLIA) in serum and plasma samples of client-owned bitches of various ages and breeds and to determine reference ranges for P4 concentrations at various stages of the estrous cycle. SAMPLES 102 serum samples and 104 plasma samples. PROCEDURES In experiment 1, 1 aliquot each of serum and plasma was analyzed for P4 concentration by use of SPFS incorporated in a veterinary-specific point-of-care immunologic analyzer and CLIA. In experiment 2, serum collected from bitches in various stages of the estrous cycle was analyzed for P4 concentration by use of SPFS to establish reference ranges for each stage. RESULTS In experiment 1, P4 concentrations measured by SPFS and CLIA were highly correlated (serum, r = 0.966; plasma, r = 0.968). In experiment 2, ranges of serum basal (proestrous) P4 concentrations (n = 114) and P4 concentrations at the estimated time of ovulation (76), during pregnancy or diestrus (107), and during the prepartum period (50) measured with SPFS were 0.42 to 1.46 ng/mL, 3.69 to 7.85 ng/mL, 11.73 to 28.24 ng/mL, and 1.54 to 3.22 ng/mL, respectively. CONCLUSIONS AND CLINICAL RELEVANCE Because serum and plasma P4 concentrations measured with SPFS were highly correlated with those measured with CLIA and ranges of serum P4 concentrations measured with SPFS for each of phase of the estrous cycle were well-defined for the large sample size, veterinarians may be able to accurately use this veterinary-specific point-of-care immunologic analyzer with SPFS methodology to determine P4 concentrations of bitches in their daily practice.

show abstract

References

2023

Radioactivity

View full text Add to dashboard Cite

Evaluation of two chemiluminescent assays compared with radioimmunoassay for serum progesterone measurement in bitches

Cited by 13 publications

References 20 publications

Comparison of a Point-of-Care Analyzer With a Chemiluminescent Immunoassay for Serum Progesterone Measurement in Breeding Management of the Bitch

Comparison of a Point-of-Care Analyzer With a Chemiluminescent Immunoassay for Serum Progesterone Measurement in Breeding Management of the Bitch

Evaluation of surface plasmon field-enhanced fluorescence spectroscopy for rapid measurement of progesterone concentration in bitches

References

Contact Info

Product

Resources

About