Evaluation of two doses of recombinant luteinizing hormone supplementation in an unselected group of women undergoing follicular stimulation for in vitro fertilization

Lisi, Franco; Rinaldi, Leonardo; Fishel, Simon; Caserta, Donatella; Lisi, Rosella; Campbell, Alison

doi:10.1016/j.fertnstert.2004.07.969

Cited by 58 publications

(38 citation statements)

References 39 publications

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“…The study population consisted of ICSI patients with major indications for ART such as tubal infertility or unexplained infertility and/ or partners with semen abnormalities. Average patient age was 33 years (range [31][32][33][34][35][36][37][38][39][40][41][42]. All patients were with regular menstrual cycles of 25-32 days, presumed to be ovulatory.…”

Section: Patientsmentioning

confidence: 99%

“…Down-regulation with GnRH-a in some normogonadotrophic women may result in a profound suppression of endogenous LH concentration impairing adequate E 2 synthesis. The stimulation with recombinant FSH (r-FSH) alone, containing negligible quantity of LH, has been shown to interfere with optimal oocyte maturation [17,56,58], while providing LH activity in gonadotropin treatment after GnRH-a down-regulation, has been shown to induce a significant higher proportion of top-quality embryos [37].…”

Section: Introductionmentioning

confidence: 99%

“…However, in women above 35 years of age, poor responders, or with more severe pituitary GnRH-a suppression, stimulation with r-LH in the mid/late-follicular phase could be associated with improved results [6,12,24,34,37,40,41,47]. In a recent meta-analysis study it has been suggested that the supplementation with r-LH in patients of advanced reproductive age stimulated with r-FSH, may improve implantation and clinical pregnancy without affecting metaphase II (Met II) yield [31].…”

Section: Introductionmentioning

confidence: 99%

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Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Barberi

Ermini

Morelli

et al. 2012

J Assist Reprod Genet

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Purpose Down-regulation with gonadodropin-releasing agonist (GnRH-a) protocol during IVF stimulation leads to a severe endogenous LH suppression, which may affect the follicular development. The aim of the study was to evaluate the effects of recombinant LH (r-LH) administration, during late follicular development stages, in recombinant FSH (r-FSH) stimulated cycles on follicular fluid (FF) parameters and on cumulus cell quality. Methods Twenty patients undergoing IVF were stimulated in a long GnRH agonist protocol with r-FSH alone or with r-LH supplementation when the leading follicle reached diameter of 14 mm. FF was collected at the time of oocyte retrieval from 32 follicles ≥ 18 mm. Serum FSH, LH, estradiol (E 2 ), and progesterone (P 4 ) were evaluated on the day of hCG administration. Intra-follicular E 2 , P 4 , AMH and TGF-β were assayed. Total RNA from 18 individual cumuli was isolated for gene expression analyses. Results R-LH increased FF P 4 levels. FF TGF-β levels and PTGS2 and HAS2 expression in cumulus cells (CCs) positively correlated with increased P 4 levels observed in FFs, while a negative correlation was found between P 4 and AMH levels. Conclusions FF positive correlation between P 4 and TGF-β levels and CC expression of PTGS2 and HAS2 suggest an association with a better follicle quality. In addition, our data suggest that late follicular phase r-LH supplementation leads to a more advanced stage of follicular maturation.

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Section: Patientsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Barberi

Ermini

Morelli

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…While direct measures of oocyte quality are difficult to assess clinically, some studies have noted an increased fertilization rate in oocytes obtained from cycles stimulated with LH (24,30). Numerous trials have also demonstrated that the addition of LH activity results in an increase in serum estradiol on the day of hCG (Figure 2), which may represent a higher quality cohort of developing follicles (22,26,30,(35)(36)(37)(38)(39)(40)(41)(42)(43). LH supplementation was demonstrated to result in lower levels of apoptosis in cumulous cells as compared to FSH stimulation only (44).…”

Section: Potential Mechanisms Of Exogenous Lh Benefit In Artmentioning

confidence: 99%

“…There are numerous RCT evaluating rLH plus rFSH versus rFSH alone, but the data is complicated by significant heterogeneity between the trials (29,30,(36)(37)(38)(39)(40)(41)(86)(87)(88)(89)(90)(91)(92)(93)(94)(95). The fact that the rLH dose can be administered at a separate starting time and doses from the rFSH dose has allowed researchers and clinicians to be more varied in the approach to rLH administration as compared to hMG.…”

Section: Recombinant Luteinizing Hormonementioning

confidence: 99%

The Use of rLH, HMG and hCG in Controlled Ovarian Stimulation for Assisted Reproductive Technologies

Hill¹,

Propst²

2012

Enhancing Success of Assisted Reproduction

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Recombinant Luteinizing Hormone (rLH) for controlled ovarian hyperstimulation in assisted reproductive cycles

Mochtar

Veen

Ziech

et al. 2007

Cochrane Database of Systematic Reviews

101

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Analysis 1.4. Comparison 1 rLH and rFSH versus rFSH alone for COH in GnRH agonist downregulated IVF or ICSI treatment cycles, Outcome 4 Serum oestrodial (pg/mL) on hCG day. . . . . . . . . . . . . . . . Analysis 1.5. Comparison 1 rLH and rFSH versus rFSH alone for COH in GnRH agonist downregulated IVF or ICSI treatment cycles, Outcome 5 Mean number of retrieved oocytes per oocyte retrieval. . . . . . . . . . Analysis 1.6. Comparison 1 rLH and rFSH versus rFSH alone for COH in GnRH agonist downregulated IVF or ICSI treatment cycles, Outcome 6 Clinical pregnancy per woman randomised. . . . . . . . . . . . . . Analysis 1.7. Comparison 1 rLH and rFSH versus rFSH alone for COH in GnRH agonist downregulated IVF or ICSI treatment cycles, Outcome 7 Ongoing pregnancy per woman randomised. . . . . . . . . . . . . . Analysis 1.8. Comparison 1 rLH and rFSH versus rFSH alone for COH in GnRH agonist downregulated IVF or ICSI treatment cycles, Outcome 8 Miscarriage rate per woman randomised. . . . . . . . . . . . . . . Analysis 2.1. Comparison 2 rLH and rFSH versus rFSH alone for COH in GnRH antagonist downregulated IVF or ICSI treatment cycles, Outcome

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Evaluation of two doses of recombinant luteinizing hormone supplementation in an unselected group of women undergoing follicular stimulation for in vitro fertilization

Cited by 58 publications

References 39 publications

Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

The Use of rLH, HMG and hCG in Controlled Ovarian Stimulation for Assisted Reproductive Technologies

Recombinant Luteinizing Hormone (rLH) for controlled ovarian hyperstimulation in assisted reproductive cycles

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