Evaluation of two ELISA kits for the detection of Aujeszky's disease antibodies in pigs

Abstract

This work describes the evaluation of two commercial ELISA kits for the detection of gI antibodies against Aujeszky's disease. A collection of experimental sera from infected pigs, field sample sera, and sera from pigs vaccinated with seven different modified gI-negative commercial vaccines were used to evaluate each test. Both ELISA kits showed good reproducibility, and specificity, but differences could be appreciated in sensitivity when sera obtained at early stages of infection was analysed. These results also indicated that both kits could be used in conjunction with the seven vaccines evaluated in this study.

Herd factors associated with the seroprevalences of four major respiratory pathogens in slaughter pigs from farrow-to-finish pig herds

“…The median within-herd seroprevalences were 76% for Mh, 100% for H 1 N 1 , 40% for H 3 skewed with a larger tail towards the lower seroprevalences, whereas the within-herd seroprevalences of the influenza H 1 N 1 virus showed less variation because almost all FP were seropositive. The distribution of the within-herd seroprevalences of ADV, and to a lesser extent of the influenza H 3 N 2 virus, showed a bimodal distribution with only 13% and 21% of the herds in the range of 20 to 80% seroprevalence, respectively.…”

“…Only sera of herds where FP were not vaccinated against influenza were analysed (n = 115). The presence of anti-gE-antibodies against ADV wild virus was determined with the Ingelvac ® Aujeszky ELISA (Svanova Diagnostics, Uppsala, Sweden) [3]. This test allows differentiation of a vaccinated pig from an infected pig.…”

“…The 4 serological tests have a high specificity and sensitivity [3,33,35]. None of these tests discriminated between different isotype classes of immunoglobulins.…”