Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus

Swierkosz, Ella M.; Arens, Max Q.; Schmidt, Rebecca; Armstrong, Thomas

doi:10.1128/jcm.21.4.643-644.1985

Cited by 17 publications

(7 citation statements)

References 4 publications

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“…Consequently, it would be unrealistic to suggest that monoclonal antibodies prepared against a single epitope would react with all isolates. The specificity of monoclonal antibodies and the antigenic complexity of HSV strains may therefore explain, in part, discordant typing results reported in this and other studies (4,9). It is interesting that for the 108 positive specimens tested, ENI type-common and HSV-2 typespecific monoclonal antibodies displayed a sensitivity and specificity of 100%.…”

Section: Resultssupporting

confidence: 43%

“…Swierkosz and co-workers (9) recently reported that background fluorescence probably obscured the determination of HSV (by the Virgo system) among 4 of 10 HSV-1 confirmed isolates. Their results are difficult to explain, as background fluorescence was not a problem in this study.…”

Section: Resultsmentioning

confidence: 99%

“…This procedure is relatively rapid, may be used in conjunction with isolation, and has the potential for unambiguous differentiation of HSV-1 from HSV-2. Recently, the sensitivities of several commercially available monoclonal antibodies for the culture confirmation and typing of HSV isolates have been questioned (4,9), thereby suggesting differences in the quality of products between laboratories.…”

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confidence: 99%

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Evaluation of three immunofluorescence assays for culture confirmation and typing of herpes simplex virus

Lipson¹,

Schutzbank²,

Szabo³

1987

J Clin Microbiol

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(KL), were evaluated for the laboratory confirmation and typing of herpes simplex virus (HSV). Of 108 coded HSV slide preparations, run in parallel with each monoclonal-antibody set, 103 were equivalent by the immunofluorescence assays. Among the five discordant isolates, three (2.8%) did not type with the KL monoclonal antibodies and two (1.9%) false-positive results occurred with the Syva typing system. All of the HSV clinical isolates tested were correctly typed with the ENI indirect immunofluorescence antigen detection system. Typing confirmation of the five discordant HSV isolates was performed by differential sensitivity to 5-bromo-2'-deoxyuridine and endonuclease cleavage analysis of the viral DNA. Use of the Syva and KL direct immunofluorescence antigen detection systems for the identification of HSV isolates is less time-consuming than use of the ENI indirect antigen detection system; however, sensitivity and specificity may be lost. * Corresponding author. medium (Hanks balanced salt solution) containing 0.5% gelatin, 50 ,ug of gentamicin per ml, and 100 U of mycostatin per ml. Specimens not immediately sent to the Virology Laboratory were temporarily stored (i.e., 24 to 38 h) at 4°C. Specimens were received from Nassau County Medical Center clinics, more than 13 hospitals, and approximately 30 physicians in group or individual practice throughout Nassau, Suffolk, Queens, and Manhattan Counties, N.Y. Virus isolation. Ail specimens received by the Virology

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Section: Resultssupporting

confidence: 43%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of three immunofluorescence assays for culture confirmation and typing of herpes simplex virus

Lipson¹,

Schutzbank²,

Szabo³

1987

J Clin Microbiol

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“…Our experience underscores the problem with relying exclusively on MAb-based detection systems in a diagnostic virology laboratory. Viral variants that occasionally fail to react with monoclonal reagents do occur (12).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a naturally occurring parainfluenza 3 virus variant

et al. 1995

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A parainfluenza 3 virus variant which failed to react with parainfluenza 3 virus-specific monoclonal antibodies from two commercial sources was isolated from a 14-month-old boy. Analysis of the coding region of the hemagglutinin-neuraminidase gene identified 36 nucleotide changes and 4 amino acid changes compared with a consensus sequence derived from strains isolated from 1957 through 1983. Two unique amino acid changes occurred at positions 174 and 283, which are close to identified epitopes in the hemagglutinin-neuraminidase protein. Ongoing viral surveillance to detect variants is important, particularly in regard to vaccine development.

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“…The clinical virology reagent market continues to have an influx of monoclonal antibody stains from different manufacturers who are capitalizing on the growing number of clinical virology laboratories (1,4,5,7,(10)(11)(12). With an increasing number of small community hospitals and laboratories delving into the area of HSV isolation, the demand for highquality low-cost HSV reagents has grown.…”

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confidence: 99%

Comparative evaluation of four commercially available monoclonal antibodies for culture confirmation of herpes simplex virus infection

Johnston

Wellens

1992

J Clin Microbiol

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Four fluorescein isothiocyanate-labelled monoclonal antibody typing reagents were compared for intensity of fluorescence and sensitivity in confirming herpes simplex virus (HSV) in cell culture. A total of 125 (50 HSV type 1 [HSV-1] and 75 HSV-2) specimens positive for HSV were concurrently stained with type-specific monoclonal antibodies from Bartels (Bartels Immunodiagnostic Supplies, Inc., Bellevue, Wash.), Kallestad (Pathfinder; Kallestad Diagnostics, Chaska, Minn.), Diagnostic Products Corp. (DPC) (PathoDx; DPC, Los Angeles, Calif.), and Syva (Microtrak; Syva Co., Palo Alto, Calif.). Fifty cultured specimens not displaying HSV-associated cytopathic effect were also stained. Each of the four reagents confirmed the 50 negative cultures and 125 positive cultures. One HSV-1-positive culture was missed by the Kallestad antibody. Ten HSV-1 isolates displayed dull fluorescence when stained with the Syva HSV-2 reagent. The other three reagents stained only the HSV-1 well with these 10 specimens. The Syva and Bartels stains were brighter, in general, than the Kallestad and DPC stains. However, the Kallestad and DPC stains were brilliant enough to allow interpretation without hesitation. The differences in intensity and background staining emphasize the need for laboratories to routinely perform quality checks on their reagents and to test new products which become commercially available.

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Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus

Cited by 17 publications

References 4 publications

Evaluation of three immunofluorescence assays for culture confirmation and typing of herpes simplex virus

Evaluation of three immunofluorescence assays for culture confirmation and typing of herpes simplex virus

Isolation and characterization of a naturally occurring parainfluenza 3 virus variant

Comparative evaluation of four commercially available monoclonal antibodies for culture confirmation of herpes simplex virus infection

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