Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella Enteritidis and Listeria monocytogenes in artificially contaminated ready-to eat fresh products

Birmpa, Angeliki; Kalogeropoulos, Konstantinos; Kokkinos, Petros; Vantarakis, Apostolos

doi:10.4081/ijfs.2015.5383

Cited by 9 publications

(7 citation statements)

References 45 publications

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“…Cherry tomato is a major garden product popular among consumers that is low in calories and rich in lycopene, vitamin C, and β‐carotene (Soto‐Zamora and others ; Rosales and others ; Yun and others ). It is usually sold ready to eat, and often consumed raw or in salads (Birmpa and others ). However, being a climacteric fruit, cherry tomato ripens, and senesces rapidly after harvest owing to transpiration and respiration, and is easily damaged and contaminated by microorganisms (Fagundes and others ).…”

Section: Introductionmentioning

confidence: 99%

Edible Coating Using a Chitosan‐Based Colloid Incorporating Grapefruit Seed Extract for Cherry Tomato Safety and Preservation

Won

Lee

Park

et al. 2017

Journal of Food Science

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Grapefruit seed extract (GSE)-containing chitosan-based coating was developed and applied to cherry tomatoes to protect them from Salmonella invasion and improve their storability. The coating colloids were produced by mixing a chitosan colloid (1% [w/w] chitosan) with GSE at various concentrations (0.5%, 0.7%, 1.0%, and 1.2% [w/w]) using high-shear mixing (10000 rpm, 2 min). Coatings with chitosan colloids containing GSE at 0.0%, 0.5%, 0.7%, and 1.0% (w/w) inactivated Salmonella on cherry tomatoes by 1.0 ± 0.3, 1.2 ± 0.3, 1.6 ± 0.1, and 2.0 ± 0.3 log CFU/cherry tomato, respectively. Coatings both with and without GSE (1.0%) effectively inhibited the growth of Salmonella and total mesophilic aerobes, reduced CO 2 generation, and retarded titratable acidity decrease during storage at 10 and 25°C. The advantage of incorporating GSE in the formulation was demonstrated by delayed microorganism growth and reduced weight loss at 25°C. The chitosan-GSE coating did not affect lycopene concentration, color, and sensory properties (P > 0.05). Chitosan-GSE coating shows potential for improving the microbiological safety and storability of cherry tomatoes, with stronger efficacy at 25°C than that of chitosan coating without GSE.Keywords: antimicrobial packaging, chitosan, edible coating, grapefruit seed, tomato Practical Application: A novel chitosan coating containing grape fruit seed extract (GSE) improved the microbiological safety against Salmonella and storability of cherry tomatoes without altering their flavor, demonstrating its strong potential as an effective postharvest technology. Chitosan coating containing GSE might be preferable over chitosan coating without GSE for application to tomatoes that are stored at room temperature in that it more effectively inhibits microbial growth and weight loss than the coating without GSE at 25°C.

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Section: Introductionmentioning

confidence: 99%

Edible Coating Using a Chitosan‐Based Colloid Incorporating Grapefruit Seed Extract for Cherry Tomato Safety and Preservation

Won

Lee

Park

et al. 2017

Journal of Food Science

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“…There were 3 (for IS6110) and 1 (for IS1081) RPA‐S reactions that exhibited an indefinite orange‐green color after adding SYBR Green I, which were interpreted as negative for Mtb detection. A previous study showed that there was no significant difference between the LAMP results detected by agarose gel electrophoresis or naked eye detection of LAMP products after SYBR Green I addition . Although SYBR Green I exhibits good sensitivity for DNA detection, false negatives somehow occur when the DNA content is low .…”

Section: Discussionmentioning

confidence: 98%

Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification—SYBR green I assays

Singpanomchai

Akeda

Tomono

et al. 2018

Clinical Laboratory Analysis

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“…It is a novel isothermal amplification method and is used in rapid diagnostic testing of viruses [1], bacteria [2], fungi [3], and parasites [4] in all sorts of biological samples. Derived from the famous classical polymerase chain reaction (PCR), it presents many advantages like to be low-cost, no thermocycler required, tolerant to sample matrix inhibitors And it performs with the various products detection methods like fluorescent dye, real-time detection, lateral flow assays, and gel analysis [ Fig.…”

Section: Introductionmentioning

confidence: 99%

Loop-mediated Isothermal Amplification LAMP, Simple Alternative Technique of Molecular Diagnosis Process in Medicals Analysis: A Review

Uwiringiyeyezu

Khalfi

Belhachmi

et al. 2019

ARRB

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The Medical diagnostic process requires more various methods to cover all sorts of clinical demands. Those methods depend on the types of samples and the requested analysis. The Medical diagnostic is considered as a process until making a clinical decision about a physiopathology or infection through medicals analysis. Nucleic acid amplification methods continue to play a role in these medicals analysis processes, but, many gold conventional standard Methods are time-consumers, less sensitive, technically challenging. It is that reason why recently, after the development of the PCR technique; the real-time PCR became a frequently requested tool to diagnose the virus, bacteria, fungal and parasites infections in different pathologies, especially in developed countries. However, its availability remains a big problem in resource-limited countries like Africa continent. It is because of the issue due to cost, technology, human resource constraints and the remote areas with limited access to laboratory facilities which stays also a major problem. Nucleic acids amplification methods remain to be expensive, not accessible for all laboratories. In addition to this, they are easily inhibited and contaminated by the other biochemical reagents time-consumer, for this reason, other methods of nucleic acid amplification under isothermal conditions have emerged each with its particularities. Despite their successes, molecular biology should have a method that combines many of the advantages and accessible everywhere. So, in 2000, the LAMP method was developed and offered many advantages such as speed, specificity, high sensitivity and the cost of very low equipment. In this review, we present the different studies to confirm the advantages of LAMP that we consider as an alternative to PCR in molecular diagnostics with a wide range of use on clinical samples and in scientific research and also, the method to save the Africans patients from the high cost and time consuming of other amplification methods.

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Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella Enteritidis and Listeria monocytogenes in artificially contaminated ready-to eat fresh products

Cited by 9 publications

References 45 publications

Edible Coating Using a Chitosan‐Based Colloid Incorporating Grapefruit Seed Extract for Cherry Tomato Safety and Preservation

Edible Coating Using a Chitosan‐Based Colloid Incorporating Grapefruit Seed Extract for Cherry Tomato Safety and Preservation

Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification—SYBR green I assays

Loop-mediated Isothermal Amplification LAMP, Simple Alternative Technique of Molecular Diagnosis Process in Medicals Analysis: A Review

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