Evaluation of type-B RR dimerization in poplar: A mechanism to preserve signaling specificity?

Djeghdir, Inès; Chefdor, Françoise; Bertheau, Lucie; Κουδούνας, Κωνσταντίνος; Carqueijeiro, Inês; Cruz, Pamela Lemos; Courdavault, Vincent; Depierreux, Christiane; Larcher, Mélanie; Lamblin, Frédéric; Héricourt, François; Glévarec, Gaëlle; Oudin, Audrey; Carpin, Sabine

doi:10.1016/j.plantsci.2021.111068

Cited by 4 publications

(2 citation statements)

References 63 publications

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“…Similar results can be found in the model plant of Arabidopsis. For CiRR18, its Arabidopsis counterpart ARR18 has been shown to be a positive regulator of drought stress and cell proliferation [56,57]. ARR5, one of the most highly cytokinin-induced genes [58], responds to various stresses including cold, dehydration, and salinity [59].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of the Response Regulator (RR) Gene Family in Pecan Reveals Its Possible Association with Callus Formation during Grafting

Zhang,

Jia,

Wang

et al. 2024

Forests

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Response regulator (RR) is the core component of cytokinin (CK) signaling, and it regulates the expression of numerous downstream CK-responsive genes. However, the knowledge regarding the pecan RR (CiRR) gene family is still limited. In this study, we first monitored trans-zeatin riboside (tZR) content in the graft union 0, 7, 14, and 32 days after grafting and then conducted genome-wide analysis and expression profiling of the CiRR gene family using an available genome sequence and RNA-seq dataset, aiming to better understand the roles of CK during pecan grafting. The dynamic contents of tZR showed an increased trend during the specific period for both the scion and rootstock. There were 20 CiRRs in the pecan genome, including 12 type A CiRRs, 5 type B members, and 3 type C genes. All members contained a receiver domain and type B CiRRs possessed an additional Myb-like DNA-binding domain. Promoter analysis showed that the CiRR gene family contained cis-elements associated with growth and development, hormones, and stress. A total of 10 genes, including CiRR18/9/4a/14a/12c/5/12b/14b/2b/2a, were abundantly expressed in the samples of different tissues, drought stress, and kernel development. There were 12 genes (CiRR5/18/4a/12b/2b/12c/14b/2a/14a/4b/9/11a) showing active expressions during grafting, and weighted gene co-expression network analysis (WGCNA) grouped them into six modules. Among them, CiRR14a and CiRR12b were the hub genes for the turquoise and brown modules, respectively. Functional annotation indicated that the turquoise module was associated with gene transcription and translation, while the brown module was related to cell proliferation. Our results suggest that the CiRR gene family central to CK signaling is probably involved in callus formation during pecan grafting.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of the Response Regulator (RR) Gene Family in Pecan Reveals Its Possible Association with Callus Formation during Grafting

Zhang,

Jia,

Wang

et al. 2024

Forests

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“…In Populus , two proteins homologous to AHK1 have been identified, namely HK1a and HK1b [ 13 , 14 ]. Unlike Arabidopsis, the full MSP for both poplar HK1s has been determined with HPt and RR partners [ 15 , 16 , 17 , 18 , 19 , 20 ]. These HK1s’ partners have been shown to be shared with the cytokinin (CK) MSP, showing interconnection between these two signalling pathways [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Searching for Osmosensing Determinants in Poplar Histidine-Aspartate Kinases

Makhokh

Lafite

Larcher

et al. 2023

IJMS

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Previous works have shown the existence of protein partnership, belonging to a MultiStep Phosphorelay (MSP), potentially involved in osmosensing in Populus. The first actor of this signalling pathway belongs to the histidine-aspartate kinase (HK) family, which also includes the yeast osmosensor Sln1, as well as the Arabidopsis putative osmosensor AHK1. In poplar, the homologous AHK1 protein corresponds to a pair of paralogous proteins, HK1a and HK1b, exhibiting an extracellular domain (ECD), as in Sln1 and AHK1. An ECD alignment of AHK1-like proteins, from different plant species, showed a particularly well conserved ECD and revealed the presence of a cache domain. This level of conservation suggested a functional role of this domain in osmosensing. Thus, we tested this possibility by modelling assisted mutational analysis of the cache domain of the Populus HK1 proteins. The mutants were assessed for their ability to respond to different osmotic stress and the results point to an involvement of this domain in HK1 functionality. Furthermore, since HK1b was shown to respond better to stress than HK1a, these two receptors constituted a good system to search for osmosensing determinants responsible for this difference in efficiency. With domain swapping experiments, we finally demonstrated that the cache domain, as well as the second transmembrane domain, are involved in the osmosensing efficiency of these receptors.

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Genome-wide characterization of RR gene family members in Zanthoxylum armatum and the subsequent functional characterization of the C-type RR

Hui,

Wu,

Zheng

et al. 2024

Plant Physiology and Biochemistry

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Evaluation of type-B RR dimerization in poplar: A mechanism to preserve signaling specificity?

Cited by 4 publications

References 63 publications

Genome-Wide Identification and Expression Profiling of the Response Regulator (RR) Gene Family in Pecan Reveals Its Possible Association with Callus Formation during Grafting

Genome-Wide Identification and Expression Profiling of the Response Regulator (RR) Gene Family in Pecan Reveals Its Possible Association with Callus Formation during Grafting

Searching for Osmosensing Determinants in Poplar Histidine-Aspartate Kinases

Genome-wide characterization of RR gene family members in Zanthoxylum armatum and the subsequent functional characterization of the C-type RR

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