Evaluation of UV-C Mutagenized Scheffersomyces stipitis Strains for Ethanol Production

Geiger, Melanie R.; Gibbons, Jaimie; West, Thomas P.; Hughes, Stephen R.; Gibbons, William R.

doi:10.1177/2211068212452873

Cited by 4 publications

(2 citation statements)

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“…We therefore recommend that strain selection and preliminary bioactivity testing should become common practice for all studies focusing on constituents and biological effects of medicinal polypores. Fungal strain selection is routinely applied for a wide range of species used for food (Chiu et al 1999 ; Liu et al 2012 ; Terashima et al 2002 ), biotechnology (Geiger et al 2012 ), and biocontrol (Cui et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Fungal strain matters: colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus

et al. 2015

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Polypores have been applied in traditional Chinese medicine up to the present day, and are becoming more and more popular worldwide. They show a wide range of bioactivities including anti-cancer, anti-inflammatory, antiviral and immuno-enhancing effects. Their secondary metabolites have been the focus of many studies, but the importance of fungal strain for bioactivity and metabolite production has not been investigated so far for these Basidiomycetes. Therefore, we screened several strains from three medicinal polypore species from traditional European medicine: Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus. A total of 22 strains were compared concerning their growth rates, optimum growth temperatures, as well as antimicrobial and antifungal properties of ethanolic fruit body extracts. The morphological identification of strains was confirmed based on rDNA ITS phylogenetic analyses. Our results showed that species delimitation is critical due to the presence of several distinct lineages, e.g. within the Fomes fomentarius species complex. Fungal strains within one lineage showed distinct differences in optimum growth temperatures, in secondary metabolite production, and accordingly, in their bioactivities. In general, F. pinicola and P. betulinus extracts exerted distinct antibiotic activities against Bacillus subtilis and Staphylococcus aureus at minimum inhibitory concentrations (MIC) ranging from 31-125 μg mL−1; The antifungal activities of all three polypores against Aspergillus flavus, A. fumigatus, Absidia orchidis and Candida krusei were often strain-specific, ranging from 125-1000 μg mL−1. Our results highlight that a reliable species identification, followed by an extensive screening for a ‘best strain’ is an essential prerequisite for the proper identification of bioactive material.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-014-0093-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Fungal strain matters: colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus

et al. 2015

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“…Pichia stipitis , a natural xylose-fermenting yeast, has also been used to produce biofuels from cellulosic biomass because it can metabolize various sugars derived from cellulosic biomass without the introduction of any heterologous genes [ 12 - 14 ]. However, wild-type P. stipitis exhibits a significantly slower cellobiose metabolic rate than xylose metabolic rate, indicating that it cannot efficiently ferment mixed sugars from cellulosic biomass without any improvement in cellobiose metabolism [ 15 , 16 ]. Previously, a mutant P. stipitis YN14 strain was constructed by evolutionary engineering through serial subcultures of a wild-type P. stipitis CBS6054 strain in the presence of cellobiose.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Characterization of Mutant Pichia stipitis Co-Fermenting Cellobiose and Xylose through Genomic and Transcriptomic Analyses

Kim

Choi

Lee

et al. 2022

J. Microbiol. Biotechnol.

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The development of a yeast strain capable of fermenting mixed sugars efficiently is crucial for producing biofuels and value-added materials from cellulosic biomass. Previously, a mutant Pichia stipitis YN14 strain capable of co-fermenting xylose and cellobiose was developed through evolutionary engineering of the wild-type P. stipitis CBS6054 strain, which was incapable of cofermenting xylose and cellobiose. In this study, through genomic and transcriptomic analyses, we sought to investigate the reasons for the improved sugar metabolic performance of the mutant YN14 strain in comparison with the parental CBS6054 strain. Unfortunately, comparative wholegenome sequencing (WGS) showed no mutation in any of the genes involved in the cellobiose metabolism between the two strains. However, comparative RNA sequencing (RNA-seq) revealed that the YN14 strain had 101.2 times and 5.9 times higher expression levels of HXT2.3 and BGL2 genes involved in cellobiose metabolism, and 6.9 times and 75.9 times lower expression levels of COX17 and SOD2.2 genes involved in respiration, respectively, compared with the CBS6054 strain. This may explain how the YN14 strain enhanced cellobiose metabolic performance and shifted the direction of cellobiose metabolic flux from respiration to fermentation in the presence of cellobiose compared with the CBS6054 strain.

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Waste to Wealth: The Importance of Yeasts in Sustainable Bioethanol Production from Lignocellulosic Biomass

Watcharawipas

Nanyan

Astuti

2021

Waste Management, Processing and Valorisation

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Evaluation of UV-C Mutagenized Scheffersomyces stipitis Strains for Ethanol Production

Cited by 4 publications

References 22 publications

Fungal strain matters: colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus

Fungal strain matters: colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus

Comprehensive Characterization of Mutant Pichia stipitis Co-Fermenting Cellobiose and Xylose through Genomic and Transcriptomic Analyses

Waste to Wealth: The Importance of Yeasts in Sustainable Bioethanol Production from Lignocellulosic Biomass

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