Evaluation of variant specific trypanolysis tests for serodiagnosis of human infections with Trypanosoma brucei gambiense

Meirvenne, N Van; Magnus, E; Büscher, P.

doi:10.1016/0001-706x(95)00127-z

Cited by 112 publications

(109 citation statements)

References 15 publications

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“…The CATT/T. evansi and the TL assay were carried out as described by Verloo et al (24) and Van Meirvenne et al (21), respectively. The CATT/T.…”

Section: Methodsmentioning

confidence: 99%

“…Lysis of the trypanosomes occurs when RoTat 1.2-specific antibodies are present in the test serum. The TL assay is considered the gold standard for the presence of specific antibodies against African trypanosomes (21,23).…”

Section: Methodsmentioning

confidence: 99%

“…Indirect diagnosis is possible through detection of specific antibodies in the mammalian hosts. All currently available antibody detection tests, including an immune trypanolysis (TL) assay (21), an enzymelinked immunosorbent assay for T. evansi (ELISA/T. evansi) (22), a card agglutination test for trypanosomiasis for T. evansi (CATT/T.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Towards a New Reference Test for Surra in Camels

Tran

Claes

Verloo

et al. 2009

Clin Vaccine Immunol

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“…The CATT/T. evansi and the TL assay were carried out as described by Verloo et al (24) and Van Meirvenne et al (21), respectively. The CATT/T.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Towards a New Reference Test for Surra in Camels

Tran

Claes

Verloo

et al. 2009

Clin Vaccine Immunol

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“…21 The status of 74 individuals included in the study was defined using serologic (card agglutination trypanosoma tests for T. b .gambiense and trypanolysis test), parasitologic, and molecular (polymerase chain reaction) investigations. 22,23 Stage diagnosis was carried out for all sleeping sickness cases (n ‫ס‬ 19), by counting white blood cells present in the cerebrospinal fluid (CSF). These results led to defining three groups of individuals: 1) the ENI group, which included exposed but non-infected individuals (n ‫ס‬ 55) who had no positive test results; 2) the P1 group, which included exposed and infected patients in the first stage of the disease (n ‫ס‬ 7) who had positive test results and < 5 white blood cells/L in CSF; and 3) the P2 group, which included exposed and infected patients in the second stage of the disease (n ‫ס‬ 12) who had positive test results and Ն 5 white blood cells/l in the CSF.…”

Section: Methodsmentioning

confidence: 99%

Human/Vector Relationships During Human African Trypanosomiasis: Initial Screening of Immunogenic Salivary Proteins of Glossina Species

Poinsignon¹,

Cornélie²,

Remoué³

et al. 2007

The American Journal of Tropical Medicine and Hygiene

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Abstract. The morbidity and mortality of vector-borne diseases is closely linked to exposure of the human host to vectors. Qualitative and quantitative evaluation of individual exposure to arthropod bites by investigation of the specific immune response to vector saliva would make it possible to monitor individuals at risk of vectorial transmission of pathogens. The objective of this study was to evaluate and compare the antibody (IgG) response to saliva from uninfected Glossina species, vectors, or non-vectors of Trypanosoma brucei gambiense by detecting immunogenic proteins in humans residing in an area endemic for human African trypanosomiasis in the Democratic Republic of Congo. Our results suggest that the immunogenic profiles observed seemed specific to the Glossina species (vector or non-vector species) and to the infectious status of exposed individuals (infected or not infected). This preliminary work tends to support the feasibility of development of an epidemiologic tool based on this antibody response to salivary proteins.

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“…One of the predominant VSGs of Trypanosoma brucei gambiense, causing the chronic form of sleeping sickness or human African trypanosomiasis (HAT), is VSG LiTat 1.5 (Van Meirvenne et al, 1995). All currently available antibody detection tests for gambiense HAT, i.e., card agglutination test for trypanosomiasis (CATT) (Magnus et al, 1978), LATEX/T.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

Nieuwenhove

Rogé²,

Lejon³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. At present, all available diagnostic antibody detection tests for Trypanosoma brucei gambiense human African trypanosomiasis are based on predominant variant surface glycoproteins (VSGs), such as VSG LiTat 1.5. During investigations aiming at replacement of the native VSGs by recombinant proteins or synthetic peptides, the sequence of VSG LiTat 1.5 was derived from cDNA and direct N-terminal amino acid sequencing. Characterization of the VSG based on cysteine distribution in the amino acid sequence revealed an unusual cysteine pattern identical to that of VSG Kinu 1 of T. b. brucei. Even though both VSGs lack the third of four conserved cysteines typical for type A N-terminal domains, they can be classified as type A.

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Evaluation of variant specific trypanolysis tests for serodiagnosis of human infections with Trypanosoma brucei gambiense

Cited by 112 publications

References 15 publications

Towards a New Reference Test for Surra in Camels

Towards a New Reference Test for Surra in Camels

Human/Vector Relationships During Human African Trypanosomiasis: Initial Screening of Immunogenic Salivary Proteins of Glossina Species

Short Communication Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

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