2021
DOI: 10.1101/2021.02.19.21252059
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Evaluation of VIDAS® diagnostic assay prototypes detecting dengue virus NS1 antigen and anti-dengue virus IgM and IgG antibodies

Abstract: Dengue is a tropical disease caused by the mosquito-borne dengue virus (DENV) and affecting an estimated 96 million people each year. Performant, rapid and easy-to-use assays are needed for the accurate diagnosis of acute DENV infection. This study evaluated the performance of three prototype assays developed for the VIDAS automated platform to detect dengue NS1 antigen and anti-dengue IgM and IgG antibodies. Positive and negative agreement with competitor enzyme-linked immunosorbent assays (ELISA) and rapid d… Show more

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Cited by 3 publications
(9 citation statements)
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“…Antibodies are glycoproteins involved in the pathogenesis of autoimmune diseases, which play a critical role in immunopathology [1]. The past years have witnessed the detection of antibodies as an important approach for the diagnosis of several diseases such as HIV [2], dengue [3], Zika virus [4] and autoimmune diseases [5]. Particularly, due to the fast global spread of coronavirus disease (COVID-19) from the end of 2019, assay of immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies for evaluating the diagnostic accuracy is especially important for COVID-19 patients [6].…”
Section: Introductionmentioning
confidence: 99%
“…Antibodies are glycoproteins involved in the pathogenesis of autoimmune diseases, which play a critical role in immunopathology [1]. The past years have witnessed the detection of antibodies as an important approach for the diagnosis of several diseases such as HIV [2], dengue [3], Zika virus [4] and autoimmune diseases [5]. Particularly, due to the fast global spread of coronavirus disease (COVID-19) from the end of 2019, assay of immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies for evaluating the diagnostic accuracy is especially important for COVID-19 patients [6].…”
Section: Introductionmentioning
confidence: 99%
“…The VIDAS ® DENGUE NS1 Ag (DEAG; 423077), VIDAS ® Anti-DENGUE IgM (DENM; 423078) and VIDAS ® Anti-DENGUE IgG (DENG; 423079) assays (bioMérieux SA, Marcy-l'Étoile, France) are automated qualitative two-step immunoassays developed for VIDAS ® instruments [24]. The VIDAS ® DENGUE NS1 Ag assay detects the dengue NS1 antigen of the four DENV serotypes.…”
Section: Vidas ® Assaysmentioning
confidence: 99%
“…The VIDAS ® Anti-DENGUE IgM and IgG assays detect IgM and IgG antibodies, respectively, recognising antigens of the four DENV serotypes, owing to the use of a recombinant tetravalent EDIIIT2 protein composed of the antigenic DENV-specific envelope domain III of the four DENV serotypes [24,27]. The three VIDAS ® dengue assays were performed and interpreted according to the instructions for use, as previously described [24]. A test was interpreted as negative when the index value (i) was < 1.0 and positive when i ≥ 1.0.…”
Section: Vidas ® Assaysmentioning
confidence: 99%
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