Evaluation of virulence of Mycoplasma hyopneumoniae field isolates

Vicca, Jo; Stakenborg, Tim; Maes, Dominiek; Butaye, Patrick; Peeters, Jan; Kruif, Aart de; Haesebrouck, Freddy

doi:10.1016/j.vetmic.2003.08.008

Cited by 105 publications

(94 citation statements)

References 15 publications

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“…We show that (i) M. hyopneumoniae adheres intimately to the surface of PK15 cells where microvilli predominate, (ii) heparin blocks the ability of this pathogen to adhere to PK15 cells, and (iii) regions within P159 are intimately involved in adherence to PK15 cells. Despite possessing limited coding capacity, evidence of differences in strain virulence (Vicca et al, 2003), cleavage of key surface proteins (Djordjevic et al, 2004), and our recent report that key surface proteins bind glycosaminoglycans (Jenkins et al, 2006) suggests that subtle changes in surface protein sequences may have significant ramifications for disease caused by M. hyopneumoniae.…”

Section: Discussionmentioning

confidence: 98%

P159 is a proteolytically processed, surface adhesin ofMycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells

Burnett

Dinkla

Rohde

et al. 2006

Molecular Microbiology

185

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SummaryMycoplasma hyopneumoniae , the causative agent of porcine enzootic pneumonia, colonizes the respiratory cilia of affected swine causing significant economic losses to swine production worldwide. Heparin is known to inhibit adherence of M. hyopneumoniae to porcine respiratory epithelial cilia. M. hyopneumoniae cells bind heparin but the identity of the heparinbinding proteins is limited. Proteomic analysis of M. hyopneumoniae lysates identified 27 kDa (P27), 110 kDa (P110) and 52 kDa (P52) proteins representing different regions of a 159 kDa (P159) protein derived from mhp494. These cleavage fragments were surface located and present at all growth stages.

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Section: Discussionmentioning

confidence: 98%

P159 is a proteolytically processed, surface adhesin ofMycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells

Burnett

Dinkla

Rohde

et al. 2006

Molecular Microbiology

185

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“…This indicates that different M. hyopneumoniae strains are circulating in the swine population. The occurrence of different strains has been demonstrated by others as well [1,4,14]. Classical typing methods described for M. hyopneumoniae like amplified-fragmentlength-polymorphism (AFLP) or pulsedfield-gel-electrophoresis (PFGE) are cumbersome and generally require isolation and cultivation of M. hyopneumoniae [8,9].…”

Section: Introductionmentioning

confidence: 99%

Diversity ofMycoplasma hyopneumoniaein pig farms revealed by direct molecular typing of clinical material

et al. 2007

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-Mycoplasma hyopneumoniae is the etiological agent of enzootic pneumonia in swine.Various reports indicate that different strains are circulating in the swine population. We investigated the variety of M. hyopneumoniae strains by a newly developed genetic typing method based on the polyserine repeat motif of the LppS homolog P146. PCR amplification using M. hyopneumoniae specific, conserved primers flanking the region encoding the repeat motif, followed by sequencing and cluster analysis was carried out. The study included strains isolated from different geographic regions as well as lysates from lung swabs from a series of pig farms in Switzerland. High diversity of M. hyopneumoniae was observed but farms being in close geographic or operative contact generally seemed to be affected by the same strains. Moreover, analysis of multiple samples from single pig farms indicated that these harbored the same, farm-specific strain. The results indicate that multiple strains of M. hyopneumoniae are found in the swine population but that specific strains or clones are responsible for local outbreaks. The method presented is a highly reproducible epidemiologic tool allowing direct typing of M. hyopneumoniae from clinical material without prior isolation and cultivation of strains. enzootic pneumonia / epidemiology / LppS / P146

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“…VICCA et al (2003) encontraram uma alta variação na virulência de cepas de M. hyopneumoniae isoladas em diferentes rebanhos. As cepas de moderada ou alta virulência apresentaram uma banda de 5000 pb, determinada através de randomly amplified polymorphic DNA (RAPD), que estava ausente nas cepas de baixa virulência.…”

Section: Etiopatogeniaunclassified