Physio-biochemical response and phytochemical variation in three West Asian genotypes (Torbetjam, Izmir and Gaziantep) of fennel (Foeniculum vulgare Mill) have been investigated in green house conditions to study the impact of water stress at 100, 50 and 35% of field capacity. In the Torbetjam genotype, chlorophyll content, plant growth and yield parameters under intense drought condition were slightly reduced, but content of compatible solutes (proline and glycine betaine) was increased by 36 and 47% as compared to the control. Drought stress promoted enzymatic and non-enzymatic antioxidant system in all of the fennel genotypes. Torbetjam genotype exhibited more antioxidant enzyme activity, and the concentration of antioxidants like anthocyanins, tannins and flavonoids was increased by 127, 73 and 25% compared with control. Under drought stress, Torbetjam genotype resulted in higher monoterpenic constituents like limonene and betapinene and fewer stress markers like malondialdehyde and hydrogen peroxide, therefore causing less cellular membrane damage and lipid peroxidation. Based on chemometrical analysis, Torbetjam genotype can be considered as a droughttolerant genotype.