Evaluation of Watermelon and Related Germplasm for Resistance to Zucchini Yellow Mosaic Virus

Boyhan, George E.; Norton, J. D.; Jacobsen, Barry J.; Abrahams, B.R.

doi:10.21273/hortsci.27.11.1173a

Cited by 13 publications

(19 citation statements)

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“…Genes pertaining to viral resistance have been identified in several cucurbits (Munger 1993;Provvidenti 1993;Provvidenti and Gonsalves 1982), such as Citrullus sp. gemplasm for resistance to Watermelon mosaic virus 2, (Gillaspie and Wright 1993;Xu et al 2004), Papaya ringspot virus W (PRSV W) (Strange et al 2002) or Zucchini yellows mosaic virus (ZYMV) (Provvidenti 1991;Boyhan et al 1992;Xu et al 2004). The use of traditional breeding mechanisms to introduce resistant genes into commercial cultivars, when used in removing unfavorable traits and attempting to find material sources that are genetically agreeable (Compton and Gray 1993b), is very inefficient and wastes a lot of time.…”

Section: Introductionmentioning

confidence: 99%

Transgenic watermelon lines expressing the nucleocapsid gene of Watermelon silver mottle virus and the role of thiamine in reducing hyperhydricity in regenerated shoots

Huang

Chiang

et al. 2010

Plant Cell Tiss Organ Cult

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Viral diseases are very detrimental to watermelon production. Watermelon silver mottle virus (WSMoV) is a major limiting factor for the production of watermelon and other cucurbit fruits. There are no effective natural sources of resistance to WSMoV, making transgenic resistance an appropriate solution for attenuating virus infection. Hyperhydricity is an important problem in watermelon culture in vitro, resulting from lower multiplication rates, poor quality shoots and tissue necrosis. In this study, we report an Agrobacterium-mediated genetic transfer protocol for commercial watermelon cultivars expressing the nucleocapsid (N) gene of WSMoV and a suitable approach to overcome hyperhydricity in watermelon culture in vitro. Murashige and Skoog (MS) salts containing Schenk and Hildebrandt (SH) vitamins ? 50 mg l -1 thiamine HCl could diminish the hyperhydric phenotype. The proximal halves of cotyledons from 3-dayold seedlings were cut into 1.5 9 1.5 mm segments as explants. Four days after co-cultivation, the explants were transferred to a selection medium for shoot regeneration. The putative transgenic shoots developed within 6 weeks of culture and were then transferred to stringent medium for 8 weeks to eliminate 'escape type' shoots. Fifty putative transgenic watermelon lines were obtained from three cultivars. PCR and Southern blot analysis confirmed that the foreign gene was incorporated into the genomic DNA of the transgenic lines.

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Section: Introductionmentioning

confidence: 99%

Transgenic watermelon lines expressing the nucleocapsid gene of Watermelon silver mottle virus and the role of thiamine in reducing hyperhydricity in regenerated shoots

Huang

Chiang

et al. 2010

Plant Cell Tiss Organ Cult

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“…By using pathogen-resistant or abiotic stress-resistant cultivars watermelon yields can be increased. The cultivars have been developed by traditional breeding to have resistance to watermelon fruit blotch (Rane and Latin 1992), to watermelon mosaic virus (Gillaspie and Wright 1993), and to zucchini yellows mosaic virus (Boyhan et al 1992). Recent advances in insertion of bacterial, fungal and virus resistance genes through Agrobacterium-mediated transformation would facilitate the development of new disease resistant genotypes without significantly altering the genetic composition, and have made it possible to improve their productivity and quality beyond the limit of traditional breeding.…”

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confidence: 99%

Agrobacterium-mediated transformation in Citrullus lanatus

Cho¹,

Moon²,

Liu³

et al. 2008

Biologia plant.

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Agrobacterium tumefaciens-mediated transformation was used to produce transgenic watermelon. Cotyledonary explants of Citrullus lanatus Thumb (cv. Daesan) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing pPTN289 carrying with bar gene and pPTN290 carrying with nptII gene, respectively. There was a significant difference in the transformation frequency between bacteria strains and selective markers. The EHA101/pPTN289 showed higher transformation frequency (1.16 %) than GV3101/pPTN289 (0.33 %) and LBA4404/pPTN289 or /pPTN290 (0 %). The shoots obtained (633 and 57 lines) showed some resistance to glufosinate and paromomycin, respectively. Of them, the β-glucuronidase positive response and PCR products amplified by bar and nptII specific primers showed at least 21 plants resistant to glufosinate and at least 6 plants to paromomycin. Southern blot analysis revealed that the bar gene integrated into genome of transgenic watermelon. Acclimated transgenic watermelons were successfully transplanted in the greenhouse and showed no phenotypic variation.

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“…'AU-Performance' was developed as an improved version of 'AU-Producer' with fungal and ZYMV resistance. The resistant parent of 'AU-Performance', PI595203, was shown to be resistant to PRSV, WMV, and ZYMV (Boyhan et al, 1992;Gillaspie and Wright, 1993;Strange et al, 2002;Xu et al, 2004). Our data show that 'AU-Performance' is not resistant to PRSV, WMV, or ZYMV.…”

Section: Resultsmentioning

confidence: 64%

“…Only four of the PIs were resistant to ZYMV and the resistance was inherited as a single recessive gene designated zym. Boyhan et al (1992) evaluated 153 PIs, breeding lines, and commercial varieties for resistance to the Florida strain of ZYMV (ZYMV-FL). They identified five sources of resistance, and included among them was PI595203 (also referred to as 'Egun').…”

mentioning

confidence: 99%

“…Norton. The discovery of ZYMV resistance in 'Egun' (Boyhan et al, 1992) led to the continuation of breeding efforts to introgress the ZYMV resistance of PI595203 ('Egun') into 'AU-Producer'. A cross between PI595203 and 'AU-Producer' was used, followed by four backcross generations and field selections for diseaseresistant seedlings that produced high yields of excellent fruit quality (Boyhan et al, 2001(Boyhan et al, , 2002(Boyhan et al, , 2000.…”

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confidence: 99%

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Evaluation of ‘AU-Performance’ Watermelon for Its Response to Virus Inoculation

Murphy

Dane

2009

hortte

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The watermelon (Citrullus lanatus var. lanatus) ‘AU-Performance’ was developed for resistance to multiple fungal pathogens and the plant virus, zucchini yellow mosaic virus (ZYMV). A greenhouse-based evaluation was carried out to determine the response of ‘AU-Performance’ to inoculation with three important cucurbit (Cucurbitaceae) viruses in the genus Potyvirus: papaya ringspot virus (PRSV), watermelon mosaic virus (WMV), and ZYMV. The evaluation included the resistant parent (PI595203), the susceptible parent (‘AU-Producer’), and varieties AU-Allsweet and Charleston Gray. Each of the three viruses systemically infected ‘AU-Performance’ with 100% infection and development of characteristic systemic symptoms. The susceptible parent (‘AU-Producer’), ‘AU-Allsweet’, and ‘Charleston Gray’ responded similarly with 100% infection and systemic symptoms. In contrast, the resistant parent (PI595203) was resistant to WMV and ZYMV; however, PRSV-inoculated plants developed a systemic infection with accompanied symptoms and high levels of PRSV accumulation in noninoculated leaves. PI595203 was shown in previous studies to be resistant to PRSV. We show in this report that under greenhouse conditions and application of virus by mechanical inoculation, ‘AU-Performance’ was not resistant to infection by the three potyviruses.

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Evaluation of Watermelon and Related Germplasm for Resistance to Zucchini Yellow Mosaic Virus

Cited by 13 publications

References 0 publications

Transgenic watermelon lines expressing the nucleocapsid gene of Watermelon silver mottle virus and the role of thiamine in reducing hyperhydricity in regenerated shoots

Transgenic watermelon lines expressing the nucleocapsid gene of Watermelon silver mottle virus and the role of thiamine in reducing hyperhydricity in regenerated shoots

Agrobacterium-mediated transformation in Citrullus lanatus

Evaluation of ‘AU-Performance’ Watermelon for Its Response to Virus Inoculation

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