2020
DOI: 10.1016/j.pupt.2020.101940
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Evaluation of waterpipe smoke toxicity in C57BL/6 mice model

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Cited by 7 publications
(2 citation statements)
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“…Harvested organs (lungs and liver) from 6 CON and 7 GE mice were fixed in 10% neutral buffered formalin for 48 h, dehydrated in 95% ethanol, and then embedded in paraffin as described previously (Abi‐Gerges et al, 2020; Zeeni et al, 2015). Briefly, two different sections of 5 μm thickness each were obtained from each organ and stained with H&E. All slides were evaluated blindly for pathological changes by an experienced board‐certified pathologist under a light microscope (Zeiss).…”
Section: Methodsmentioning
confidence: 99%
“…Harvested organs (lungs and liver) from 6 CON and 7 GE mice were fixed in 10% neutral buffered formalin for 48 h, dehydrated in 95% ethanol, and then embedded in paraffin as described previously (Abi‐Gerges et al, 2020; Zeeni et al, 2015). Briefly, two different sections of 5 μm thickness each were obtained from each organ and stained with H&E. All slides were evaluated blindly for pathological changes by an experienced board‐certified pathologist under a light microscope (Zeiss).…”
Section: Methodsmentioning
confidence: 99%
“…Recent research revealed the presence of multiple dangerous toxins and carcinogens in its smoke and the lack of their filtration through the water. In addition, a study examining the underlying risks of subchronic WP exposure in mice showed increased urea serum levels, lungs, kidneys, bone marrow, and liver damage compared to controls [11]. In previous studies, a significant association of WPS with lung cancer was established; gastric and esophageal cancers were observed to have weak associations with WPS [12].…”
Section: Introductionmentioning
confidence: 99%