Evaluation of Wedged Arterial Injection as a New Technique for Delivery of Experimental Therapeutic Sustances into the Porcine Pancreas

Abstract

Objectives. To prospectively evaluate the technical feasibility and efficacy of wedged arterial injection (WAI) as a potential route for experimental selective therapy to the pancreas of healthy pigs. Materials and Methods. Selective angiographies were completed in ten pigs under general anaesthesia. By superselective angiography, the catheter was inserted and wedged into the major pancreatic artery, blocking the blood flow. In order to evaluate the efficacy of the WAI method, a DNA-specific fluorescent dye (Hoechst 33258) was used. Results. Histological study revealed a uniform distribution of the fluorescent dye within the nuclei of the endocrine and exocrine pancreatic cells. Pancreatic and liver enzymes as well as histopathology of the pancreas were normal. Conclusion. WAI is a highly effective minimally invasive methodology to target the porcine pancreas. The findings suggest that WAI may contribute to developing preclinical assays of pancreas gene or cell-transfer therapies in swine model.

Evidences supporting the vascular etiology of post-double balloon enteroscopy pancreatitis: Study in porcine model

“…By road-map guidance, the cobra catheter was selectively positioned and wedged into the major pancreatic artery. The super-selective angiography of this artery was conducted by manual injection of 3-5 mL contrast medium to ensure that the tip of the catheter was at the desired site[ 53 ]. The nuclear dye Hoechst (Bizbenzimida H 33258 fluorochrome) was injected through that vessel to allow its contact with the cells in the tail of the pancreas (Figure 5 ).…”