Evaluation of Weeds as Virus Reservoirs in Watermelon Crops

Aguiar, Raimundo Wagner Souza; Alves, Giselly Batista; Queiroz, A. P. de; Nascimento, Ildon Rodrigues do; Lima, M. F.

doi:10.1590/s0100-83582018360100032

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…The ecology of begomoviruses identified by HTS studies in non-cultivated plants requires more effort in order to understand the contribution of these plants for disease development [74,75,76]. Non-cultivated plants could be a source of viral inoculum to cultivated plants [77,78,79,80,81,82,83] and could contribute to viral evolution. Here, we described some non-cultivated plants at the agro-ecological interface carrying geminivirus-signatures (Table 3 and Supplementary Table S2).…”

Section: Resultsmentioning

confidence: 99%

High-Throughput Sequencing Reveals Differential Begomovirus Species Diversity in Non-Cultivated Plants in Northern-Pacific Mexico

Rodríguez-Negrete

Morales-Aguilar

Domínguez-Durán

et al. 2019

Viruses

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Plant DNA viruses of the genus Begomovirus have been documented as the most genetically diverse in the family Geminiviridae and present a serious threat for global horticultural production, especially considering climate change. It is important to characterize naturally existing begomoviruses, since viral genetic diversity in non-cultivated plants could lead to future disease epidemics in crops. In this study, high-throughput sequencing (HTS) was employed to determine viral diversity of samples collected in a survey performed during 2012–2016 in seven states of Northern-Pacific Mexico, areas of diverse climatic conditions where different vegetable crops are subject to intensive farming. In total, 132 plant species, belonging to 34 families, were identified and sampled in the natural ecosystems surrounding cultivated areas (agro-ecological interface). HTS analysis and subsequent de novo assembly revealed a number of geminivirus-related DNA signatures with 80 to 100% DNA similarity with begomoviral sequences present in the genome databank. The analysis revealed DNA signatures corresponding to 52 crop-infecting and 35 non-cultivated-infecting geminiviruses that, interestingly, were present in different plant species. Such an analysis deepens our knowledge of geminiviral diversity and could help detecting emerging viruses affecting crops in different agro-climatic regions.

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Section: Resultsmentioning

confidence: 99%

High-Throughput Sequencing Reveals Differential Begomovirus Species Diversity in Non-Cultivated Plants in Northern-Pacific Mexico

Rodríguez-Negrete

Morales-Aguilar

Domínguez-Durán

et al. 2019

Viruses

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“…More recently, a study observed the interactions between P. angulate and several cucurbit viruses, including watermelon mosaic virus and zucchini yellow mosaic virus. It was revealed that this weed was a host of several viruses, and served as a secondary source of infection for cucurbits [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the ability of these weeds, and others that are known hosts, to successfully transmit the virus to other Cucurbitaceae hosts has not been well documented. Previous research in Brazil in watermelon growing regions has identified several weeds, including A. spinosus , P. angulate, and Heliotrpoium indicum , as potential reservoirs for viruses of a range of genera, including potyviruses, cucumoviruses, and orthotospoviruses [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Ability of Non-Hosts and Cucurbitaceous Weeds to Transmit Cucumber Green Mottle Mosaic Virus

Lovelock

Mintoff²,

Kurz³

et al. 2023

Viruses

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Cucumber green mottle mosaic virus (CGMMV) is a Tobamovirus of economic importance affecting cucurbit crops and Asian cucurbit vegetables. Non-host crops of CGMMV, including capsicum (Capsicum annum), sweetcorn (Zea mays), and okra (Abelmoschus esculentus), were tested for their susceptibility to the virus, with field and glasshouse trials undertaken. After 12 weeks post-sowing, the crops were tested for the presence of CGMMV, and in all cases, no CGMMV was detected. Commonly found within the growing regions of cucurbits and melons worldwide are weeds, such as black nightshade (Solanum nigrum), wild gooseberry (Physalis minima), pigweed (Portulaca oleracea), and Amaranth species. Several weeds/grasses were tested for their ability to become infected with CGMMV by inoculating weeds directly with CGMMV and routinely testing over a period of eight weeks. Amaranthus viridis was found to be susceptible, with 50% of the weeds becoming infected with CGMMV. To further analyse this, six Amaranth samples were used as inoculum on four watermelon seedlings per sample and tested after eight weeks. CGMMV was detected in three of six watermelon bulk samples, indicating that A. viridis is a potential host/reservoir for CGMMV. Further research into the relationship between CGMMV and weed hosts is required. This research also highlights the importance of proper weed management to effectively manage CGMMV.

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“…For instance, PRSV-W was detected in 22% of symptomatic plants by ELISA method, while mRT-PCR found it in 75% (Table 2). It is worth mentioning that weeds can act as natural reservoirs of these watermelon viruses, particularly ZYMV and PRSV-W (Aguiar et al, 2018). The distribution of virus in the state can be attributed to the mode of transmission, nonpersistent by several species of aphids, of these viruses (CMV, PRSV-W, WMV, and ZYMV).…”

Section: Discussionmentioning

confidence: 99%

Multiplex RT-PCR identification of five viruses associated with the watermelon crops in the Brazilian Cerrado

Aguiar¹,

Martins²,

Nascimento³

et al. 2019

Afr. J. Microbiol. Res.

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Watermelon plants can be naturally infected by several viruses in single or mixed infections, of which the diagnosis is difficult and require specific techniques. This study aims to detect and to verify the presence of Cucumber mosaic virus (CMV), Papaya ringspot virus type W (PRSV-W), Watermelon mosaic virus (WMV), Zucchini lethal chlorosis virus (ZLCV), Zucchini yellow mosaic virus (ZYMV), and the main cucurbit viruses in Brazil using multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay. Oligonucleotides were designed towards the conserved regions of the virus genomes. In the duplex-PCR, it was possible to detect all the virus combinations, except ZLCV with ZYMV. The amplified product sizes were 644 bp (CMV), 535 bp (WMV-2), 398 bp (PRSV-W), 244 bp (ZLCV), and 214 bp (ZYMV). To test the efficacy of the methodology, we analyzed plants with virus symptoms from four municipalities in the state of Tocantins, located at Brazilian Cerrado. Mixed infections were detected in 80% of samples in all the municipalities. The multiplex RT-PCR assay can be used to detect and differentiate watermelon viruses that affect crops in the state of Tocantins.

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Evaluation of Weeds as Virus Reservoirs in Watermelon Crops

Cited by 8 publications

References 11 publications

High-Throughput Sequencing Reveals Differential Begomovirus Species Diversity in Non-Cultivated Plants in Northern-Pacific Mexico

High-Throughput Sequencing Reveals Differential Begomovirus Species Diversity in Non-Cultivated Plants in Northern-Pacific Mexico

Ability of Non-Hosts and Cucurbitaceous Weeds to Transmit Cucumber Green Mottle Mosaic Virus

Multiplex RT-PCR identification of five viruses associated with the watermelon crops in the Brazilian Cerrado

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