Evaluation of whole blood collected on to paper discs for the sero-diagnosis of Aujeszky's disease by ELISA

A one-step immunochromatographic test, based on the use of monoclonal antibodies, was developed for the detection of canine parvovirus (CPV) in dog faeces. In addition to canine parvovirus the test can also be used for the diagnosis of infections with viruses causing parvovirus enteritis in cats (feline panleukopenia virus) and mink (mink enteritis virus). Four hundred and forty-three faecal samples were evaluated by comparative testing between this one-step test and three different enzyme-linked immunosorbent assays (ELISA) in Sweden, Denmark and The Netherlands. The result of the evaluation showed an overall relative sensitivity and specificity of 95.8 and 99.7%, respectively. Furthermore, the comparative testing of 83 dog samples in Germany between the one-step test and an immune electron microscopy (IEM) agreed to 85.5%. The sensitivity and specificity were 83.9 and 88.9%, respectively. These results show that the one-step test is a rapid, simple, reproducible and sensitive diagnostic test for the detection of parvovirus in faecal samples of dogs, cats and mink.

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“…Agreement, relative sensitivity and specificity of the one‐step parvovirus test were calculated according to Motha et al . (1987).…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of a New Rapid and One‐Step Test for the Detection of Parvovirus in Faeces from Dogs, Cats and Mink

Esfandiari

Klingeborn

2000

Journal of Veterinary Medicine, Series B

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“…In 1987, two major modifications were introduced to the ELISA, mainly to increase its sensitivity and specificity (Banks and Cartwright 1983), and to simplify the sample collection process by introducing the ELISA disc technique (Banks 1985). Motha and others (1987) used the modified test procedures and compared the ELISA disc technique for sample collection with serum samples collected from the same pigs; there was 100 per cent agreement in the results. For all subsequent surveys in New Zealand, the ELISA disc technique for sample collection and the modified test procedures were used.…”

Section: Methodsmentioning

confidence: 99%

“…To supplement this continuous surveillance, another serological survey of the South Island was carried out in 1993 (McLaughlin 1995). All culled breeding pigs from nine abattoirs representing all regions of the South Island were tested by using the ELISA disc technique (Motha and others 1987).…”

Section: Methodsmentioning

confidence: 99%

Eradication of Aujeszky's disease from New Zealand pig herds 1976–1997

Pannett¹,

Motha²,

MacDiarmid

1999

Veterinary Record

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Aujeszky's disease was first diagnosed in the North Island of New Zealand in 1976. It has never been reported in the South Island. An industry-funded eradication programme was initiated in 1989 to eradicate the disease from the national pig herd. By using a combination of serological surveys, abattoir surveillance, test and slaughter, depopulation, vaccination and movement restrictions, Aujeszky's disease was eradicated by 1997.

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“…The enzyme reaction was stopped after 10 min by adding 50 l of 5 M H 2 SO 4 , and the optical density (OD) was read at 450 nm with a spectrophotometer. d The results were interpreted by calculating the percent inhibition (PI) according to the following formula: 10 PI ϭ [(negative control OD Ϫ sample OD) Ϭ negative control OD] ϫ 100. All sera with a PI Ͼ35 are considered true positive, and all with a PI Ͻ25 are considered true negatives.…”

Section: B-elisa Reagents and Proceduresmentioning

confidence: 99%

Development of a Blocking ELISA for Screening Antibodies to Porcine Rubulavirus, La Piedad Michoacan Virus

et al. 1999

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Abstract.A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to porcine rubulavirus (La Piedad Michoacan Virus [LPMV]) in serum samples from pigs. The test, based on a monoclonal antibody against the LPMV hemagglutinin-neuraminidase glycoprotein, had a sensitivity of 99% and a specificity of 97%. The results of this test were in agreement with those obtained by an indirect ELISA and hemagglutination inhibition, indirect immunofluorescence, and virus neutralization tests. The blocking ELISA is considered the most suitable test for routine screening for antibodies against LPMV.

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Evaluation of whole blood collected on to paper discs for the sero-diagnosis of Aujeszky's disease by ELISA

Cited by 13 publications

References 2 publications

A Comparative Study of a New Rapid and One‐Step Test for the Detection of Parvovirus in Faeces from Dogs, Cats and Mink

A Comparative Study of a New Rapid and One‐Step Test for the Detection of Parvovirus in Faeces from Dogs, Cats and Mink

Eradication of Aujeszky's disease from New Zealand pig herds 1976–1997

Development of a Blocking ELISA for Screening Antibodies to Porcine Rubulavirus, La Piedad Michoacan Virus

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