Evaluation of whole cell and subcellular vaccines against Brucella ovis in rams

Blasco, J.M.; Gamazo, Carlos; Winter, A J; Bagüés, María Pilar Jiménez de; Marín, C. M.; Barberán, M.; Moriyón, Ignacio; Alonso-Urmeneta, B.; Díaz, Ramón

doi:10.1016/0165-2427(93)90198-d

Cited by 46 publications

(18 citation statements)

References 26 publications

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“…Therefore, we believed that one or more HS antigens, particularly the surface-exposed group 3 protein, might induce a protective immune response to B. ovis. In fact, we have preliminary evidence that HS incorporated with an adjuvant induced significant levels of protection in rams against B. ovis (8).…”

mentioning

confidence: 76%

Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines

et al. 1994

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Experiments were performed with BALB/c mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to Brucella ovis and to compare infection immunity with immunity developed through vaccination with a hot saline extract (HS) of B. ovis. Mice convalescing from a primary infection with B. ovis displayed a high level of resistance to reinfection, as evidenced by splenic bacterial counts decreased over 10,000-fold from control groups at 2 weeks after challenge. Passive transfer assays revealed that protection was mediated by both T lymphocytes and antibodies but that antibodies had a substantially greater role on the basis of log units of protection that were transferred. Antibodies specific for HS proteins in sera from convalescent mice were predominantly of the immunoglobulin G 2a and 3 isotypes. Vaccination with HS conferred good protection against B. ovis, but protection was greatly enhanced by the incorporation of QS-21 or other adjuvants. Protection provided by the HS vaccine resulted largely from immune responses to its protein moieties. A critical evaluation of the protective efficacy of the rough lipopolysaccharide component of HS was precluded by its poor immunogenicity in BALB/c mice. HS-QS-21 afforded protection against challenge infection with B. ovis as good as that which developed after a primary infection and as good as or better than that provided by attenuated Brucella melitensis vaccine strain Rev 1. Passive transfer experiments confirmed that the magnitudes of both humoral and cell-mediated forms of protective immunity were equivalent in mice vaccinated with HS-QS-21 and those recovering from a primary infection. Protective immunity to B. ovis in mice therefore resembled that to Brucella abortus, except that the relative roles of humoral and cell-mediated immunity, rather than being equivalent, were shifted toward a greater role for antibodies.

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confidence: 76%

Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines

et al. 1994

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“…Bacterial antigens were obtained by HS extraction or formalin-inactivation (bacterins) from SEΔ gal (HS-SEΔ gal and B-SEΔ gal ), SEΔ waaL (HS-SEΔ waaL and B-SEΔ waaL ) and SE-wt (HS-SEwt and B-SEwt) strains, as described previously [19,34]. …”

Section: Methodsmentioning

confidence: 99%

The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

Román

Garrido

Muñoz

et al. 2012

Vet Res

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The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking O-PS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella Enteritidis rough mutants, carrying either intact (SEΔwaaL) or deep-defective (SEΔgal) LPS-Core, were used in combination with EDA. Biotinylated bacterins, in particular SEΔwaaL bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from Salmonella Enteritidis lacking O-PS and the usefulness of BEDA-SEΔwaaL as non-live vaccine in the mouse model.

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“…Esto, junto con su elevada respuesta inmune, efectiva protección y no virulencia, convierte a estas nanopartículas en una vacuna ideal anti Brucelosis. Brucella ovis presenta una elevada inmunogenicidad 9,10 . Sin embargo, debido a la naturaleza no replicante del antígeno se requiere el empleo de adyuvantes adecuados, tales como nanopartículas poliméricas biodegradables y bioadhesivas que incrementen la respuesta inmune.…”

Section: Radiomarcaje Y Estudios De Biodistribución De Nanopartículasunclassified

Radiomarcaje y estudios de biodistribución de nanopartículas poliméricas como adyuvantes para la vacunación oftálmica frente a la brucelosis

Sánchez-Martínez

Martins

Quincoces

et al. 2013

Revista Española de Medicina Nuclear e Imagen Molecular

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Evaluation of whole cell and subcellular vaccines against Brucella ovis in rams

Cited by 46 publications

References 26 publications

Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines

Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines

The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

Radiomarcaje y estudios de biodistribución de nanopartículas poliméricas como adyuvantes para la vacunación oftálmica frente a la brucelosis

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