Evaluation of Wild Yam (Dioscorea villosa) Root Extract as a Potential Epigenetic Agent in Breast Cancer Cells

Aumsuwan, Pranapda; Khan, SI; Khan, IA; Walker, LA; Dasmahapatra, AK

doi:10.1055/s-0033-1336510

Cited by 2 publications

(8 citation statements)

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“…Previously, we have observed that wild yam root extract (WYRE) is capable of modulating DNA methylation in human breast cancer cell lines (Aumsuwan et al 2015). However, the exact mechanisms by which WYRE reduced DNA methylation are unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Cell culture conditions (under specific hormone-free experimental conditions) and the cellular isolation of RNA was described previously (Aumsuwan et al, 2015). In brief, human breast adenocarcinoma, MCF-7 (ER + ) and MDA-MB-231 (ER  ) cells were purchased from American type culture collection (ATCC).…”

Section: Cell Culture and Extraction Of Nucleic Acidmentioning

confidence: 99%

“…After complete removal of the media at 72h of treatment, the cells were trypsinized, resuspended in the medium, washed twice with PBS, and were used for biochemical analysis. RNA was extracted by Trizol reagent (Invitrogen, Carlsberg, CA) as described previously (Aumsuwan et al, 2015). The extracted RNA was dissolved in nuclease-free water.…”

Section: Cell Culture and Extraction Of Nucleic Acidmentioning

confidence: 99%

“…Gene-specific mRNA analysis was done by RT-qPCR methods based on PCR array (SA BioScience, Valencia, CA) (Aumsuwan et al, 2015). Briefly, the extracted RNA was reverse transcribed to cDNA by iScript Reverse transcriptase following the manufacturer's protocol (Bio-Rad, Hercules, CA).…”

Section: Quantitative Real-time Pcr (Rt-qpcr)mentioning

confidence: 99%

“…Previously, we have observed that wild yam (Dioscoria villosa) root extract (WYRE) has the potential to modulate epigenetic events in human breast cancer cell lines (Aumsuwan et al, 2015). Moreover, WYRE has the ability to activate GATA3 in both estrogen receptor positive (ER + ) MCF-7 cells and estrogen receptor negative (ER  ) MDA-MB-231 cells.…”

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confidence: 99%

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The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro

Aumsuwan

Khan

et al. 2016

Archives of Biochemistry and Biophysics

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Previously, we observed that wild yam (Dioscorea villosa) root extract (WYRE) was able to activate GATA3 in human breast cancer cells targeting epigenome. This study aimed to find out if dioscin (DS), a bioactive compound of WYRE, can modulate GATA3 functions and cellular invasion in human breast cancer cells. MCF-7 and MDA-MB-231 cells were treated in the absence/presence of various concentrations of DS and subjected to gene analysis by RT-qPCR, immunoblotting, and immunocytochemistry. We determined the ability of MDA-MB-231 cells to migrate into wound area and examined the effects of DS on cellular invasion using invasion assay. DS reduced cell viability of both cell lines in a concentration and time-dependent manner. GATA3 expression was enhanced by DS (5.76 μM) in MDA-MB-231 cells. DS (5.76 μM)-treated MDA-MB-231 cells exhibited the morphological characteristic of epithelial-like cells; mRNA expression of DNMT3A, TET2, TET3, ZFPM2 and E-cad were increased while TET1, VIM and MMP9 were decreased. Cellular invasion of MDA-MB-231 was reduced by 65 ± 5% in the presence of 5.76 μM DS. Our data suggested that DS-mediated pathway could promote GATA3 expression at transcription and translation levels. We propose that DS has potential to be used as an anti-invasive agent in breast cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Cell Culture and Extraction Of Nucleic Acidmentioning

confidence: 99%

Section: Cell Culture and Extraction Of Nucleic Acidmentioning

confidence: 99%

Section: Quantitative Real-time Pcr (Rt-qpcr)mentioning

confidence: 99%

mentioning

confidence: 99%

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The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro

Aumsuwan

Khan

et al. 2016

Archives of Biochemistry and Biophysics

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Plant natural modulators in breast cancer prevention: status quo and future perspectives reinforced by predictive, preventive, and personalized medical approach

et al. 2018

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In contrast to the genetic component in mammary carcinogenesis, epigenetic alterations are particularly important for the development of sporadic breast cancer (BC) comprising over 90% of all BC cases worldwide. Most of the DNA methylation processes are physiological and essential for human cellular and tissue homeostasis, playing an important role in a number of key mechanisms. However, if dysregulated, DNA methylation contributes to pathological processes such as cancer development and progression. A global hypomethylation of oncogenes and hypermethylation of tumor-suppressor genes are characteristic of most cancer types. Moreover, histone chemical modifications and non-coding RNA-associated multi-gene controls are considered as the key epigenetic mechanisms governing the cellular homeostasis and differentiation states. A number of studies demonstrate dietary plant products as actively affecting the development and progression of cancer. BNutri-epigenetics^focuses on the influence of dietary agents on epigenetic mechanisms. This approach has gained considerable attention; since in contrast to genetic alterations, epigenetic modifications are reversible affect early carcinogenesis. Currently, there is an evident lack of papers dedicated to the phytochemicals/plant extracts as complex epigenetic modulators, specifically in BC. Our paper highlights the role of plant natural compounds in targeting epigenetic alterations associated with BC development, progression, as well as its potential chemoprevention in the context of preventive medicine. Comprehensive measures are stated with a great potential to advance the overall BC management in favor of predictive, preventive, and personalized medical services and can be considered as Bproof-of principle^model, for their potential application to other multifactorial diseases.

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Evaluation of Wild Yam (Dioscorea villosa) Root Extract as a Potential Epigenetic Agent in Breast Cancer Cells

Cited by 2 publications

References 0 publications

The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro

The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro

Plant natural modulators in breast cancer prevention: status quo and future perspectives reinforced by predictive, preventive, and personalized medical approach

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