Evaluation of Wound Closure Rates Using a Human Fibroblast-derived Dermal Substitute Versus a Fetal Bovine Collagen Dressing: A Retrospective Study

Fitzgerald, Ryan H; Sabolinski, Michael L.; Skornicki, Michelle

doi:10.25270/wmp.2019.9.2634

Cited by 7 publications

(6 citation statements)

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“…Baseline characteristics of wounds treated included those that were of long duration (96 days), large size (12.9 cm 2 ), deep (5.2 mm) and high volume (11.2 cm 3 ). These wound measurement parameters at the first day of treatment application have previously been shown to be negative risk factors for healing (Table 1) [48][49][50]. It appears that PCMP successfully resulted in wound closure in a patient population who most often present with difficult-to-heal wounds refractory to previous treatments.…”

Section: Discussionmentioning

confidence: 84%

Type I collagen matrix plus polyhexamethylene biguanide antimicrobial for the treatment of cutaneous wounds

et al. 2020

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Aim: Determine the effectiveness of purified native type I collagen matrix plus polyhexamethylene biguanide antimicrobial (PCMP) on cutaneous wounds. Materials & methods: A prospective cohort study of 307 patients (67 venous leg ulcers, 62 diabetic foot ulcers, 45 pressure ulcers, 54 postsurgical wounds and 79 other wounds) was conducted. Results: Cox wound closure for PCMP was 73% at week 32. The median time to wound closure was 17 weeks (Kaplan–Meier). The incidence of PCMP-treated wounds showing >60% reductions in areas, depths and volumes were 81, 71 and 85%, respectively. Conclusion: PCMP demonstrated clinically meaningful benefits to patients with various types of cutaneous wounds. Clinical Trial registration number: NCT03286452.

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Section: Discussionmentioning

confidence: 84%

Type I collagen matrix plus polyhexamethylene biguanide antimicrobial for the treatment of cutaneous wounds

et al. 2020

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“…When comparing BSS varieties, there were a total of five articles, four of which compared cellular BSS to acellular BSS [ 41 , 51 , 63 , 64 ]. In those four, cellular BSSs demonstrated faster healing times and increased healing rates; however, they were vastly more expensive in two of those studies [ 51 , 64 ] [ Table 2 ].…”

Section: Resultsmentioning

confidence: 99%

Bioengineered Skin for Diabetic Foot Ulcers: A Scoping Review

Primous,

Elvin,

Carter

et al. 2024

JCM

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Diabetic foot ulcers (DFUs) pose a significant threat to individuals with diabetes mellitus (DM), such as lower limb amputation and severe morbidity. Bioengineered skin substitutes (BSS) are alternatives to traditional interventions for treating DFUs, but their efficacy compared to standard wound care (SWC) or other treatment types, such as allografts, remains unknown. A scoping review of human studies was conducted to identify current approaches in the treatment of DFUs using BSS as compared with other treatment options. Systematic searches in PubMed, Cochrane Library, and Web of Science were conducted to identify comparative studies that enrolled 10 or more patients and evaluated wound healing outcomes (closure, time-to-healing, and area reduction). Database searches isolated articles published from 1 December 2012 to 1 December 2022 and were conducted in accordance with PRISMA-ScR guidelines. The literature search yielded 1312 articles, 24 of which were included for the qualitative analysis. Findings in these studies demonstrated that BSS outperformed SWC in all measured outcomes, suggesting that BSS may be a superior treatment for DFUs. Of the 24 articles, 8 articles compared human amniotic membrane allografts (hAMA) to BSS. Conflicting evidence was observed when comparing BSS and hAMA treatments, highlighting the need for future research.

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“…The use of MSC, Fb, and Kc plays an important role in wound healing, and they were used as in vitro models to analyze the possible cytotoxic effect of AgNPs, AgNPs reduce cell viability but at 125 ppm most of the cells can adhere to the scaffold and present adequate viability; however, AgNPs should be handled with caution [ 28 , 29 ]. The use of materials of biological origin like RPS, promotes a better interaction without leaving adverse effects on the damaged tissue, in addition, this type of material is eventually detached, which allows the incorporation of the cells naturally in the affected area [ 30 ]. One important result is that the incorporation of AgNPs to RPS allowed the closure of the wound in the animal model, showing that the AgNPs’ concentration applied here did not interfere with the wound healing process and also enhanced ECM deposition ( Figure 5 , Figure 6 and Figure S3 and S4 ).…”

Section: Discussionmentioning

confidence: 99%

Radiosterilized Pig Skin, Silver Nanoparticles and Skin Cells as an Integral Dressing Treatment for Burns: Development, Pre-Clinical and Clinical Pilot Study

Ortega-Sánchez,

Pérez-Díaz,

Melgarejo-Ramírez

et al. 2023

Pharmaceutics

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Radiosterilized pig skin (RPS) has been used as a dressing for burns since the 1980s. Its similarity to human skin in terms of the extracellular matrix (ECM) allows the attachment of mesenchymal stem cells, making it ideal as a scaffold to create cellularized constructs. The use of silver nanoparticles (AgNPs) has been proven to be an appropriate alternative to the use of antibiotics and a potential solution against multidrug-resistant bacteria. RPS can be impregnated with AgNPs to develop nanomaterials capable of preventing wound infections. The main goal of this study was to assess the use of RPS as a scaffold for autologous fibroblasts (Fb), keratinocytes (Kc), and mesenchymal stem cells (MSC) in the treatment of second-degree burns (SDB). Additionally, independent RPS samples were impregnated with AgNPs to enhance their properties and further develop an antibacterial dressing that was initially tested using a burn mouse model. This protocol was approved by the Research and Ethics Committee of the INRLGII (INR 20/19 AC). Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis of the synthesized AgNPs showed an average size of 10 nm and rounded morphology. Minimum inhibitory concentrations (MIC) and Kirby–Bauer assays indicated that AgNPs (in solution at a concentration of 125 ppm) exhibit antimicrobial activity against the planktonic form of S. aureus isolated from burned patients; moreover, a log reduction of 1.74 ± 0.24 was achieved against biofilm formation. The nanomaterial developed with RPS impregnated with AgNPs solution at 125 ppm (RPS-AgNPs125) facilitated wound healing in a burn mouse model and enhanced extracellular matrix (ECM) deposition, as analyzed by Masson’s staining in histological samples. No silver was detected by energy-dispersive X-ray spectroscopy (EDS) in the skin, and neither by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in different organs of the mouse burn model. Calcein/ethidium homodimer (EthD-1), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and scanning electron microscopy (SEM) analysis demonstrated that Fb, Kc, and MSC could attach to RPS with over 95% cell viability. Kc were capable of releasing FGF at 0.5 pg above control levels, as analyzed by ELISA assays. An autologous RPS-Fb-Kc construct was implanted in a patient with SDB and compared to an autologous skin graft. The patient recovery was assessed seven days post-implantation, and the patient was followed up at one, two, and three months after the implantation, exhibiting favorable recovery compared to the gold standard, as measured by the cutometer. In conclusion, RPS effectively can be used as a scaffold for the culture of Fb, Kc, and MSC, facilitating the development of a cellularized construct that enhances wound healing in burn patients.

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Evaluation of Wound Closure Rates Using a Human Fibroblast-derived Dermal Substitute Versus a Fetal Bovine Collagen Dressing: A Retrospective Study

Cited by 7 publications

References 0 publications

Type I collagen matrix plus polyhexamethylene biguanide antimicrobial for the treatment of cutaneous wounds

Type I collagen matrix plus polyhexamethylene biguanide antimicrobial for the treatment of cutaneous wounds

Bioengineered Skin for Diabetic Foot Ulcers: A Scoping Review

Radiosterilized Pig Skin, Silver Nanoparticles and Skin Cells as an Integral Dressing Treatment for Burns: Development, Pre-Clinical and Clinical Pilot Study

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