Evaluation of wound healing activity of plumbagin in diabetic rats

Shao, Yi; Dang, Minyan; Lin, Yukiat; Xue, Feng

doi:10.1016/j.lfs.2019.04.048

Cited by 51 publications

(32 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is essential for wound healing to maintain the balance of ROS. Shao et al studied the lipid peroxidation rate of diabetic rats using Plumbagin, and the results indicated that Plumbagin significantly accelerated wound healing by increasing the levels of antioxidant enzymes such as SOD, CAT, GPx, GR, and GST in diabetic rats [10]. The study of Abood et al revealed that external use of P. macrocarpa fruit extract can accelerate wound healing and reduce tissue injury by increasing the activity of SOD, CAT, and MDA antioxidant enzymes [11].…”

Section: Introductionmentioning

confidence: 99%

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

et al. 2019

View full text Add to dashboard Cite

The structure of pepsin-solubilized collagen (PSC) obtained from the skin of Lophius litulon was analyzed using the sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). SDS-PAGE results showed that PSC from Lophius litulon skin was collagen type I and had collagen-specific α1, α2, β, and γ chains. FTIR results indicated that the infrared spectrum of PSC ranged from 400 to 4000 cm−1, with five main amide bands. SEM revealed the microstructure of PSC, which consisted of clear fibrous and porous structures. In vitro antioxidant studies demonstrated that PSC revealed the scavenging ability for 2,2-diphenyl-1-picrylhydrazyl (DPPH), HO·, O2−·, and ABTS·. Moreover, animal experiments were conducted to evaluate the biocompatibility of PSC. The collagen sponge group showed a good biocompatibility in the skin wound model and may play a positive role in the progression of the healing process. The cumulative results suggest that collagen from the skin of Lophius litulon has potential applications in wound healing due to its good biocompatibility.

show abstract

Section: Introductionmentioning

confidence: 99%

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

et al. 2019

View full text Add to dashboard Cite

show abstract

“…13,14 Plumbagin also exhibits potent cytotoxicity against various cancer cells, that is, cervical, lung, breast, pancreatic, oral, and prostate cancers. [15][16][17][18] Plumbagin possesses antiobesity activity, 19 wound healing activity in diabetic rats, 20 hepatoprotective activity, 21 and neuroprotective activity. 22 Besides these biological actions, the investigations regarding the therapeutic potential of plumbagin against the acute MI are not done yet.…”

mentioning

confidence: 99%

Plumbagin protects the myocardial damage by modulating the cardiac biomarkers, antioxidants, and apoptosis signaling in the doxorubicin‐induced cardiotoxicity in rats

Chinnathambi

Alharbi

et al. 2020

Environmental Toxicology

View full text Add to dashboard Cite

Cardiovascular disease created enormous health and economic burdens worldwide, which is responsible for the highest mobility and mortality that results in nearly 6.2% of in‐hospital deaths every year. Plumbagin is a major bioactive compound that occurs in the Plumbago indica and P. zeylanica with numerous therapeutic benefits. The current research exploration was planned to investigate the therapeutic role of plumbagin against doxorubicin stimulated cardiotoxicity in rats. The cardiotoxicity was stimulated to the rats by administering the 2.5 mg/kg of doxorubicin for 14 days with concurrent supplementation with plumbagin. The hemodynamic parameters were studied by using the tail‐cuff plethysmography. The lipid peroxidation and antioxidant status was examined by the standard procedures. The myocardial function and damage markers were assessed with the help of commercial kits. The expression status of inflammatory markers and PI3K/Akt signaling markers were investigated by reverse transcription polymerase chain reaction (RT‐PCR) and western blotting analysis, respectively. The plumbagin supplementation appreciably regained the body weight and heart weight of the investigational animals. Hemodynamic parameters and antioxidants statuses were escalated by the plumbagin treatment. The severe elevation in the cardiac damage markers and inflammatory markers were noticeably ameliorated by the plumbagin treatment. The plumbagin treatment also assuaged the overexpression of inflammatory and apoptotic proteins in the heart tissues of doxorubicin‐challenged rats. The histopathological analysis revealed that the plumbagin appreciably protected the heart tissues from the doxorubicin‐induced damages. The findings of this exploration evidenced that plumbagin treatment attenuated the doxorubicin‐stimulated cardiotoxicity in rats.

show abstract

“…Nrf2 (NF-E2-related factor) is an important transcription factor that maintains redox homeostasis in cells, participates in the development of various diseases, and plays an important role in the regulation of oxidative stress [16,42,45].At the same time, related reports indicate that Nrf2 is involved in the proliferation, differentiation, migration and apoptosis of various types of cells [5,46]. The exosomes of adipose-derived stem cells that highly express Nrf2 can promote the healing of diabetic foot ulcer skin wounds in rats [47,48].In vivo and in vitro experiments, we found that LPS-Exos can activate Nrf2 / HO-1 pathway-related proteins, while reducing in ammation-related proteins, promoting endothelial cell vascularization and collagen synthesis.Interestingly, after treatment with exosome-speci c inhibitors (GW4869), the symptoms improved by LPS-Exos were all reversed.…”

Section: Discussionmentioning

confidence: 99%

LPS-stimulated Macrophage Exosomes Inhibit Inflammation by Activating the Nrf2 / HO-1 Defense Pathway and Promote Wound Healing in Diabetic Rats

Tian

et al. 2020

Preprint

View full text Add to dashboard Cite

Background:Impaired wound healing is one of the important complications of diabetes. However, the specific pathogenesis is still unclear, and there is no effective treatment. Macrophages pretreated with chemical or biological factors may increase the biological activity of macrophage-derived exosomes, which is expected to become a new effective treatment method. The purpose of this study was to investigate whether the exosomes secreted by macrophages pretreated with lipopolysaccharide (LPS) have better anti-inflammatory and angiogenic abilities in the treatment of diabetic wound healing and their underlying molecular mechanisms.Methods:In this study, macroscopical, biochemical, histological, immunofluorescence and molecular biology methods were used to evaluate the potential protective mechanism and effect of lipopolysaccharide stimulated macrophage exosomes (LPS-Exos) on wound healing in streptozotocin induced hyperglycemia rats.In the in vivo experiment, the percentages of wound closure and contraction were compared and analyzed on the 7th, 14th, and 21st day after treatment by grouping treatments with different concentrations of LPS-Exos.At the same time, hematoxylin and eosin staining (HE), Masson staining, immunofluorescence staining and Western blotting (WB) were used for histological analysis of the wound tissue on the 14th day after injury to evaluate the impact of different treatment methods on wound healing.In in vitro experiments, the ability of endothelial cells related to proliferation, migration, tube formation and the expression of vascular endothelial growth factor (VEGF) were tested.At the same time, in vivo and in vitro experiments, the effect of Nrf2/HO-1 signaling pathway on LPS-Exos in inhibiting inflammation and promoting angiogenesis was evaluated by using exosome-specific inhibitors.Results:LPS-Exos reduced the content of ROS and MDA in the wound tissue of hyperglycemic rats, increased the activity of SOD and the production of GSH-Px, activated the Nrf2/HO-1 pathway, inhibited the expression of inflammation-related proteins, and promoted blood vessels generate. The group given exosome inhibitors reversed this phenomenon.Conclusions: LPS-Exos may activate the Nrf2/HO-1 defense pathway, improve endothelial cell function, inhibit oxidative damage and inflammation, and promote wound healing in diabetic rats, thereby having the potential to treat diabetic skin defects.

show abstract

Evaluation of wound healing activity of plumbagin in diabetic rats

Cited by 51 publications

References 86 publications

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Plumbagin protects the myocardial damage by modulating the cardiac biomarkers, antioxidants, and apoptosis signaling in the doxorubicin‐induced cardiotoxicity in rats

LPS-stimulated Macrophage Exosomes Inhibit Inflammation by Activating the Nrf2 / HO-1 Defense Pathway and Promote Wound Healing in Diabetic Rats

Contact Info

Product

Resources

About

Evaluation of wound healing activity of plumbagin in diabetic rats

Cited by 51 publications

References 86 publications

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Plumbagin protects the myocardial damage by modulating the cardiac biomarkers, antioxidants, and apoptosis signaling in the doxorubicin‐induced cardiotoxicity in rats

LPS-stimulated Macrophage Exosomes Inhibit Inflammation by Activating the Nrf2&nbsp;/ HO-1 Defense Pathway and Promote Wound Healing in Diabetic Rats

Contact Info

Product

Resources

About

LPS-stimulated Macrophage Exosomes Inhibit Inflammation by Activating the Nrf2 / HO-1 Defense Pathway and Promote Wound Healing in Diabetic Rats