Evaluation of wound healing effect of colostrum and curcumin cream in rat excision wound model

Sakthibalan, M; Aakash, R; Adhimoolam, Mangaiarkkarasi; Prasad, Charushree

doi:10.18231/j.joapr.2022.15.20

Cited by 1 publication

(1 citation statement)

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“…Type I collagen deposition and remodeling was recognized as a contributor in increasing tensile strength of the wound. 47 Many natural extracts explored towards wound healing also induced the expression and improved morphology of collagen type I in the healing wound tissue. 48 Inadequate ECM production leads to pathological condition that results in premature aging.…”

Section: Discussionmentioning

confidence: 99%

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Tanimu,

Zirmire K,

Jamora

et al. 2024

Preprint

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Wound healing is a complex process that requires a well-orchestrated integration of an array of molecular events such as cell migration and proliferation, deposition and remodeling of extracellular matrix components for restoring the structural and functional integrity of the tissue injured. Ayurveda suggests wound healing herbs can achieve enhanced therapeutic effect with reduced toxicity when they are optimally combined in a specific ratio as polyherbal formulation (PHF). The present study was aimed to evaluate the combinatorial wound healing efficacy (invivowound closure and histological changes) of aqueous extracts of three medicinal plants (Bacopa monnieri,Acalypha indicaandCalotropis gigantea). This study also explored how the combination influenced the overall quality of healed wound. Individual wound closure kinetic performance of aqueous plant extracts in C57B/6J mice was assessed using safe concentrations obtained from human adult dermal fibroblast viability assay. The aqueous plant extract combination optimized using response surface methodology was tested forinvivowound closure effectiveness. Quality of healed wound was assessed via Hematoxylin & Eosin and immunohistochemical staining of markers (K1, K5, Loricrin, Ki67, CD31 and collagen1). The combination treatment (B.monnieri-15μg/ml,A.indica-11.59μg/ml,C.gigantea-1μg/ml) contributed to faster wound closure (11 days), improved collagen type I remodeling and angiogenesis, complete re-epithelialization, similar epidermal differentiation pattern as that of individual and control treatments. Ki67 staining revealed no significant increase in cell proliferation in combination compared to individual and control. Findings from the study validates the polyherbal combination′s impressive capability to promote wound healing.

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Section: Discussionmentioning

confidence: 99%