Evaluation of Xanthine Oxidase Inhibitory Activity by Flavonoids From Pongamia Pinnata Linn

Abstract

Objective: Flavonoids from the crude seeds extract of Pongamia pinnata L., dried fruit powder of Morinda citrifolia L., bark of Mangifera indica L., and rhizome of Zingiber officinale Rosc. were screened for xanthine oxidase (XO) inhibition at different concentration. The inhibitory potential of quercetin and allopurinol were used for the determination of 50% inhibitory concentration (IC 50 ) and K i values.Methods: Isolation of flavonoids from the plant extracts was processed by column chromatography and tested for XO inhibitory activity in the range of 6-800 µg/ml. Results:The results demonstrated that optimized flavonoids extract of P. pinnata L. exhibited promising XO inhibition. P. pinnata L., M. indica L., and Z. officinale Rosc. had IC 50 in the concentration of 8.74 mM, 1.09 mM, 5.4 mM and Ki 0.35 mM, 1.73 mM, 2.7 mM, respectively. Conclusion:The study showed that plant species under investigation exhibited XO inhibition by optimized flavonoid extract. P. pinnata L. indicated promising XO inhibition compared to other plant extracts. Flavonoids can be used as a potent inhibitor of XO an alternative to allopurinol.

Spectroscopic Analysis of Flavonoids Isolated from Pongamia pinnata L. Seed Oil

“…Their use as diet, their antioxidant properties [16,17] or their estrogenic action, and to a wide range of antimicrobial and pharmacological activities and functionalization of enzymes [18,19]. Currently we have isolated flavonoids from seed oil of Pongamia pinnata (L.) which is a rich source of flavonoids [20][21][22][23][24][25]. Pongamia pinnata (L.) is widely distributed in the coastal region and plains of South Asia, South East Asia, Australia and in all the four provinces of Pakistan, especially in north Punjab [26,27].…”