Evaluation of Xpert MTB/ RIF Assay for the Detection of Female Genital Tuberculosis in a Tertiary Care Center- A Descriptive Cross-sectional Study

Farhana, Anjum; Zahoor, Danish; Manzoor, Munazah; Kanth, Farhath

doi:10.9734/mrji/2018/39636

Cited by 8 publications

(9 citation statements)

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“…The mean age of participants was 26.7 years in infertility and 35.7 years in the group with menstrual irregularity. The age and clinical presentations were consistent with those of other similar studies [3,7]. Specimens were collected from all patients suspected of FGTB based on their symptomatology and preoperative findings.…”

Section: Discussionsupporting

confidence: 66%

“…The incidence of FGTB ranges from 1%-19% in different parts of India [2]. Infertility is the most common symptom of FGTB, and the incidence of FGTB in infertility varies from 3%-16% in India [3]. Given the high prevalence of diseases in patients with infertility and menstrual disorders, a high degree of clinical suspicion for FGTB should be considered.…”

Section: Introductionmentioning

confidence: 99%

“…Given the high prevalence of diseases in patients with infertility and menstrual disorders, a high degree of clinical suspicion for FGTB should be considered. Therefore, detailed history, systemic examination, and series of investigations are needed [3]. Many diagnostic modalities have been developed although diagnosing FGTB remains challenging due to its paucibacillary nature.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Efficacy of the Cartridge-Based Nucleic Amplification (CBNAAT)/Xpert Test and Histology of Genital Tissues in Diagnosing Female Genital Tuberculosis

Kanti¹,

Seth²,

Gupta

et al. 2021

Cureus

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BackgroundDiagnosing female genital tuberculosis (FGTB) is very difficult by routine laboratory investigations. Collecting tissues from genital structures, especially from tubes for histology, is impossible. The cartridgebased nucleic amplification (CBNAAT)/Xpert RIF test is a new polymerase chain reaction (PCR)-based method that is quick and may diagnose FGTB from any tissue type; however, it should not be contaminated with blood. This study was conducted to compare the efficacy of CBNAAT and the histology of genital tissue in suspected cases. Materials and methodsThis was a prospective study of the diagnostic efficacy of 91 cases of suspected FGTB randomly selected from March 2018 to September 2019 at a rural tertiary care center. Endometrial tissue collected in 86 patients (59 infertility, 27 menstrual irregularities) and tubal/peritoneal tissue from hysterectomy or laparotomy specimens of five participants who underwent surgery were sent for histopathological analysis and CBNAAT and the results were evaluated and compared. ResultsThere were 59 (64.83%) and 32 (35.2%) cases of infertility and menstrual irregularities, respectively. Primary infertility (38; 41.75%) was the most common complaint. Endometrial biopsies (EB) of two (2.23%) cases were found positive for tuberculosis (TB) both on histopathological examination (HPE) and CBNAAT. In addition, both patients had primary infertility. Of the 32 cases with menstrual abnormalities (27 EB and three tubal tissue, two peritoneal and nodular tissue), none were found to be positive for TB on HPE or CBNAAT. A highly significant association was found between histopathology and CBNAAT (p<0.0001) in the endometrial tissue of infertile patients. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 100% for CBNAAT, with reference to histopathology. ConclusionWe recommend CBNAAT for the early detection of FGTB, with the added advantage of early results, minimal technical expertise, and detection of drug-resistant tuberculosis (TB).

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of the Efficacy of the Cartridge-Based Nucleic Amplification (CBNAAT)/Xpert Test and Histology of Genital Tissues in Diagnosing Female Genital Tuberculosis

Kanti¹,

Seth²,

Gupta

et al. 2021

Cureus

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“…Two samples that were positive on culture were found negative on GeneXpert which could be because of blood contamination of endometrial biopsy sample as quoted by the authors. However, due to higher rate of detection and minimal technical expertise GeneXpert was recommended by the authors for rapid diagnosis and detection of drug resistant tuberculosis in genital tuberculosis 4 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, country like India having the highest incidence of tuberculosis in the world needs early and aggressive strategies to diagnose and treat tuberculosis. Diagnosis of female genital tuberculosis by microscopy and culture is difficult due to its paucibacillary nature 4 . Histopathological diagnosis demonstrates typical caseous granulomatous lesions with giant epitheloid cells suggestive of tuberculosis but not diagnostic as these also appear in fungal infections and sarcoidosis 5 .…”

mentioning

confidence: 99%

GeneXpert positivity and evaluation of rifampicin resistance among endometrial biopsy specimens for the diagnosis of Genital Tuberculosis

Kashyap¹,

Dibyashree²,

Saha³

et al. 2020

OBGYN

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Objective: The aim of this study was to find out the GeneXpert positivity among endometrial biopsy specimens for diagnosis of genital tuberculosis. Materials and Methods: A retrospective study was conducted from January 2019 to June 2019 on all women clinically and radiologically diagnosed with infertility, dyspareunia, menstrual irregularities and pelvic inflammatory disease. Endometrial biopsies were taken from all cases and subjected to GeneXpert test for detection of Mycobacterium tuberculosis. Result: Total number of suspected cases was 62 with age ranging from 19 years to 65 years. Majority of suspected cases were from the age group 21-30 years (54.8%). Five out of 62 (8%) cases tested positive for Mycobacterium tuberculosis by GeneXpert which were all sensitive to Rifampicin. Among these 5 cases, 2 were reported as medium positive and 3 as very low positive based on cycle threshold value. Conclusion: GeneXpert on endometrial biopsy may prove to be a reliable diagnostic modality for genital tuberculosis as well as multidrug resistance tuberculosis in females leading to timely management of the disease.

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Female genital tuberculosis in light of newer laboratory tests: A narrative review

Munne

Tandon

Chauhan

et al. 2020

Indian Journal of Tuberculosis

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Evaluation of Xpert MTB/ RIF Assay for the Detection of Female Genital Tuberculosis in a Tertiary Care Center- A Descriptive Cross-sectional Study

Cited by 8 publications

References 0 publications

Comparison of the Efficacy of the Cartridge-Based Nucleic Amplification (CBNAAT)/Xpert Test and Histology of Genital Tissues in Diagnosing Female Genital Tuberculosis

Comparison of the Efficacy of the Cartridge-Based Nucleic Amplification (CBNAAT)/Xpert Test and Histology of Genital Tissues in Diagnosing Female Genital Tuberculosis

GeneXpert positivity and evaluation of rifampicin resistance among endometrial biopsy specimens for the diagnosis of Genital Tuberculosis

Female genital tuberculosis in light of newer laboratory tests: A narrative review

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