Evaluation ofthe additive effect ofthe interaction of phytobiotics with zinc on <i>Quorum Sensing P. aeruginosa</i> in an <i>in vitro</i> mode

Abstract

Relevance. The development of the aquaculture sector is one of the promising developing areas that contribute to ensuring the food security of mankind in the world. However, all aquatic animals are susceptible to the impact of negative factors leading to a decrease in growth rates, a decrease in the quality of finished products, etc. Methods. The paper presents experimental data on the study of the additive effect of a combination of various commercial phytobiotic drugs with zinc on the sense of quorum and inhibitory characteristics on a model of a polyresistant strain of P. aeruginosa. The choice of the strain is due to its high resistance characteristics, ability to biofilm formation, as well as the ability to visually assess the impact of the tested compounds on the Quorum Sensing (QS) system by suppressing the formation of the pyocyanin pigment, which provides virulence factors and biofilm growth. Butitan, Probiocid®-Phyto, and Intebio preparations were used as factors regulating the growth of the tested strain; ZnSO4 was used as a source of zinc cations. The use of the diffusion method of agar wells allowed us to evaluate not only the level of the inhibitory effect of the studied compounds, but also the presence of their influence (QS) of the system. Results. The experimental data obtained indicate a pronounced effect of zinc cations on the production of the pyocyanin pigment (0.25 mM/ml), as well as the tested preparations from the group of fodder phytobiotics at concentrations of 100 mg/ml. The presence of significantly significant differences (р ≤ 0.001) in the impact on (QS) in combinations of zinc with phytobiotics at concentrations of 0.13 mM/ml ZnSO4 and extracts of drugs 50 mg/ml was established, with the highest rates in “Probiocid®-Phyto”. Thus, the data obtained allow us to conclude that the use of the studied phytobiotics in combination with essential elements is promising as an alternative to feed antibiotics in fish feeding, for the prevention of infectious diseases.