The integration of industrial chain and innovation chain is an important way to improve the stability and creative development of industrial chain. The integration of industrial chain and innovation chain is an important strategic direction of China's future economic development, and it is of far-reaching significance to explore the impact of "two-chain integration" on the innovation ability of electronic information enterprises. This paper explores the influence of two-chain fusion on the innovation ability of electronic information enterprises from three dimensions: fusion path, fusion effect and fusion mechanism. Based on the structural equation model analysis of 252 questionnaire data, the results show that the integration of two chains and knowledge management have positive effects on the innovation ability of electronic information enterprises. The integration of the two chains also has a positive impact on knowledge management. The research conclusion has important practical guiding significance for improving the innovation ability of electronic information enterprises in the Beijing-Tianjin-Hebei region and promoting the transformation and upgrading of electronic information industry in the Beijing-Tianjin-Hebei region.