Evaluation on Sperm Acrosome Integrity of Infertile Men with Varicocele

Tzvetkova, Pavleta; Zhu, Weijie; Li, Jing; Tzvetkov, D

doi:10.1016/s1001-7844(07)60003-0

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…Spermatozoa that have lost acrosome long before encountering zona pellucida cannot perform these two functions hence fail to fertilize. Moreover spermatozoa die shortly after acrosomal exocytosis [22]. The present work has shown that Artemisia leaf extract causes precocious acrosome exocytosis in mouse spermatozoa.…”

Section: Discussion (Optional)supporting

confidence: 54%

Artemisia vulgaris extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa

Bhandari¹,

Sharma²,

Rizal³

et al. 2021

J Anim Sci Technol

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Several herbs including Artemisia are known to possess conceptive property. In the present study, mouse spermatozoa were incubated with ethanol extract of Artemisia vulgaris leaves. The effect of extract on acrosome exocytosis was studied by labeling spermatozoa with fluorescein isothiocyanate (FITC) peanut agglutinin and by staining with Coomassie blue. Viability and membrane integrity were studied by Trypan-blue staining and hypo-osmotic swelling test. Artemisia extract at very low concentration caused precocious acrosome reaction and loss of sperm viability. Acrosome reaction increased remarkably from 22.63% to 88.42% with increasing extract concentration from 0 to 2,000 µg/mL. However, the viability loss of spermatozoa was increased from 11.71% in control to 63.73% in samples treated, evaluated by Trypan-blue staining method. Membrane damage caused by the extract, evaluated by hypo-osmotic swelling test was even low, ranging from 2.27% to only 24.23%. These results indicate that Artemisia extract might block fertilization by causing precocious acrosome exocytosis in spermatozoa. A direct contraceptive effect was tested by injecting the plant extract into the vagina of female mice and then allowing them to mate with normal males. The treated female mice delivered significantly fewer litters in comparison to the control.

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Section: Discussion (Optional)supporting

confidence: 54%

Artemisia vulgaris extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa

Bhandari¹,

Sharma²,

Rizal³

et al. 2021

J Anim Sci Technol

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“…The acrosome area of spermatozoa contains various proteins that play an essential role in the acrosome reaction and penetration with the zona pellucida [ 50 ]. Acrosomal integrity is important to prevent premature acrosomal reactions and low acrosomal integrity has been associated with infertility [ 51 ]. According to Bailey et al [ 52 ], freeze-thawing causes capacitation-like reactions in mammalian spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Correlation between Post‐Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression

Yoelinda

Arifiantini

Solihin

et al. 2023

Veterinary Medicine International

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The role of ex situ conservation facilities or captivity through captive breeding programs is essential in the conservation of the endangered Javan banteng. The development of semen cryopreservation may assist on one side of the conservation plan. However, the male Javan banteng reproductive capability must be considered as it influences the targeted outputs. Studying the potential biomarker for fertility such as osteopontin gene expression is also expected to help predict male fertility. Therefore, this study aimed to analyze the quality of spermatozoa after thawing to help predict the male reproductive capability of Javan banteng. Furthermore, this study investigated the potential role of osteopontin gene expression in male Javan banteng fertility. A positive reinforcement approach was used to accustom the male and female animals as we focused on establishing a collection procedure using neither sedation nor anaesthesia. Semen samples were collected at Taman Safari Indonesia, Bogor, in accordance with the female banteng receptivity. Semen samples were then evaluated and then cryopreserved under field conditions. Our study showed the different predicted reproductive capability of the Javan banteng based on the post-thaw spermatozoa quality, which showed significant differences. The OPN gene showed positive correlations with the progressive motility (r = 0.711, p = 0.048), viability (r = 0.822, p = 0.012), and acrosomal integrity (r = 0.665, p = 0.072) of Javan banteng spermatozoa after thawing. Our study demonstrated the predicted Javan banteng reproductive capability based on various post-thaw spermatozoa variables. This finding is also the first report on the OPN gene potential to be developed as the assessment tool of post-thaw spermatozoa quality of the male Javan banteng. The findings in our study may help give recommendations for future breeding programs, especially in the ex situ conservation sites.

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“…Low acrosomal integrity of sperm has been seen in many infertile cases and may be attributed due to low level of acrosin activty. 39 An intact acrosome is necessary for the sperm to fertilize an egg. Before fertilization, acrosome reaction takes place in sperm cell and enzymes present in acrosome becomes activated making a hole in egg zona pellucida allowing sperm to penetrate and to bind with plasma membrane of oocyte.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Extenders for the Liquid Storage of Ring-Necked Pheasant (Phasianuscolchicus) Semen

Mumtaz¹,

Razzaq²,

Ahmad³

et al. 2022

IJCRR

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Introduction: Ring-necked pheasant (Phasianuscolchicus) is a famous game bird and has ecological, marketable, aesthetic, sport and nutritive values. It is native to Eastern central Asia, China, Japan and is introduced as game bird to other regions of the world. Population of this species is continuously declining due to hunting pressure, habitat fragmentation, meat purpose and increased urbanization. There is need to conserve the species through techniques of semen banking as may provide a protected net against extinction. Objective/Aim: The current study was designed to asses range of extenders (Red fowl, Tselutin Poultry, Beltsville Poultry, Chicken semen, Lake and EK extenders) for storage of ring-necked pheasant semen at 5 °C in refrigerator for 48 hours. Methodology: Short-term semen storage method used for avian semen, as avian sperms are sensitive to higher 11temperature fluctuations during the cryopreservation process. Semen collection was made from trained and mature cocks and diluted in the Beltsville Poultry, Red fowl, Lake, EK, Tselutin Poultry and Chicken semen extenders, stored at 5°C and evaluated for sperm motility (%), plasma membrane integrity (%), viability (%), acrosome integrity (%) and DNA fragmentation at 0h, 3h, 6h, 24h and 48 h of storage. Results: The spermatozoa motility was higher (p<0.05) in RFE (72.50±3.23) and Tselutin poultry extender (53.75±2.39) as compared to other diluents; Beltsville poultry (41.25±1.25) and Lake (30.00 ± 2.04) whereas Membrane integrity was also higher (P<0.05) in RFE (80.50±2.1) and Tselutin poultry extender (63.75±2.39). However, RFE (67.00±1.22) maintained sperm viability (P<0.05) higher at all storage hours (0, 3, 6, 24 and 48) as compared to TPE (63.75±1.49). Data obtained from the current study were analyzed by using ANOVA and LSD. Conclusion: Hence, it is concluded that ring-necked pheasant semen can be stored at 5ºC for 48 hours in Red fowl extender for captive propagation of the species.

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Evaluation on Sperm Acrosome Integrity of Infertile Men with Varicocele

Cited by 4 publications

References 18 publications

Artemisia vulgaris extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa

Artemisia vulgaris extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa

Correlation between Post‐Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression

Evaluation of Extenders for the Liquid Storage of Ring-Necked Pheasant (Phasianuscolchicus) Semen

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