The current study aims to clarify how much the measurement results using the image-based method are affected by two types of total overlaps in the images. By ensuring the homogeneous suspension of particles and simultaneous optimization of the probe location, it has been proven that using the overall holdup of particles as the benchmark data is reasonable. In a bidisperse particle system, the larger the particles, the more significant the effect of large particles veiling small particles. The maximal relative error nears 50%. In a poly-disperse system, the measurement error heavily depends on the veiled particle size. The larger the particles, the smaller the deviation. For the components of 1 and 3 mm, the relative errors under multiple conditions exceed 50%. The veiling effect of small particles on large particles is qualitatively evaluated. The results of the current study are helpful in promoting the inline image-based method for the actual multiphase reactor method.