Evaluation the effectiveness of Andrographis paniculata aqueous extract on hepatotoxicity and CD44 gene expression in male rabbits treated with butylated hydroxytoluene (BHT)

Abstract

This study is aimed at knowing the protective role of the aqueous extract of Andrographis paniculata on toxic effects that are induced by butylated hydroxytoluene (BHT) in domestic male rabbits. The study was conducted in College of Education for Pure Sciences / Univ. of Karbala from the beginning of November 2021 until February 2022. Forty adult male rabbits have been used, which were divided randomly into four equal groups (ten animals per group), the first group(G1) was administered 1ml/kg of corn oil and considered as the control group, the 2nd group (G2) administrated 1mg/kg of BHT, the third group (G3) administrated 100 mg/kg of A. paniculata, while the animals of the fourth group (G4) administrated 1mg/kg of BHT and 100mg/kg of A. paniculata extract orally for a month daily. Fasting Blood samples were collected after the end of experimentation in order to study the concentration of the following parameters: alanine transaminase (ALT), aspartate transaminase (AST), Tumor necrosis factor alpha (TNF-α), alkaline phosphatase (ALP), Cytochrome P450, and Interleukin 6 (IL6) in addition to measurement of the gene expression of CD44